重度冷应激对三河牛血液生化指标及相关基因表达的影响

doi:10.11843/j.issn.0366-6964.2015.08.024

Abstract

Abstract:

The aim of this study was to detect the biochemical indexes and relevant genes related to cold stress in Sanhe cattle.Thirty heifer Sanhe cattle in Inner Mongolia were kept at -32 ℃ for 3 h，and then blood samples were collected before and after cold stress separately to detect the biochemical indexes and gene expression changes.After 3 h cold stress，T3，T4 and ACTH in blood significantly increased (P<0.01).The expressions of LIN54 and KLF12 decreased in silver staining map produced by mRNA differential display method (DDRT)，decrease of the 2 genes expression were further verified at the level of P<0.05 for LIN54 and P<0.01 for KLF12 by fluorescence quantitative RT-PCR.The 2 genes belonged to LINC and KLF families，respectively,LINC involved in mitotic gene activation,KLF involved in cell proliferation and apoptosis.T3，T4 and ACTH may be used as the candidate indexes to measure the strength of cold stress in large population，LIN54 and KLF12 may be the important candidate genes for further study on genetic mechanism of cold stress in cattle.The combination of these biochemical indexes and expression change of candidate genes would be used to assess the change of individual under cold stress，which will be more valuable in breeding for cold resistance ability in Sanhe cattle.

CLC Number:

S823.2

LI Wei，LIU Rui，MA Yao，LI Jin-long，WU Hong-jun，LIU Ai-rong，YU Ying，XU Qing，WANG Ya-chun. Effects of Severe Cold Stress on Blood Biochemical Parameters and Related Gene Expression in Sanhe Cattle[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(8): 1463-1470.

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Effects of Severe Cold Stress on Blood Biochemical Parameters and Related Gene Expression in Sanhe Cattle

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