ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2013, Vol. 44 ›› Issue (12): 2029-2035.doi: 10.11843/j.issn.0366-6964.2013.12.025

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Influence of Sijunzi Decoction on Intestinal Flora Diversity in Spleen-deficient Rats

MENG Liang-yan1, CHEN Xiu-qin1, SHI Da-you1, HUANG Hai-ding1,2, GUO Shi-ning1*   

  1. (1.College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China;2.Laboratory of Animal Center, Guangzhou University of Traditional Medicine, Guangzhou 510405, China)
  • Received:2013-07-10 Online:2013-12-23 Published:2013-12-23

Abstract:

The aim of this study was to investigate and analyze the recuperative function of Chinese Medicine, Sijunzi decoction, on intestinal flora in spleen-deficient animals. Forty-eight rats were randomly divided into the Control group (n=12), the Spleen-deficient group (n=12), the Sijunzi decoction group (n=12) and the Sulfasalazine group (n=12). Spleen-deficiency rat model was caused by reserpine in all groups other than the Control group. A diarrhea model of rats was conducted followed by treatment of sijunzi decoction or SASP or non-treatment. Intestinal flora was determined at pre-modeling, pre- and post- treatment by a 16S rDNA gene sequencing. The results showed that the Sijunzi decoction improved the diversity of intestinal flora in diarrhea rats though the diversity of intestinal flora differs from that treated with SASP. The Sijunzi decoction is a potential medicine for treatment of intestinal flora disorder induced diarrhea.

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