ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2013, Vol. 44 ›› Issue (12): 1919-1925.doi: 10.11843/j.issn.0366-6964.2013.12.009

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Protective Effect of Vitamin E against Cell Apoptosis Induced by Serum-free Culturing in Granulosa Cells of Breeding Hen

ZHANG Hong-xin1, DI Ke-qian1,CHEN Hui2, HUANG Ren-lu2*   

  1. (1.Medical Experiment Center of Hebei University, Baoding 071000, China;2.College of Animal Science and Technology, Agricultural University of Hebei, Baoding 071000, China)
  • Received:2013-07-12 Online:2013-12-23 Published:2013-12-23

Abstract:

This study was conducted to investigate the effect of vitamin E (VE) against the cell apoptosis caused by serum-free culturing in granulosa cells from hen follicle. The granulosa cells were collected from the small yellow follicles of health breeding hens, which were at the egg producing peak within the regular cycle. After pre-incubation in 0.5% FCS mediumthe cells were cultured in serum-free medium containing 10 mg·L-1 vitamin E. The cell apoptosis and the cell cycle distribution of granulosa cells were detected by Flow Cytometry. The results showed that the concentration 10 mg·L-1 of vitamin E reduced the apoptosis of granulosa cells (P0.01), and increased the percentage of S phase (P0.05) compared with the control group. The results suggest that vitamin E can promote granulosa cells from G0/G1 phase to S phase and promote granular cells passing through G1/S period limit. Thereby the granulosa cell apoptosis of breeding hens induced by serum-free culture was inhibited.

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