亚硒酸钠对延边奶山羊卵母细胞体外成熟及体细胞克隆重构胚发育的影响

doi:10.11843/j.issn.0366-6964.2013.11.009

Abstract

Abstract:

The aim of the study was to investigate the effects of sodium selenite (SS) on in vitro maturation of Yanbian dairy goat oocytes and development of somatic cell nuclear transfer (SCNT). The oocytes were cultured in vitro medium supplemented with different concentrations (0.0,2.5,5.0，10.0 ng·mL^-1) of SS , then the oocytes maturation rate, intracellular ROS and GSH level were examined. The Yanbian dairy goat mammary gland epithelial cells were as the donor cells for SCNT. The quality and development capacity of SCNT embryos were evaluated with staining. The result showed that 5.0 ng·mL^-1 SS significantly improved the oocytes maturation rate and the SCNT embryos development compentence. The treatment with 5.0 ng·mL^-1 treatment was not only significantly reduced the ROS level but improved the GSH level and the blastocyst cell number. In conclusion, 5.0 ng·mL^-1 SS supplementation in vitro medium can significantly improved the Yanbian dairy goat oocytes maturation rate and SCNT embryos development competence.

CLC Number:

S827
S814.8

SUN Jing-tao, YIN Duo, LIU Hai-xing, LI Zhong-shu, FANG Nan-zhu. Effects of Sodium Selenite on the Yanbian Dairy Goat Oocytes in vitro Maturation and the Effects on the Development of Somatic Cell Nuclear Transfer Embryos[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(11): 1758-1765.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.xmsyxb.com/EN/10.11843/j.issn.0366-6964.2013.11.009

https://www.xmsyxb.com/EN/Y2013/V44/I11/1758

References

［1］BAVISTER B D. Culture of preimplantaion embryos: facts and artifacts ［J］. Hum Reprod, 1995, 1(2): 91-148.

［2］LUVONI G C, KESKINTEPE L, BRACKETT B G. Improvement in bovine embryo production in vitro by glutathione-containing culture media ［J］. Mol Reprod Dev, 1996, 43(4): 437-443.

［3］KRUIP T A, BEVERS M M, KEMP B. Environment of oocyte and embryo determines health of IVP offspring ［J］. Theriogenology, 2000, 53(2): 611-618.

［4］YOSHIDA M, ISHIGAKI K, PURSEL V. Effect of maturation media on male pronucleus formation in pig oocytes matured in vitro ［J］. Mol Reprod Dev, 1992, 31(1): 68-71.

［5］DE MATOS D G, FURNUS C C, MOSES D F, et al. Stimulation of glutathione synthesis of in vitro matured bovine oocytes and its effect on embryo development and freezability ［J］. Mol Reprod Dev, 1996, 45(4): 451-457.

［6］TATEMOTO H, SAKURAI N, MUTO N. Protection of porcine oocytes against apoptotic cell death caused by oxidative stress during in vitro maturation: role of cumulus cells ［J］. Biol Reprod, 2000, 63(3):805-810.

［7］TATEMOTO H, OOTAKI K, SHIGETA K, et al. Enhancement of developmental competence after in vitro fertilization of porcine oocytes by treatment with ascorbic acid 2-O-α-glucoside during in vitro maturation ［J］. Biol Reprod, 2001, 65(6):1800-1806.

［8］JAN?AR N, KOPITAR A N, IHAN A, et al. Effect of apoptosis and reactive oxygen species production in human granulosa cells on oocyte fertilization and blastocyst development ［J］. Assist Reprod Genet, 2007, 24(2-3):91-97.

［9］CASSANO E, TOSTO L, BALESTRIERIA M, et al. Antioxidant defense in the follicular fluid of water buffalo ［J］. Cell Physiol Biochem, 1999, 9(2):106-116.

［10］TATEMOTO H, OKUDA T, SOGO N, et al. Male pronuclear formation and blastocyst formation are improved by supplementation of ascorbic acid 2-O-α-glucoside during in vitro maturation cultured of denuded porcine oocytes ［J］. Reprod Dev, 2001, 47(6):329 -339.

［11］EBERT R, ULMER M, ZECK S, et al. Selenium supplementation restores the antioxidative capacity and prevents cell damage in bone marrow stromal cells in vitro ［J］. Stem Cells, 2006, 24(5):1226-1235.

［12］ZHANG J, ROBINSON D, SALMON P. A novel function for selenium in biological system: selenite as a highly effective iron carrier for Chinese hamster ovary cell growth and monoclonal antibody production ［J］. Biotechnol Bioeng, 2006, 95(6):1188-1197.

［13］TAKAHASHI K, COHEN H J. Selenium-dependent glutathione peroxidase protein and activity: immunological investigations on cellular and plasma enzymes ［J］. Blood, 1986, 68(3):640-645.

［14］UHM S J, GUPTA M K, YANG J H, et al. Selenium improves the developmental ability and reduces the apoptosis in porcine parthenotes ［J］. Mol Reprod Dev, 2007,74(11):1386-1394.

［15］GOPALAKRISHNA R, CHEN Z H, GUNDIMEDA U. Selenocompounds induce a redox modulation of protein kinase C in the cell, compartmentally independent from cytosolic glutathione: its role in inhibition of tumor promotion ［J］. Arch Biochem Biophys, 1997, 348(1):37-48.

［16］ABEDELAHI A, SALEHNIA M, ALLAMEH A A. The effects of different concentrations of sodium selenite on the in vitro maturation of preantral follicles in serum-free and serum supplemented media ［J］. Assist Reprod Genet, 2008, 25(9-10):483-488.

［17］于莹.荧光辅助去核在延边黄牛体细胞核移植中的应用［D］.延吉:延边大学，2009.

［18］NEBBIA C. Selenium in veterinary medicine ［J］. Riv Zoot Vet, 1982, 10:246-278.

［19］HANSEN J C, DEGUCHI Y. Selenium and fertility in animals and man a review ［J］. Acta Vet Scand, 1996, 37(1):19-30.

［20］HARRISON J H, HANCOK D D, CONRAD H R. Vitamin E and selenium for reproduction of the dairy cow ［J］. J Dairy Sci, 1984, 67(1):123-132.

［21］BARRINGTON J W, LINDSAY P, JAMES D, et al. Selenium deficiency and miscarriage: a possible link? ［J］. Br J Obstet Gynaecol, 1996, 103(2):130-132.

［22］EUYBOV I Z, SHIRINOV N M, RZAEV R I. Effect of selenium on reproductive organs and fertility of animals ［J］. Trud Azerbaizh Nauch Inst Vet, 1983, 28:103-105.

［23］AITKEN R J, CLARKSON J S, HARGREAVE T B, et al. Analysis of the relationship between defective sperm function and the generation of reactive oxygen species in cases of oligozoospermia ［J］. J Androl, 1989, 10(3):214-220.

［24］MIYAZAKI T, SUEOKA K, DHARMARAJJAN A M, et al. Effect of inhibition of oxygen free radical on ovulation and progesterone production by the in vitro perfused rabbit ovary ［J］. J Reprod Fertil, 199, 91(1):207-212.

［25］CETICA P D, PINTOS L N, DALVIT G C, et al. Antioxidant enzyme activity and oxidative stress in bovine oocyte in vitro maturation ［J］. IUBMB Life, 2001, 51(1):57-64.

［26］KODAMAN P H, BEHRMAN H R. Endocrine-regulated and protein kinase C-dependent generation of superoxide by rat preovulatory follicles ［J］. Endocrinology, 2001, 142(2):687-693.

［27］COMBELLES C M H, GUPTA S, AGARWAL A. Could oxidative stress influence the in vitro maturation of oocytes? ［J］. Reprod Biomed Online, 2009, 18(6): 864-880.

［28］SAITO Y, YOSHIDA Y, AKAZAWA T, et al. Cell death caused by selenium deficiency and protective effect of antioxidants ［J］. J Biol Chem, 2003, 278(41):39428-39434.

［29］ORSI N M, LEESE H J. Protection against reactive oxygen species during mouse preimplantation embryo development: role of EDTA, oxygen tension, catalase, superoxide dismutase and pyruvate ［J］. Mol Reprod Dev, 2001, 59(1):44-53.

［30］IWATA H, AKAMATSU S, MINAMI N, et al. Allopurinol, an inhibitor of xanthine oxidase, improves the development of IVM/IVF bovine embryos (>4 cell) in vitro under certain culture conditions ［J］. Theriogenology, 1999, 51(3):613-622.

［31］DE MATOS D G, FURNUS C C. The importance of having high glutathione (GSH) level after bovine in vitro maturation on embryo development: effect of β-mercaptoethanol, cysteine and cystine［J］. Theriogenology, 2000, 53(3): 761-771.

［32］KRISHER R L, BAVISTER B D. Responses of oocytes and embryos to the culture environment ［J］. Theriogenology, 1998, 49(1):103-114.

［33］EBERT R, ULMER M, ZECK S, et al. Selenium supplementation restores the antioxidative capacity and prevents cell damage in bone marrow stromal cells in vitro ［J］. Stem Cells, 2006, 24(5):1226-1235.

［34］BASINI G, TAMANINI C. Selenium stimulates estradiol production in bovine granulosa cells: possible involvement of nitric oxide ［J］. Dome Anim Endo, 2000, 18(1):1-17.

［35］CAVILLA J L, KENNEDY C R, BYSKOV A C, et al. Human immature oocytes grow during culture for IVM ［J］. Hum Reprod, 2008, 23(1):37-45.

［36］SAITO Y, YOSHIDA Y, AKAZAWA T, et al. Cell death caused by selenium deficiency and protective effect of antioxidants［J］. J Bio Chem, 2003, 278(41): 39428-39434.

［37］YEO J E, KANG S K. Selenium effectively inhibits ROS-mediated apoptotic neural precursor cell death in vitro and in vivo in traumatic brain injury ［J］. Biochim Biophys Acta, 2007, 1772(11):1199-1210.

[1]	YAN Xiaochun, XI Haijiao, LI Jinquan, WANG Zhiying, SU Rui. Study on Estimates of Genomic Breeding Value of Fleece Traits in Inner Mongolia Cashmere Goats [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 120-128.
[2]	ZHANG Chenjian, LI Yinxia, DING Qiang, LIU Weijia, WANG Huili, HE Nan, WU Jiashun, CAO Shaoxian. Efficient Preparation of CRISPR/Cas9-mediated Goat SOCS2 Gene Edited Embryos [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 129-141.
[3]	MENG Qiuchi, LU Guangyu, CHEN Dingshuang, LIN Yaqiu, WANG Ruilong, ZHONG Chaosong, WANG Yong, LIU Wei, WANG Youli, LI Yanyan, LI Zhixiong. Goats GPR35 Gene Expression Characteristics Analysis and the Effect on Subcutaneous Fat Cell Differentiation [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 4993-5007.
[4]	HE Zhicheng, QIN Xiaochen, Lü Yongqiang, LI Xiujin, BIAN Huilong, LUO Jun, LI Cong. Multiple Stepwise Regression Analysis and Growth Curve Fitting of Body Measurement Traits in Saanen Dairy Goats [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 5301-5311.
[5]	ZHANG Renbao, ZHOU Donghui, ZHOU Lisheng, GAO Xiaoxiao, LIU Nan, HE Jianning. Analysis of Genetic Structure of Conservation Population in Jining Gray Goats Based on 70 K SNP Chip [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2836-2847.
[6]	SHAO Yuexin, ZHANG Xinyu, GE Liyan, SHI Huaiping. Cloning and RNA Interference Analysis of ATF4 Gene in Xinong Saanen Dairy Goat [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2353-2364.
[7]	LIU Jie, XU Xiangping, DENG Ming, ZOU Xian, JIANG Shengwei, LIU Dewu, LIU Guangbin, SUN Baoli, GUO Yongqing, LI Yaokun. Effect of miR-144-5p Targeting WNT5a on the Proliferation and Apoptosis of Goat Ovarian Granulosa Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2421-2435.
[8]	XU Tiantian, ZHANG Tongtong, WANG Meng, WANG Xin. Transcription Factor Foxq1 Affects the Proliferation of Hair Follicle Stem Cells in Cashmere Goats via WNT/β-catenin Signaling Pathway [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2653-2661.
[9]	QUBI Wuqie, LI Yanyan, LI Xin, WANG Yong, WANG Youli, LIU Wei, ZHU Jiangjiang, LIN Yaqiu. APOA4 Gene Inhibits Intramuscular Adipocyte Differentiation in Goat [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 1927-1938.
[10]	MA Keyan, HAN Jintao, BAI Yaqin, LI Taotao, MA Youji. Genetic Diversity Analysis of Yongdeng Qishan Sheep Based on Specific-Locus Amplified Fragment Sequencing [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 1939-1950.
[11]	WANG Li, GUO Yaru, ZHANG Junmei, LEI Mingkai, WANG Zhenguo, ZHANG Chunxiang, REN Youshe. Effect of 1,25(OH)₂D₃ and VDR Knockout on the Expression of β-defensins Family in the Caprine Epididymal Caput Epithelial Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 1990-2000.
[12]	ZHU Junru, WEI Wei, PEI Dangshuai, ZHANG Fenque, DUAN Yu, GUO Yongfeng, JIANG Yue, XIA Shuli, HAN Jing, HOU Jinxing, AN Xiaopeng. Effects of PDCD4 on the Apoptosis of Dairy Goat Mammary Epithelial Cells and the Synthesis of β-casein and TG [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(4): 1429-1440.
[13]	CHEN Cancan, JIANG Jing, SUN Xiaoyan, REN Hangxing, LI Jie. Expression of AGRP Gene in Goat Tissue and Its Action Mechanism on Melanin Production [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(4): 1441-1451.
[14]	DAN Yixin, YANG Lu, XIANG Hua, ZHANG Huanrong, REN Yupeng, YANG Falong, HE Honghong, ZHU Jiangjiang. Effects of BIRC5 on the Cycle and Apoptosis of Goat Testis Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(4): 1511-1524.
[15]	YE Junning, DENG Ming, XUE Huiwen, LIU Guangbin, ZOU Xian, SUN Baoli, GUO Yongqing, LIU Dewu, LI Yaokun. Identification and Analysis of mRNA and lncRNA Affecting Goat Fetal Muscle Development [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(3): 989-1002.

Effects of Sodium Selenite on the Yanbian Dairy Goat Oocytes in vitro Maturation and the Effects on the Development of Somatic Cell Nuclear Transfer Embryos

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments