山羊GHSR基因多态性及其与体重和体尺性状的相关性研究

doi:10.11843/j.issn.0366-6964.2013.11.004

Abstract

Abstract:

The aim of this study was to analyze the association of GHSR gene polymorphisms with body weight and body size traits in goat. Guizhou White goats and Qianbei Ma goats were selected as testing subjects, the DNA pooling was constructed, the direct sequencing technology of PCR products and PCR-SSCP were used to detect the single nucleotide polymorphism of GHSR gene. The result showed that G996A and T1424C were detected in exon 2 and 3′UTR but no SNPs in exon1 and 5′UTR of GHSR gene in these goat breeds, respectively. The G996A was a sense mutation and devided into three genotypes:GG, GA and AA. The study of relationships between genotypes and body weight and body size traits revealed that body weight of individuals of Qianbei Ma goats with genotype GG and GA were significantly higher than those with genotype AA, and chest depth was smaller than those with genotype AA(P<0.05), no significant association of G996A with other indexes was found (P>0.05). Otherwise, the individuals with GG genotype had significantly better body weight, body length and hucklebone width than individuals with genotype AA (P<0.05) in Guizhou White goats. The result indicated that GHSR gene maybe a potential major gene or link to major gene effecting goat body weight, and G996A maybe a candidate molecular marker of MAS.

CLC Number:

S827
S813.3

SONG Tao-wei, LUO Wei-xing, CAI Hui-fen, LIU Bin, SUN Yan-yan. Polymorphism of GHSR Gene and Its Relationship with Body Weight and Body Size Traits in Goat[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(11): 1717-1723.

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Polymorphism of GHSR Gene and Its Relationship with Body Weight and Body Size Traits in Goat

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