辽宁新品系绒山羊GMPR2基因的生物学功能研究

doi:10.11843/j.issn.0366-6964.2013.11.003

Abstract

Abstract:

This study aimed to investigate the function of GMPR2 gene which was found in the skin of Liaoning New-breeding Cashmere goat and its relationship with the cashmere fiber growth. Bioinformatics analysis was firstly used, and then positioning analysis was got by using DIG-labeling in situ hybridization technique, and semi-quantitative real-time PCR was used to detect the levels of GMPR2 gene expression in different periods, and then, the RT-PCR was used to detect whether GMPR2 gene was expressed in other organs. Finally, gene expression was affected by different concentration melatonin treatment at different time. The results showed that the gene was really GMPR2 gene, which showed high homology to the Bos taurus GMPR2 gene. The in situ hybridization result showed that GMPR2 had a strong signal in inner root sheath of primary and secondary follicles. Besides, the expression of GMPR2 genes in primary follicles during catagen was the highest (P<0.01), but the expression was not obviously different between anagen and telogen. In addition, RT-PCR analysis showed that GMPR2 gene was only expressed in goat skin, not in liver and heart.The expression quantity of GMPR2 was decreased after melatonin treatment. It was infered that GMPR2 may indirectly regulate the primary and secondry follicles stunted growth.

CLC Number:

S827
S813.1

JIN Mei, ZHANG Li-li, QU Yang-le, PIAO Jun, LI Xiu-na, WANG Yi-meng, PIAO Jing-ai. Study on Biological Function of GMPR2 Gene in Liaoning New-breeding Cashmere Goat[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(11): 1706-1716.

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Study on Biological Function of GMPR2 Gene in Liaoning New-breeding Cashmere Goat

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