猪MSTN基因的多态性和生长性状关联分析

doi:10.11843/j.issn.0366-6964.2013.07.011

Abstract

Abstract:

Myostatin gene (MSTN) was regarded as a candidate gene for selection of porcine growth trait. In this study, the SNPs of MSTN gene had been detected and its association with porcine growth had been analyzed based on reports on its gene structure and function to put it as molecular genetic markers in porcine breeding. Using templates of genome DNA from 6 breeds including Landrace, Yorkshire, Duroc×(Landrace×Large White), Tongcheng pig, Wuzhishan pig, Laiwu pig, the SNPs were identified by cloning and sequencing of MSTN gene located in its promoter region, intron1, intron2, exon1, exon2 and 3′UTR. Matrix assisted laser desorption ionization time-of-flight mass spectrometry methed was used to genotype SNPs in MSTN gene in Landrace and Duroc×(Landrace×Large white) two experimental group and the data was assessdzed by SAS software. Total 16 SNPs were screened from 76 individuals of 6 breeds, of which 4 located in the promoter region, 5 located intron1, 7 located in intron2, while no SNPs was discovered in exon1, exon2 and its 3′UTR region. Association analysis of four SNPs (P1、P3 and P4 located in the promoter region; P5 located in intron1) in MSTN gene with growth traits indicated that P4 and P5 were significantly correlated (P<0.05) with growth traits. Both P4 and P5 have the potential application value as molecular marker in porcine breeding

CLC Number:

S828.2

LIU Xiao-qin, MA Xi-shan, TANG Zhong-lin, ZHOU Rong, YANG Shu-lin,AO Hong, MU Yu-lian, LI Kui. Polymorphism of MSTN Gene and Its Association with Growth Traits in Porcine[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(7): 1063-1069.

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Polymorphism of MSTN Gene and Its Association with Growth Traits in Porcine

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