陈皮精油对脂多糖诱导的RAW264.7细胞炎症的干预作用

doi:10.11843/j.issn.0366-6964.2024.09.039

摘要/Abstract

摘要：

基于脂多糖(lipopolysaccharide, LPS)诱导的RAW264.7细胞模型研究陈皮精油抗炎作用。通过Griess法检测一氧化氮(nitric oxide, NO)分泌水平, 采用Real-time PCR法检测细胞中肿瘤坏死因子(tumor necrosis factor α, TNF-α)、白细胞介素6(interleukin-6, IL-6)、白细胞介素1β(interleukin-1β, IL-1β)和诱导型一氧化氮合酶(inducible nitric oxide synthase, iNOS)、环氧化酶2(cyclooxygenase 2, COX-2) mRNA的表达水平。结果显示, 陈皮精油显著降低LPS诱导的RAW264.7细胞释放NO的含量, 抑制iNOS、COX-2、IL-1β、IL-6和TNF-α mRNA的表达。综上所述, 陈皮精油通过抑制炎症因子的分泌和表达, 有效缓解由LPS诱导RAW264.7细胞炎症反应。

关键词: 陈皮, 精油, 抗炎, LPS, RAW264.7细胞

Abstract:

The anti-inflammatory effect of essential oils from Citrus reticulata Blanco was investigated based on lipopolysaccharide (LPS)-induced RAW264.7 cell model. The level of Nitric oxide (NO) secretion was detected by Griess method, and the mRNA expression levels of tumor necrosis factor (TNF-α), interleukin-6 (IL-6), interleukin-1β (IL-1β), inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX-2) were determined by real-time PCR method. The results showed that essential oils from Citrus reticulata Blanco significantly decreased the level of NO released from LPS-induced RAW264.7 cells and inhibited the expression of iNOS, COX-2, IL-1β, IL-6 and TNF-α mRNA. In conclusion, essential oils from Citrus reticulata Blanco can inhibit the secretion and expression of inflammatory factors, and thus alleviate the inflammatory response induced by LPS in RAW264.7 cells effectively.

Key words: Citrus reticulata Blanco, essential oils, anti-inflammatory, LPS, RAW264.7 cell

中图分类号:

S853.7

刘玲, 史万玉, 李秀梅, 王明华, 翟向和, 周炜炜. 陈皮精油对脂多糖诱导的RAW264.7细胞炎症的干预作用[J]. 畜牧兽医学报, 2024, 55(9): 4153-4160.

Ling LIU, Wanyu SHI, Xiumei LI, Minghua WANG, Xianghe ZHAI, Weiwei ZHOU. Intervention of Essential Oils from Citrus reticulata Blanco on Lipopolysaccharide- induced Inflammation in RAW264.7 Cells[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(9): 4153-4160.

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