宏基因组技术诊断亲内皮疱疹病毒感染致濒危亚洲象死亡病例

doi:10.11843/j.issn.0366-6964.2024.06.028

畜牧兽医学报 ›› 2024, Vol. 55 ›› Issue (6): 2569-2577.doi: 10.11843/j.issn.0366-6964.2024.06.028

宏基因组技术诊断亲内皮疱疹病毒感染致濒危亚洲象死亡病例

沈青春¹(), 仉伟²(), 刘青²(), 刘一朵³, 钱佳豪¹, 张博源¹, 周师众¹, 张宜宸¹, 幸倩如¹, 丁家波¹, 周向梅³, 张广智¹, 李秋辰¹, 李伟²^,*(), 范学政¹^,*()

1. 中国农业科学院北京畜牧兽医研究所, 北京 100193
2. 济南市公园发展服务中心, 济南 250099
3. 中国农业大学动物医学院, 北京 100193

收稿日期:2023-09-18 出版日期:2024-06-23 发布日期:2024-06-28
通讯作者: 李伟,范学政 E-mail:shenqingchun@caas.cn;13176688152@163.com;liuqing0631@163.com;1279257279@qq.com;fanxuezheng@caas.cn
作者简介:沈青春(1976-)，男，湖北麻城人，正高级兽医师/研究员，博士，主要从事动物支原体、布鲁氏菌病流行病学与防控技术及生物信息学研究，E-mail: shenqingchun@caas.cn
仉伟(1979-)，山东泰安人，高级兽医师，主要从事野生动物疾病防治工作，E-mail：13176688152@163.com
刘青(1983-)，山东威海人，高级兽医师，博士，主要从事野生动物疾病检测工作，E-mail: liuqing0631@163.com
第一联系人：
沈青春、仉伟和刘青为同等贡献作者
基金资助:
重大外来动物疫病阻断与防控技术研发项目(2022YFD1800500)

Diagnosis of Death Cases of an Endangered Asian Elephants Caused by Endothelial Herpesvirus Infection with Metagenomic Technology

Qingchun SHEN¹(), Wei ZHANG²(), Qing LIU²(), Yiduo LIU³, Jiahao QIAN¹, Boyuan ZHANG¹, Shizhong ZHOU¹, Yichen ZHANG¹, Qianru XING¹, Jiabo DING¹, Xiangmei ZHOU³, Guangzhi ZHANG¹, Qiuchen LI¹, Wei LI²^,*(), Xuezheng FAN¹^,*()

1. Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China
2. Jinan Park Development Service Center, Jinan 250099, China
3. College of Veterinary Medicine, China Agricultural University, Beijing 100193, China

Received:2023-09-18 Online:2024-06-23 Published:2024-06-28
Contact: Wei LI, Xuezheng FAN E-mail:shenqingchun@caas.cn;13176688152@163.com;liuqing0631@163.com;1279257279@qq.com;fanxuezheng@caas.cn

摘要/Abstract

摘要：

本研究旨在借助宏基因组测序分析技术快速发现引起濒危亚洲象死亡的可能病原，再结合常规诊断技术，对大象病因进行分析和确诊，为大象相关疫病的快速诊断和预防提供参考。对死亡大象进行尸检，取心、肝、脾、肺、肾、淋巴结、肌肉、胃、十二指肠、空肠、结肠、盲肠、胰腺等器官制备切片，进行HE染色和病理分析；取腹股沟淋巴结进行宏基因组测序分析；使用特异性PCR方法对特征基因进行扩增和测序，经序列比对绘制进化树，分析其与其他毒株的进化关系。结果显示，死亡大象的剖检和病例切片结果符合象亲内皮疱疹病毒感染的特征，宏基因组分析结果显示，大象的腹股沟淋巴结中含有大量的象β疱疹病毒1型(即亲内皮疱疹病毒)。该大象死亡的原因可能是象亲内皮疱疹病毒感染所致，宏基因组技术对大象亲内皮疱疹病毒感染以及其他濒危动物病例的快速诊断具有良好的应用前景。

关键词: 亚洲象, 亲内皮疱疹病毒, 宏基因组分析

Abstract:

This study aims to use metagenomic sequencing analysis technology to quickly diagnose the possible pathogens causing the death of an Asian elephant, and then combine with conventional diagnostic techniques to analyze and confirm the etiological cause of elephant diseases, so as to provide reference for rapid diagnosis and prevention of elephant-related diseases. Autopsies were performed on dead elephant, and organs such as the heart, liver, spleen, lungs, kidneys, lymph nodes, muscles, stomach, duodenum, jejunum, colon, cecum, and pancreas were collected and sectioned for HE staining and pathological analysis; Metagenomic sequencing analysis was performed on the inguinal lymph nodes; species-specific PCR were used to amplify and sequence the characteristic genes, and an evolutionary tree was drawn to analyze its phylogenetic relationship with other strains. Data showed that necropsy and case biopsy results of dead elephant are consistent with the clinical characteristics of endothelial herpesvirus infection in elephants. Metagenomic analysis detected a large number of elephant betaherpesvirus type 1 (i.e., parent endothelial herpes virus) in the inguinal lymph nodes of elephants. The death of the elephant was probably caused by endothelial herpesvirus infection. Metagenomic technology offers valuable application prospects for the rapid diagnosis of endothelial herpesvirus infection in elephants as well as other endangered animal clinical infectious cases.

Key words: Asian elephant, endothelial herpesvirus, metagenomic analysis

中图分类号:

S858.93

沈青春, 仉伟, 刘青, 刘一朵, 钱佳豪, 张博源, 周师众, 张宜宸, 幸倩如, 丁家波, 周向梅, 张广智, 李秋辰, 李伟, 范学政. 宏基因组技术诊断亲内皮疱疹病毒感染致濒危亚洲象死亡病例[J]. 畜牧兽医学报, 2024, 55(6): 2569-2577.

Qingchun SHEN, Wei ZHANG, Qing LIU, Yiduo LIU, Jiahao QIAN, Boyuan ZHANG, Shizhong ZHOU, Yichen ZHANG, Qianru XING, Jiabo DING, Xiangmei ZHOU, Guangzhi ZHANG, Qiuchen LI, Wei LI, Xuezheng FAN. Diagnosis of Death Cases of an Endangered Asian Elephants Caused by Endothelial Herpesvirus Infection with Metagenomic Technology[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(6): 2569-2577.

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宏基因组技术诊断亲内皮疱疹病毒感染致濒危亚洲象死亡病例

Diagnosis of Death Cases of an Endangered Asian Elephants Caused by Endothelial Herpesvirus Infection with Metagenomic Technology

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