奶牛产后急性子宫内膜炎血液氧化脂质组变化特征

doi:10.11843/j.issn.0366-6964.2024.01.035

摘要/Abstract

摘要： 从血液氧化脂质组的变化特征角度阐明奶牛产后急性子宫内膜炎的发病机制。在宁夏某一集约化大型奶牛场，选取体况评分和胎次相近的产后7 d内患有子宫内膜炎的荷斯坦奶牛7头，治疗前视为试验组（E），治疗恢复后视为对照组（C）。两组奶牛于晨饲前采集血液，制备血浆，采用高效液相色谱-串联质谱（UPLC-MS/MS）联用技术定量检测两组血浆中氧化脂质的含量，并采用脂质组学技术分析两组血浆氧化脂质代谢轮廓的变化，筛选差异氧化脂质进行代谢通路富集分析。结果显示：E组血浆代谢轮廓较C组发生显著变化，10种氧化脂质发生了显著变化（P<0.05，VIP≥1，FC≥2或FC≤0.5），其中4种上调，包括12-HEPE（羟基二十碳五烯酸）、5-HEPE、12-HETE（羟基二十碳四烯酸）、14（S）-HDHA（羟基二十二碳六烯酸），6种下调，包括9，10-EpOME（环氧十八碳烯酸）、12，13-EpOME、11，12-EET（环氧二十碳三烯酸）、8，9-EET、14，15-EET、17（18）-EpETE（环氧二十碳四烯酸）；差异代谢物富集在花生四烯酸代谢、TRP通道的炎症介质调节、PPAR信号通路和血管平滑肌收缩通路。奶牛产后急性子宫内膜炎的发生与体内氧化脂质的变化密切相关，这些氧化脂质可能通过花生四烯酸代谢通路、TRP通道的炎症介质调节、PPAR信号通路和血管平滑肌收缩等代谢通路参与了产后急性子宫内膜炎的发生与发展。

关键词: 产后急性子宫内膜炎, 氧化脂质, 差异代谢物, 代谢通路, 发病机制

Abstract: The pathogenesis of acute Endometritis in postpartum dairy cows was elucidated from the change characteristics of blood lipid oxide group. Seven Holstein dairy cows with endometritis within 7 days after delivery, which were similar body condition scoring and tire postpartum parity, were selected from a large-scale intensive dairy farm in Ningxia. Before treatment, they were regarded as test group (E), and after treatment, they were regarded as control group (C). Blood samples were collected before morning feeding and plasma was prepared. The content of oxidized lipids in plasma was quantitatively detected by high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The changes of plasma oxidized lipid metabolic profiles were analyzed by lipomics technology, and the differentially oxidized lipid metabolites were screened for metabolic pathway enrichment analysis. The plasma metabolic profile of E group was significantly different from that of C group (P<0.05, VIP≥1, FC ≥2 or FC≤0.5), of which 4 were up-regulated, including 12-HEPE(hydroxyeicosapentaenoic acid), 5-HEPE, 12-HETE(hydroxyeicosatetraenoic acid),14(S)-HDHA(hydroxydocosahexaenoic acid), and 6 were down-regulated, including 9,10-EpOME(epoxyoctadecenoic acid),12,13-EpOME,11,12-EET(epoxyeicosatrienoic acid),8,9-EET,14,15-EET and 17(18)-EpETE(epoxyeicosatetraenoic acid); The differential metabolites were enriched in arachidonic acid metabolism, inflammatory mediator regulation of TRP channel, PPAR signaling pathway, vascular smooth muscle contraction. The occurrence of acute endometritis in postpartum dairy cows is closely related to the changes of oxidized lipids in vivo. These oxidized lipids may participate in the occurrence and development of postpartum acute endometritis through arachidonic acid metabolic pathway, inflammatory mediator regulation of TRP channel, PPAR signaling pathway and vascular smooth muscle contraction.

Key words: endometritis, oxidized lipid, differential metabolites, metabolic pathway, pathogenesis

中图分类号:

S857.23

王瑞玲, 王雪妍, 王菲菲, 孔维怡, 毛永霞, 刘欣, 丁辉, 许立华, 郭延生. 奶牛产后急性子宫内膜炎血液氧化脂质组变化特征[J]. 畜牧兽医学报, 2024, 55(1): 373-387.

WANG Ruiling, WANG Xueyan, WANG Feifei, KONG Weiyi, MAO Yongxia, LIU Xin, DING Hui, XU Lihua, GUO Yansheng. Study on the Changes of Blood Oxidized Lipid Group in Postpartum Dairy Cows with Acute Endometritis[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 373-387.

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