动物肠缺氧模型研究进展

doi:10.11843/j.issn.0366-6964.2023.10.008

畜牧兽医学报 ›› 2023, Vol. 54 ›› Issue (10): 4083-4094.doi: 10.11843/j.issn.0366-6964.2023.10.008

动物肠缺氧模型研究进展

戴文¹, 卞苏舒^1,2, 张聚民², 宋厚辉¹, 周莹珊¹, 刘萍^1*, 王晓杜^1*

1. 浙江农林大学动物科技学院·动物医学院浙江省畜禽绿色生态健康养殖应用技术研究重点实验室动物健康互联网检测技术浙江省工程研究中心浙江省动物医学与健康管理国际科技合作基地中澳动物健康大数据分析联合实验室, 杭州 311300;
2. 杭州市临安区畜牧农机发展中心, 杭州 311300

收稿日期:2022-11-11 出版日期:2023-10-23 发布日期:2023-10-26
通讯作者: 王晓杜,主要从事动物病毒学研究,E-mail:xdwang@zafu.edu.cn;刘萍,主要从事中兽医学研究,E-mail:liupingvet@zafu.edu.cn
作者简介:戴文(1998-),女,浙江台州人,硕士生,主要从事肠道缺氧及其作用机制研究,E-mail:1157128733@qq.com
基金资助:
浙江省三农六方项目（2021SNLF023）；浙江省农业重大技术协同推广计划项目（2021XTTGXM02）；浙江农林大学科研发展基金项目（2021FR002）

Research Progress of Intestinal Hypoxia Models in Animals

DAI Wen¹, BIAN Sushu^1,2, ZHANG Jumin², SONG Houhui¹, ZHOU Yingshan¹, LIU Ping^1*, WANG Xiaodu^1*

1. China-Australia Joint Laboratory for Animal Health Big Data Analytics, Zhejiang International Science and Technology Cooperation Base for Veterinary Medicine and Health Management, Zhejiang Provincial Engineering Laboratory for Animal Health Inspection & Internet Technology, Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, College of Animal Science and Technology & College of Veterinary Medicine, Zhejiang A&F University, Hangzhou 311300, China;
2. Development Center of Animal Husbandry and Agricultural Machinery in Hangzhou Lin'an District, Hangzhou 311300, China

Received:2022-11-11 Online:2023-10-23 Published:2023-10-26

摘要/Abstract

摘要： 肠缺氧是肠组织氧气需求量高于供应量的病理现象，是多种肠道疾病的直接诱发因素、预警信号和关键特征之一。建立真实可靠的肠缺氧状态动物模型或者能模拟肠缺氧的细胞模型，对肠缺氧相关疾病的病理机制研究至关重要。本文综述了近年来适用于构建肠缺氧模型的方法，重点围绕循环性、化学性和环境性肠缺氧动物模型，物理法和化学法诱导的离体肠细胞缺氧模型和离体肠道类器官缺氧模型的构建策略、模型优劣及应用场景进行分析，为动物肠缺氧疾病的发病机制研究及治疗药物的发掘和临床药效学评价提供参考。

关键词: 肠缺氧, 细胞模型, 动物模型, 肠道类器官

Abstract: Intestinal hypoxia is a pathological phenomenon in which the oxygen demand of intestinal tissues is higher than the oxygen supply. It is one of the direct triggers, early warning signals and key features of various intestinal diseases. The establishment of real and reliable animal models of intestinal hypoxia or cellular models that can simulate intestinal hypoxia is essential for the study of the pathological mechanisms of intestinal hypoxia-related diseases. The methods applicable to the construction of intestinal hypoxia models in recent years are reviewed in this paper, Focused on the construction strategies, advantages and disadvantages, and application scenarios of models including circulatory, chemical and environmental animal models of intestinal hypoxia, physically and chemically induced cellular models of intestinal hypoxia in vitro, as well as isolated intestinal organoid models of hypoxia in vitro, in the hope of providing reference for the study of the pathogenesis of intestinal hypoxia diseases in animals and the discovery and clinical pharmacodynamic evaluation of therapeutic drugs.

Key words: intestinal hypoxia, cellular models, animal models, intestinal organoids

中图分类号:

S858

戴文, 卞苏舒, 张聚民, 宋厚辉, 周莹珊, 刘萍, 王晓杜. 动物肠缺氧模型研究进展[J]. 畜牧兽医学报, 2023, 54(10): 4083-4094.

DAI Wen, BIAN Sushu, ZHANG Jumin, SONG Houhui, ZHOU Yingshan, LIU Ping, WANG Xiaodu. Research Progress of Intestinal Hypoxia Models in Animals[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(10): 4083-4094.

参考文献 89

[1]	COLGAN S P, TAYLOR C T.Hypoxia:an alarm signal during intestinal inflammation[J].Nat Rev Gastroenterol Hepatol, 2010, 7(5):281-287.
[2]	ELTZSCHIG H K, ECKLE T.Ischemia and reperfusion-from mechanism to translation[J].Nat Med, 2011, 17(11):1391-1401.
[3]	马殿伟, 谢学军, 李晓微.缺氧实验模型研究进展[J].医学综述, 2007, 13(23):1795-1798.MA D W, XIE X J, LI X W.Research development of experimental model with hypoxia[J].Medical Recapitulate, 2007, 13(23):1795-1798.(in Chinese)
[4]	TAYLOR C T, COLGAN S P.Regulation of immunity and inflammation by hypoxia in immunological niches[J].Nat Rev Immunol, 2017, 17(12):774-785.
[5]	VAN WELDEN S, SELFRIDGE A C, HINDRYCKX P.Intestinal hypoxia and hypoxia-induced signalling as therapeutic targets for IBD[J].Nat Rev Gastroenterol Hepatol, 2017, 14(10):596-611.
[6]	ROBRAHN L, JIAO L, CRAMER T.Barrier integrity and chronic inflammation mediated by HIF-1 impact on intestinal tumorigenesis[J].Cancer Lett, 2020, 490:186-192.
[7]	TAYLOR C T, COLGAN S P.Hypoxia and gastrointestinal disease[J].J Mol Med (Berl), 2007, 85(12):1295-1300.
[8]	PRAL L P, FACHI J L, CORRÊA R O, et al.Hypoxia and HIF-1 as key regulators of gut microbiota and host interactions[J].Trends Immunol, 2021, 42(7):604-621.
[9]	温爽.复方中药对腹水综合征肉鸡空肠紧密连接和炎症因子的影响[D].晋中:山西农业大学, 2019.WEN S.Effect of compound traditional Chinese medicine on jejunum tight junction and inflammatory factors in broilers with Ascitic Syndrome[D].Jinzhong:Shanxi Agricultural University, 2019.(in Chinese)
[10]	GLOVER L E, LEE J S, COLGAN S P.Oxygen metabolism and barrier regulation in the intestinal mucosa[J].J Clin Invest, 2016, 126(10):3680-3688.
[11]	陆逸, 蔡杰, 王迪铭, 等.动物低氧应激与线粒体功能机制[J].动物营养学报, 2020, 32(11):5046-5052.LU Y, CAI J, WANG D M, et al.Hypoxic stress and mitochondrial function mechanism in animals[J].Chinese Journal of Animal Nutrition, 2020, 32(11):5046-5052.(in Chinese)
[12]	GRANGER D N, HOLM L, KVIETYS P.The gastrointestinal circulation:physiology and pathophysiology[J].Compr Physiol, 2015, 5(3):1541-1583.
[13]	杨硕菲, 吴性江.缺氧诱导因子在肠缺氧中的研究进展[J].肠外与肠内营养, 2015, 22(5):307-311.YANG S F, WU X J.Research advancement of hypoxia-inducible factor in intestinal hypoxia[J].Parenteral & Enteral Nutrition, 2015, 22(5):307-311.(in Chinese)
[14]	WAN Z Q, ZHANG X F, JIA X H, et al.Lactobacillus johnsonii YH1136 plays a protective role against endogenous pathogenic bacteria induced intestinal dysfunction by reconstructing gut microbiota in mice exposed at high altitude[J].Front Immunol, 2022, 13:1007737.
[15]	GONZALEZ L M, MOESER A J, BLIKSLAGER A T.Animal models of ischemia-reperfusion-induced intestinal injury:progress and promise for translational research[J].Am J Physiol Gastrointest Liver Physiol, 2015, 308(2):G63-G75.
[16]	DENG F, HU J J, YANG X, et al.Gut microbial metabolite pravastatin attenuates intestinal ischemia/reperfusion injury through promoting IL-33 release from type Ⅱ innate lymphoid cells via IL-33/ST2 signaling[J].Front Immunol, 2021, 12:704836.
[17]	GUO J F, LOU X K, GONG W Y, et al.The effects of different stress on intestinal mucosal barrier and intestinal microecology were discussed based on three typical animal models[J].Front Cell Infect Microbiol, 2022, 12:953474.
[18]	DENG F, LIN Z B, SUN Q S, et al.The role of intestinal microbiota and its metabolites in intestinal and extraintestinal organ injury induced by intestinal ischemia reperfusion injury[J].Int J Biol Sci, 2022, 18(10):3981-3992.
[19]	DENG F, ZHAO B C, YANG X, et al.The gut microbiota metabolite capsiate promotes Gpx4 expression by activating TRPV1 to inhibit intestinal ischemia reperfusion-induced ferroptosis[J].Gut Microbes, 2021, 13(1):1902719.
[20]	TIAN Y, SHU R, LEI Y, et al.Somatostatin attenuates intestinal epithelial barrier injury during acute intestinal ischemia-reperfusion through Tollip/Myeloiddifferentiationfactor 88/Nuclear factor kappa-B signaling[J].Bioengineered, 2022, 13(3):5005-5020.
[21]	MURTHY S, QING H Q, SAKAI T, et al.Ischemia/reperfusion injury in the rat colon[J].Inflammation, 1997, 21(2):173-190.
[22]	JI A L, LI T, ZU G, et al.Ubiquitin-specific protease 22 enhances intestinal cell proliferation and tissue regeneration after intestinal ischemia reperfusion injury[J].World J Gastroenterol, 2019, 25(7):824-836.
[23]	GAO Y F, ZHANG H J, WANG Y J, et al.L-cysteine alleviates myenteric neuron injury induced by intestinal ischemia/reperfusion via inhibitin the macrophage NLRP3-IL-1β pathway[J].Front Pharmacol, 2022, 13:899169.
[24]	LIU C L, DING R W, HUANG W J, et al.Sevoflurane protects against intestinal ischemia-reperfusion injury by activating peroxisome proliferator-activated receptor gamma/nuclear factor-κB pathway in rats[J].Pharmacology, 2020, 105(3-4):231-242.
[25]	PARLAR A, ARSLAN S O.Resveratrol normalizes the deterioration of smooth muscle contractility after intestinal ischemia and reperfusion in rats associated with an antioxidative effect and modulating tumor necrosis factor alpha activity[J].Ann Vasc Surg, 2019, 61:416-426.
[26]	LIU C L, SHEN Z W, LIU Y H, et al.Sevoflurane protects against intestinal ischemia-reperfusion injury partly by phosphatidylinositol 3 kinases/Akt pathway in rats[J].Surgery, 2015, 157(5):924-933.
[27]	TURAN I, OZACMAK H S, OZACMAK V H, et al.Agmatine attenuates intestinal ischemia and reperfusion injury by reducing oxidative stress and inflammatory reaction in rats[J].Life Sci, 2017, 189:23-28.
[28]	SAYHAN M B, OGUZ S, SALT Ö, et al.Sesamin ameliorates mucosal tissue injury of mesenteric ischemia and reperfusion in an experimental rat model[J].Arch Med Sci, 2019, 15(6):1582-1588.
[29]	ANSARI F A, KHAN A A, MAHMOOD R.Protective effect of carnosine and N-acetylcysteine against sodium nitrite-induced oxidative stress and DNA damage in rat intestine[J].Environ Sci Pollut Res Int, 2018, 25(20):19380-19392.
[30]	KHATUN F, AIZU Y, NISHIDATE I.Transcutaneous monitoring of hemoglobin derivatives during methemoglobinemia in rats using spectral diffuse reflectance[J].J Biomed Opt, 2021, 26(3):033708.
[31]	BAI X, LIU G Q, YANG J X, et al.Changes in the gut microbiota of rats in high-altitude hypoxic environments[J].Microbiol Spectr, 2022, 10(6):e01626-22.
[32]	罗涵.TLR4/NF-κB在低氧暴露下大鼠肠道屏障功能损伤及细菌移位中的作用研究[D].重庆:第三军医大学, 2013.LUO H.Role of TLR4/NF-κB in damage to intestinal mucosa barrier function and bacterial translocation in rats exposed to hypoxia[D].Chongqing:Third Military Medical University, 2013.(in Chinese)
[33]	JI Q R, ZHANG Y, ZHOU Y L, et al.Effects of hypoxic exposure on immune responses of intestinal mucosa to Citrobacter colitis in mice[J].Biomed Pharmacother, 2020, 129:110477.
[34]	赫玉宝.模拟急进高原缺氧对大鼠肠黏膜的损伤及缺氧相关蛋白的影响[D].兰州:甘肃中医药大学, 2018.HE Y B.Effect of simulated acute plateau hypoxia on intestinal mucosa injury and hypoxia-related protein in rats[D].Lanzhou:Gansu University of Chinese Medicine, 2018.(in Chinese)
[35]	YIN L H, GAO M, XU L N, et al.Single-cell analysis of cellular heterogeneity and interactions in the ischemia-reperfusion injured mouse intestine[J/OL].J Pharm Anal, 2023[2022-11-11]..https://www.sciencedirect.com/science/article/pii/S2095177923000059.
[36]	DAI D, DAI F D, CHEN J C, et al.Integrated multi-omics reveal important roles of gut contents in intestinal ischemia-reperfusion induced injuries in rats[J].Commun Biol, 2022, 5(1):938.
[37]	AHMED M.Ischemic bowel disease in 2021[J].World J Gastroenterol, 2021, 27(29):4746-4762.
[38]	余鸿, 吴雨岭, 韩艺, 等.缺氧条件下鼠胚软骨c-Fos和巢蛋白表达及当归的保护作用[J].中国临床康复, 2006, 10(11):111-113.YU H, WU Y L, HAN Y, et al.Effect of Chinese angelica on the expression of c-Fos and nidogen in embryonic cartilage of rats during hypoxia stage[J].Chinese Journal of Clinical Rehabilitation, 2006, 10(11):111-113.(in Chinese)
[39]	CHENG J F, SUN Y M, HE J X, et al.The mechanism of colon tissue damage mediated by HIF-1α/NF-κB/STAT1 in high-altitude environment[J].Front Physiol, 2022, 13:933659.
[40]	王黎, 史清海, 胡科妍, 等.优化及复氧对急进高原缺氧大鼠认知功能障碍的改善作用[J].华南国防医学杂志, 2018, 32(1):5-7, 41.WANG L, SHI Q H, HU K Y, et al.Improvement of optimization and reoxygenation on cognitive dysfunction in plateau hypoxia rats[J].Military Medical Journal of South China, 2018, 32(1):5-7, 41.(in Chinese)
[41]	BURTSCHER J, MALLET R T, BURTSCHER M, et al.Hypoxia and brain aging:neurodegeneration or neuroprotection?[J].Ageing Res Rev, 2021, 68:101343.
[42]	RIECH S, KALLENBERG K, MOERER O, et al.The pattern of brain microhemorrhages after severe lung failure resembles the one seen in high-altitude cerebral edema[J].Crit Care Med, 2015, 43(9):e386-e389.
[43]	李龙, 刘锁珠, 王宏辉, 等.益生菌对高海拔缺氧条件下肉鸡生产性能和肠道功能的影响[J].家畜生态学报, 2018, 39(7):25-29.LI L, LIU S Z, WANG H H, et al.Effects of probiotics on growth performance and intestine function of broiler reared at hypoxic environment in high altitude[J].Acta Ecologiae Animalis Domastici, 2018, 39(7):25-29.(in Chinese)
[44]	滕文彬, 李玉红, 祝胜美.低氧诱导因子的调控途径和在肠道疾病中的作用[J].中国病理生理杂志, 2019, 35(10):1894-1900.TENG W B, LI Y H, ZHU S M.Regulatory pathway of hypoxia-inducible factor and its role in intestinal diseases[J].Chinese Journal of Pathophysiology, 2019, 35(10):1894-1900.(in Chinese)
[45]	马雪, 史清海.缺氧动物及细胞实验模型的研究进展[J].西北国防医学杂志, 2016, 37(8):535-538.MA X, SHI Q H.Research progress of hypoxic animal and cell experimental models[J].Medical Journal of National Defending Forces in Northwest China, 2016, 37(8):535-538.(in Chinese)
[46]	杨松巍.NF-κB/HIF-1α通路在IFN-γ诱导的肠上皮屏障损害中的机制研究[D].重庆:第三军医大学, 2014.YANG S W.NF-κB/HIF-1α pathway involved in IFN-γ induced intestinal epithelial barrier disfunction[D].Chongqing:Third Military Medical University, 2014.(in Chinese)
[47]	王彬彬.模拟高原缺氧大鼠肠上皮细胞损伤自噬调控机制的研究[D].兰州:甘肃中医药大学, 2016.WANG B B.Research mechanism of autophagy in rat intestinal epithelial cells injury induced by simulated altitude hypoxia[D].Lanzhou:Gansu University of Chinese Medicine, 2016.(in Chinese)
[48]	GAO H N, REN F Z, WEN P C, et al.Yak milk-derived exosomal microRNAs regulate intestinal epithelial cells on proliferation in hypoxic environment[J].J Dairy Sci, 2021, 104(2):1291-1303.
[49]	张秀杨, 张龙飞, 陈世远, 等.缺氧诱导因子1α介导单羧酸转运蛋白1表达参与短链脂肪酸对肠道缺氧保护作用的研究[J].中华普通外科学文献, 2023, 17(1):18-23.ZHANG X Y, ZHANG L F, CHEN S Y, et al.Hypoxia-inducible factor-1α mediating the expression of monocarboxylate transporter-1 and participating in the protective effect of short-chain fatty acids on intestinal hypoxia[J].Chinese Archives of General Surgery (Electronic Edition), 2023, 17(1):18-23.(in Chinese)
[50]	MUENCHAU S, DEUTSCH R, DE CASTRO I J, et al.Hypoxic environment promotes barrier formation in human intestinal epithelial cells through regulation of microRNA 320a expression[J].Mol Cell Biol, 2019, 39(14):e00553-18.
[51]	LI Y, FENG D C, WANG Z Y, et al.Ischemia-induced ACSL4 activation contributes to ferroptosis-mediated tissue injury in intestinal ischemia/reperfusion[J].Cell Death Differ, 2019, 26(11):2284-2299.
[52]	FACHI J L, PRAL L P, DOS SANTOS J A C, et al.Hypoxia enhances ILC3 responses through HIF-1α-dependent mechanism[J].Mucosal Immunol, 2021, 14(4):828-841.
[53]	XIE L W, COLLINS J F.Transcription factors Sp1 and Hif2α mediate induction of the copper-transporting ATPase (Atp7a) gene in intestinal epithelial cells during hypoxia[J].J Biol Chem, 2013, 288(33):23943-23952.
[54]	罗红敏.丙戊酸钠对严重烫伤后肠屏障功能的保护作用及机制研究[D].北京:中国人民解放军医学院, 2014.LUO H M.The protective effects of valproic acid on gut barrier function after major burn injury and its underlying mechanisms[D].Beijing:People's Liberation Army Medical College, 2014.(in Chinese)
[55]	BASAVARAJU A M, SHIVANNA N, YADAVALLI C, et al.Ameliorative effect of Ananas comosus on cobalt chloride-induced hypoxia in Caco2 cells via HIF-1α, GLUT 1, VEGF, ANG and FGF[J].Biol Trace Elem Res, 2021, 199(4):1345-1355.
[56]	BONCLER M, LUKASIAK M, DASTYCH J, et al.Differentiated mitochondrial function in mouse 3T3 fibroblasts and human epithelial or endothelial cells in response to chemical exposure[J].Basic Clin Pharmacol Toxicol, 2019, 124(2):199-210.
[57]	DIGUILIO K M, VALENZANO M C, RYBAKOVSKY E, et al.Cobalt chloride compromises transepithelial barrier properties of CaCo-2 BBe human gastrointestinal epithelial cell layers[J].BMC Gastroenterol, 2018, 18(1):2.
[58]	LIU Y L, WANG C H, WANG Y H, et al.Cobalt chloride decreases fibroblast growth factor-21 expression dependent on oxidative stress but not hypoxia-inducible factor in Caco-2 cells[J].Toxicol Appl Pharmacol, 2012, 264(2):212-221.
[59]	DONG B L, SONG W H, KONG X Q, et al.Visualizing cellular sodium hydrosulfite (Na2S2O4) using azo-based fluorescent probes with a high signal-to-noise ratio[J].J Mater Chem B, 2019, 7(5):730-733.
[60]	TIAN Y, LI Y F, WANG W X, et al.Novel strategy for validating the existence and mechanism of the "Gut-Liver Axis" in vivo by a hypoxia-sensitive NIR fluorescent probe[J].Anal Chem, 2020, 92(6):4244-4250.
[61]	KHONG T L, THAIRU N, LARSEN H, et al.Identification of the angiogenic gene signature induced by EGF and hypoxia in colorectal cancer[J].BMC Cancer, 2013, 13:518.
[62]	CUMMINS E P, SEEBALLUCK F, KEELY S J, et al.The hydroxylase inhibitor dimethyloxalylglycine is protective in a murine model of colitis[J].Gastroenterology, 2008, 134(1):156-165.e1.
[63]	DENGLER F, GÄBEL G.The fast lane of hypoxic adaptation:Glucose transport is modulated via a HIF-hydroxylase-AMPK-axis in jejunum epithelium[J].Int J Mol Sci, 2019, 20(20):4993.
[64]	ZEITOUNI N E, DERSCH P, NAIM H Y, et al.Hypoxia decreases invasin-mediated Yersinia enterocolitica internalization into Caco-2 cells[J].PLoS One, 2016, 11(1):e0146103.
[65]	KAMIYA T, KOWN A H, KANEMAKI T, et al.A simplified model of hypoxic injury in primary cultured rat hepatocytes[J].In Vitro Cell Dev Biol Anim, 1998, 34(2):131-137.
[66]	MUÑOZ-SÁNCHEZ J, CHÁNEZ-CÁRDENAS M E.The use of cobalt chloride as a chemical hypoxia model[J].J Appl Toxicol, 2019, 39(4):556-570.
[67]	许蜀闽, 王培勇, 马红英.连二亚硫酸钠在建立培养细胞的无氧环境中的应用[J].第三军医大学学报, 2005, 27(4):359-360.XU S M, WANG P Y, MA H Y.Preparation of hypoxic surroundings with sodium dihionite for cell culture[J].Acta Academiae Medicinae Militaris Tertiae, 2005, 27(4):359-360.(in Chinese)
[68]	SWEET R, PAUL A, ZASTRE J.Hypoxia induced upregulation and function of the thiamine transporter, SLC19A3 in a breast cancer cell line[J].Cancer Biol Ther, 2010, 10(11):1101-1111.
[69]	SABUI S, RAMAMOORTHY K, ROMERO J M, et al.Hypoxia inhibits colonic uptake of the microbiota-generated forms of vitamin B1 via HIF-1α-mediated transcriptional regulation of their transporters[J].J Biol Chem, 2022, 298(2):101562.
[70]	王修启, 谢文文, 周加义.农业动物肠道类器官研究进展[J].饲料工业, 2022, 43(18):1-7.WANG X Q, XIE W W, ZHOU J Y.Recent advances in intestinal organoids of farm animals[J].Feed Industry, 2022, 43(18):1-7.(in Chinese)
[71]	ZUR BRUEGGE T F, LIESE A, DONATH S, et al.Intestinal organoids in colitis research:focusing on variability and cryopreservation[J].Stem Cells Int, 2021, 2021:9041423.
[72]	BURCLAFF J, BLITON R J, BREAU K A, et al.A proximal-to-distal survey of healthy adult human small intestine and colon epithelium by single-cell transcriptomics[J].Cell Mol Gastroenterol Hepatol, 2022, 13(5):1554-1589.
[73]	WÖRSDÖRFER P, ERGVN S.The impact of oxygen availability and multilineage communication on organoid maturation[J].Antioxid Redox Signal, 2021, 35(3):217-233.
[74]	ZHAO D, FARNELL M B, KOGUT M H, et al.From crypts to enteroids:establishment and characterization of avian intestinal organoids[J].Poult Sci, 2022, 101(3):101642.
[75]	HILL D R, HUANG S, NAGY M S, et al.Bacterial colonization stimulates a complex physiological response in the immature human intestinal epithelium[J].eLife, 2017, 6:e29132.
[76]	KIP A M, SOONS Z, MOHREN R, et al.Proteomics analysis of human intestinal organoids during hypoxia and reoxygenation as a model to study ischemia-reperfusion injury[J].Cell Death Dis, 2021, 12(1):95.
[77]	KIP A M, GROOTJANS J, MANCA M, et al.Temporal transcript profiling identifies a role for unfolded protein stress in human gut ischemia-reperfusion injury[J].Cell Mol Gastroenterol Hepatol, 2022, 13(3):681-694.
[78]	DE LANGE I H, VAN GORP C, MASSY K R I, et al.Hypoxia-driven changes in a human intestinal organoid model and the protective effects of hydrolyzed whey[J].Nutrients, 2023, 15(2):393.
[79]	KOIKE Y, LI B, LEE C, et al.The intestinal injury caused by ischemia-reperfusion is attenuated by amniotic fluid stem cells via the release of tumor necrosis factor-stimulated gene 6 protein[J].FASEB J, 2020, 34(5):6824-6836.
[80]	WALAAS G A, GOPALAKRISHNAN S, BAKKE I, et al.Physiological hypoxia improves growth and functional differentiation of human intestinal epithelial organoids[J].Front Immunol, 2023, 14:1095812.
[81]	ALLWOOD M A, EDGETT B A, EADIE A L, et al.Moderate and severe hypoxia elicit divergent effects on cardiovascular function and physiological rhythms[M].J Physiol, 2018, 596(15):3391-3410.
[82]	王艺博, 王峰, 肖智勇, 等.大株红景天胶囊提高小鼠耐常压缺氧能力和抗疲劳作用[J].中国药理学与毒理学杂志, 2021, 35(6):420-426.WANG Y B, WANG F, XIAO Z Y, et al.Large plant Rhodiola capsules improve normobaric hypoxia tolerance and anti-fatigue action in mice[J].Chinese Journal of Pharmacology and Toxicology, 2021, 35(6):420-426.(in Chinese)
[83]	董晓敏, 孙笑语, 童学红, 等.机能学实验中常压与低压缺氧环境下小鼠耐受性的比较[J].继续医学教育, 2018, 32(12):72-73.DONG X M, SUN X Y, TONG X H, et al.Comparison of tolerance between hypobaric hypoxia and normobaric hypoxia in medical functional teaching experiment[J].Continuing Medical Education, 2018, 32(12):72-73.(in Chinese)
[84]	MORRIS N L, CANNON A R, LI X L, et al.Protective effects of PX478 on gut barrier in a mouse model of ethanol and burn injury[J].J Leukoc Biol, 2021, 109(6):1121-1130.
[85]	CHOUDHRY M A, BA Z F, RANA S N, et al.Alcohol ingestion before burn injury decreases splanchnic blood flow and oxygen delivery[J].Am J Physiol Heart Circ Physiol, 2005, 288(2):H716-H721.
[86]	WALLIN D J, TKAC I, STUCKER S, et al.Phlebotomy-induced anemia alters hippocampal neurochemistry in neonatal mice[J].Pediatr Res, 2015, 77(6):765-771.
[87]	ARTHUR C M, NALBANT D, FELDMAN H A, et al.Anemia induces gut inflammation and injury in an animal model of preterm infants[J].Transfusion, 2019, 59(4):1233-1245.
[88]	BALLS M.It's time to reconsider the principles of humane experimental technique[J].Altern Lab Anim, 2020, 48(1):40-46.
[89]	BEAUMONT M, BLANC F, CHERBUY C, et al.Intestinal organoids in farm animals[J].Vet Res, 2021, 52(1):33.

动物肠缺氧模型研究进展

Research Progress of Intestinal Hypoxia Models in Animals

RichHTML

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献 89

相关文章 10

编辑推荐

Metrics

本文评价

[1]	武文英, 夏青, 胡萌婕, 赵逸轩, 王琛, 张宇豪, 郝成武, 贺笋, 郭爱珍, 陈建国, 陈颖钰. 牛支原体兔体攻毒模型的建立[J]. 畜牧兽医学报, 2024, 55(3): 1268-1277.
[2]	罗承慧, 高江瑞, 陈俊威, 魏春洁, 韦双双, 裴业春. 尘螨诱导特应性皮炎小鼠模型和哮喘小鼠模型的构建[J]. 畜牧兽医学报, 2024, 55(3): 1257-1267.
[3]	李林, 宫彬彬, 王广力, 赵梅, 张源淑. 乙酸钠对油酸诱导的BRL-3A细胞脂肪变性的影响及机制分析[J]. 畜牧兽医学报, 2022, 53(12): 4450-4460.
[4]	臧江华, 安一娜, 王婧, 王科智, 杨静静, 高敏, 冯岚迪, 谭姝瑜, 胡艳欣, 董彦君. 鸡源A型产气荚膜梭菌致病性及药物疗效分析[J]. 畜牧兽医学报, 2022, 53(10): 3561-3569.
[5]	李佳锴, 戴鹏秀, 陈奕静, 张露文, 王璟璐, 张翊华. 链脲佐菌素和四氧嘧啶联合注射建立犬I型糖尿病模型[J]. 畜牧兽医学报, 2021, 52(10): 2960-2968.
[6]	王玮玮，刘瑞琪，吴勇延，杨严格，王勇胜，卿素珠. CRISPR/Cas9基因编辑系统研究进展及其在动物基因编辑研究中的应用[J]. 畜牧兽医学报, 2016, 47(7): 1299-1305.
[7]	王自力，贺庭祯，张翥，王陈超，王明周，涂君平，谢尔玛，张家骅，别应堂. 大鼠热结肠道型气分证模型的构建及肠道结构、IEL和GC数量的变化研究[J]. 畜牧兽医学报, 2014, 45(4): 672-678.
[8]	蔡旋，王静娴，陈小连，郭奇，朱丽慧，徐建雄. 肠道上皮氧化应激细胞模型的研究进展[J]. 畜牧兽医学报, 2014, 45(3): 337-346.
[9]	徐明举;王存连;魏东;利凯;崔红玉;张瑞华;李寸欣;徐彤. H9N2亚型猪流感病毒诱导小鼠肺急性损伤模型的研究[J]. 畜牧兽医学报, 2011, 42(6): 838-844.
[10]	王婷;侯娅丽;刘林;王雅春;张沅. 奶牛杂交群体及其遗传评估的模拟研究[J]. 畜牧兽医学报, 2011, 42(6): 779-784.