β-烟酰胺单核苷酸对牛卵母细胞脂滴含量及冷冻效果的影响

doi:10.11843/j.issn.0366-6964.2023.08.020

摘要/Abstract

摘要： 旨在探明β-烟酰胺单核苷酸（nicotinamide mononucleotide，NMN）对牛卵母细胞脂滴含量和玻璃化冷冻效果的影响。本研究将牛卵母细胞置于含1 μmol·L^-1NMN的培养液中进行体外成熟（in vitro，IVM）、玻璃化和体外受精（in vitro fertilization，IVF），检测了牛卵母细胞的脂滴含量、活性氧（reactive oxygen species，ROS）水平、凋亡水平和发育能力；添加NMN组作为NMN处理组，未添加NMN组作为新鲜对照组；添加NMN且进行玻璃化处理的作为NMN玻璃化组，未添加NMN但进行玻璃化处理的作为玻璃化对照组。结果表明，NMN组牛卵母细胞的脂滴含量、ROS水平、凋亡水平均显著低于新鲜对照组（P<0.05），卵裂率（（89.57±7.58）%）和囊胚率（（45.63±3.78）%）均显著高于新鲜对照组（（80.77±0.70）%，（36.90±1.20）%，P<0.05）；同时，NMN玻璃化组牛卵母细胞的ROS水平和凋亡水平也显著低于玻璃化对照组（P<0.05），并且解冻后的存活率（（97.25±0.11）%）、卵裂率（（75.47±1.11）%）和囊胚率（（33.75±1.43）%）均显著高于玻璃化对照组（（89.29±1.16）%、（52.00±1.26）%、（15.38±1.73）%，P<0.05）。本研究结果表明，添加NMN可以有效降低牛卵母细胞的脂滴含量，降低玻璃化牛卵母细胞的ROS水平和凋亡水平，从而提高玻璃化牛卵母细胞的发育能力。

关键词: NMN, 卵母细胞, 脂滴, 玻璃化, 活性氧, 凋亡

Abstract: The aim of this study was to investigate the effect of β-nicotinamide mononucleotide (NMN) on the lipid droplet content and vitrification freezing of bovine oocytes. In this study, bovine oocytes were placed in medium containing 1 μmol·L^-1NMN for in vitro maturation (IVM), vitrification and in vitro fertilization (IVF). The lipid droplet content, reactive oxygen species (ROS) level, apoptosis level and developmental ability of bovine oocytes were examined. The group with the addition of NMN was used as a NMN group; The group without the addition of NMN was used as a fresh control group; The group with the addition of NMN and vitrification was used as a NMN vitrification group; The group without the addition of NMN but with vitrification was used as a vitrification control group. The lipid droplet content, ROS level, and apoptosis level in bovine oocytes of the NMN group were significantly lower than those in the fresh control group (P<0.05), and the cleavage rate ((89.57±7.58)%) and blastocyst rate ((45.63±3.78)%) were significantly higher than those of the fresh control group ((80.77±0.70)%, (36.90±1.20)%) (P<0.05). Meanwhile, the ROS level and apoptosis level of bovine oocytes in the NMN vitrification group were significantly lower than those in the vitrification control group (P<0.05), and the survival rate ((97.25±0.11)%), cleavage rate ((75.47±1.11)%) and blastocyst rate ((33.75±1.43)%) after thawing were significantly higher than those in the vitrification control group ((89.29±1.16)%, (52.00±1.26)%, (15.38±1.73)%, P<0.05). In conclusion, the addition of NMN could effectively reduce the lipid droplet content of bovine oocytes and decrease the ROS level and apoptosis level of vitrified bovine oocytes, thus improving the developmental ability of vitrified bovine oocytes.

Key words: NMN, oocyte, lipid droplet, vitrification, ROS, apoptosis

中图分类号:

S823.3

徐茜, 杨柏高, 张航, 冯肖艺, 郝海生, 杜卫华, 朱化彬, 张培培, 赵学明. β-烟酰胺单核苷酸对牛卵母细胞脂滴含量及冷冻效果的影响[J]. 畜牧兽医学报, 2023, 54(8): 3348-3357.

XU Xi, YANG Baigao, ZHANG Hang, FENG Xiaoyi, HAO Haisheng, DU Weihua, ZHU Huabin, ZHANG Peipei, ZHAO Xueming. Effects of NMN on Lipid Droplet Content and Cryopreservation Effect of Bovine Oocytes[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3348-3357.

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