家畜早期妊娠诊断的研究进展

doi:10.11843/j.issn.0366-6964.2023.05.002

摘要/Abstract

摘要： 本文从原理、方法、诊断时间和准确率等方面对家畜妊娠临床诊断法、实验室诊断法以及胎儿游离DNA检测法进行综述,总结了不同妊娠诊断方法的优缺点、发展趋势以及实际应用效果,为探索不同应用场景下适宜的家畜妊娠诊断方法提供参考。传统的临床诊断法具有快捷经济的优点,但准确率受人为操作影响较大;实验室诊断法提高了准确率,提早了检测时间,但存在成本高和检测标准不统一的局限性;胎儿游离DNA检测法可用于家畜胎儿性别鉴定,而非性别依赖性胎儿DNA特异性标志物仍需进一步探索。

关键词: 放射免疫分析法, 酶免疫测定法, 早期妊娠因子, 妊娠相关糖蛋白, 胎儿游离DNA

Abstract: This article reviewed the clinical diagnostics, laboratory diagnostics and circulating cell-free fetal DNA(ccffDNA) detection of pregnancy from the following four aspects:principle, method, diagnostic time and accuracy. Through the comparison and comprehensive analysis of the above methods, The advantages, limitations, research trends and the actual application effects of the above diagnostic methods were summarized, in order to provide suitable methods of livestock pregnancy diagnosis applied in different situations. The traditional clinical diagnostics have the double advantages of fast and cheap, but the accuracy is susceptible to the artificial operation. Laboratory diagnostics are earlier and more accurate, but they have disadvantages of lacking unified standard and costing more. The detection of ccffDNA can be used to identify fetal sex in domestic animals, whereas the specific markers of non-sex-dependent fetal DNA are still needed to be explored further.

Key words: radioimmunoassay, enzyme immunoassay, early pregnancy factor, pregnancy-associated glycoproteins, circulating cell-free fetal DNA

中图分类号:

S814.8

李雯乔, 李发弟, 王新基, 乐祥鹏. 家畜早期妊娠诊断的研究进展[J]. 畜牧兽医学报, 2023, 54(5): 1782-1791.

LI Wenqiao, LI Fadi, WANG Xinji, YUE Xiangpeng. Advance of Early Pregnancy Diagnosis in Livestock[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 1782-1791.

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