蒙古马全身主要骨骼肌表型谱的构建及比较研究

doi:10.11843/j.issn.0366-6964.2023.02.017

畜牧兽医学报 ›› 2023, Vol. 54 ›› Issue (2): 596-607.doi: 10.11843/j.issn.0366-6964.2023.02.017

蒙古马全身主要骨骼肌表型谱的构建及比较研究

贾紫洁¹, 图格琴¹, 丁文淇¹, 任秀娟¹, 刘慧莹¹, 李欣泽¹, 翠芳², 芒来^1*, 白东义^1*

1. 内蒙古农业大学动物科学学院内蒙古自治区马遗传育种与繁殖重点实验室农业农村部马属动物种业创新部省共建重点实验室农业农村部马属动物遗传育种繁殖科学观测实验站内蒙古农业大学马属动物研究中心, 呼和浩特 010018;
2. 内蒙古赤峰市阿鲁科尔沁旗动物疫病预防控制中心, 赤峰 025550

收稿日期:2022-07-01 出版日期:2023-02-23 发布日期:2023-02-21
通讯作者: 白东义,主要从事马属动物遗传育种与繁殖研究,E-mail:baidongyi1983@163.com;芒来,主要从事马属动物遗传育种与繁殖研究,E-mail:dmanglai@163.com
作者简介:贾紫洁(1997-),女,内蒙古包头人,硕士生,主要从事动物遗传育种研究,E-mail:925022732@qq.com
基金资助:
国家自然科学基金国际（地区）合作与交流项目（31961143025）；内蒙古自治区自然科学基金面上项目（2019MS03064）；自治区科技重大专项（ZD20190039；2021ZD0018）；内蒙古农业大学动物科学学院标志性成果专项资金项目（BZCG202115）

Construction and Comparative Study of the Phenotypic Spectrum of the Main Skeletal Muscles Throughout the Mongolian Horse

JIA Zijie¹, BAO Tugeqin¹, DING Wenqi¹, REN Xiujuan¹, LIU Huiying¹, LI Xinze¹, CUI Fang², DUGARJAVIIN Manglai^1*, BAI Dongyi^1*

1. Equine Zoology Research Center, Scientific Observation and Experimental Station of Equine Animal Genetics, Breeding and Reproduction of Ministry of Agriculture and Rural Affairs, Key Laboratory of Equus Germplasm Innovation(Co-construction by Ministry and Province) of Ministry of Agriculture and Rural Affairs, Key Laboratory of Equine Genetics, Breeding and Reproduction of Inner Mongolia, College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China;
2. Animal Disease Prevention and Control Center of Arukorqin Banner, Chifeng City, Inner Mongolia, Chifeng 025550, China

Received:2022-07-01 Online:2023-02-23 Published:2023-02-21

摘要/Abstract

摘要： 为探究蒙古马骨骼肌在运动和肉质方面的特点，本研究选取2匹5岁健康未调教的成年公马全身主要骨骼肌52块（2匹×26块）作为研究对象。试验采用石蜡切片、免疫组化、HE染色的方法得到26个部位骨骼肌形态、快慢肌纤维分布情况、肌纤维横截面积。因不同部位骨骼肌存在差异，本研究将肌肉组织分为5组（头颈部、前肢、躯干部、腹壁侧、后肢）分别进行统计。将其中对应的24个部位与前期得到的纯血马骨骼肌快慢肌纤维比例进行分组比对。结果得到蒙古马全身主要骨骼肌的表型谱，其中颈锯肌、冈上肌、臂二头肌、腕桡侧屈肌、腓肠肌、菱形肌、胸腹侧锯肌的慢肌纤维占比较低；腕桡侧伸肌、臂三头肌、背最长肌、臀中肌慢肌纤维占比达70%以上；且慢肌纤维占比与肌纤维横截面积呈负相关。蒙古马骨骼肌慢肌纤维占比基本高于纯血马，前肢、躯干部差异极显著（P<0.01），后肢、腹壁侧差异显著（P<0.05）。其中蒙古马与纯血马在臂三头肌、臀中肌、半膜肌和背最长肌慢肌纤维的差值大于50%。结果表明，蒙古马颈锯肌、冈上肌、臂二头肌、腕桡侧屈肌、腓肠肌、菱形肌、胸腹侧锯肌具有耐力训练的潜力；腕桡侧伸肌、臂三头肌、背最长肌、臀中肌品质好，可为马肉开发的重点；臂三头肌、臀中肌、半膜肌和背最长肌可作为马运动性能检测的重点肌肉。本研究结果可为蒙古马运动训练及肉品质开发提供理论基础。

关键词: 蒙古马, 骨骼肌, 肌纤维类型

Abstract: To explore the characteristics of Mongolian horse skeletal muscle in terms of movement and meat quality, 52 pieces of major skeletal muscle (2×26 pieces) of 2 5-year-old healthy untaught adult stallions were selected as the research objects. Paraffin sections, immunohistochemistry and HE staining were used to obtain skeletal muscle morphology, fast and slow muscle fiber distribution, and average cross-sectional area of muscle fibers of 26 sites. Due to the differences of skeletal muscles at different parts, the muscle tissue was divided into 5 groups (head and neck, forelimbs, trunk, abdominal wall side and hind limbs) for statistics. The corresponding 24 parts were compared with the thoroughpure breds skeletal muscle obtained in the previous studies in the proportion of fast and slow muscle fibers.The results showed that the phenotype spectrum of the main skeletal muscles of Mongolian horses. Among them, the proportion of slow muscle fibers in cervical serratus, supraspinatus, biceps, wrist radial flexor, gastrocnemius, rhomboid muscle and thoracoventrlateral serratus muscle was relatively low. The proportion of slow muscle fibers in wrist radial extensors, triceps brachia, longest dorsal muscle and gluteus mesoamericas accounted for more than 70%; the proportion of slow muscle fibers was negatively correlated with the cross-sectional area of muscle fibers. The proportion of slow muscle fibers in Mongolian horse skeletal muscle was basically higher than that in thoroughpure, the difference in forelimbs and trunks were very significant (P<0.01), and the differences in hindlimbs and abdominal wall side were significant (P<0.05). Among them, the difference of the proportion of slow muscle fibers between Mongolian horses and thoroughbred horses in brachial triceps, longest dorsal muscle, gluteus medius and semimembranous muscle were greater than 50%. The results indicate that serratus equinagia, supraspinatus, biceps brachia, flexor carpal radial, gastrocnemius, rhomboid and thoracoventral serratus muscle have the potential for endurance training. The wrist radial extensors, triceps brachia, longus dorsius and gluteus medius have good quality and can be the focus of horse meat development; and the triceps arm, dorsal maximus, gluteus medius and semimembranous muscles can be used as the key muscles of horse athletic performance testing. These results provides a theoretical basis for Mongolian horse sports training and meat quality development.

Key words: Mongolian horse, skeletal muscle, muscle fiber type

中图分类号:

S821.2

贾紫洁, 图格琴, 丁文淇, 任秀娟, 刘慧莹, 李欣泽, 翠芳, 芒来, 白东义. 蒙古马全身主要骨骼肌表型谱的构建及比较研究[J]. 畜牧兽医学报, 2023, 54(2): 596-607.

JIA Zijie, BAO Tugeqin, DING Wenqi, REN Xiujuan, LIU Huiying, LI Xinze, CUI Fang, DUGARJAVIIN Manglai, BAI Dongyi. Construction and Comparative Study of the Phenotypic Spectrum of the Main Skeletal Muscles Throughout the Mongolian Horse[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(2): 596-607.

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蒙古马全身主要骨骼肌表型谱的构建及比较研究

Construction and Comparative Study of the Phenotypic Spectrum of the Main Skeletal Muscles Throughout the Mongolian Horse

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