云南独龙鸡和白来航鸡卵巢组织转录组水平的比较分析

doi:10.11843/j.issn.0366-6964.2022.01.005

摘要/Abstract

摘要： 旨在利用RNA-seq技术筛选和分析云南独龙鸡和白来航鸡卵巢组织的差异表达基因，为独龙鸡后续基因功能、品种改良和种质特性等研究奠定基础。本研究采集了3只43周龄健康状况良好的独龙鸡卵巢组织进行转录组测序，并与相似饲养条件和周龄的白来航鸡卵巢组织进行比较。结果表明，与白来航鸡相比，独龙鸡中共有1 273个差异表达基因，其中上调基因522个，下调基因751个。GO和KEGG富集分析显示，差异表达基因主要参与蛋白水解、翻译、细胞外泌体、膜组分、结合和催化等过程，在蛋白合成、ECM-受体相互作用、氧化磷酸化、粘着斑等通路中起到重要作用。对显著性通路分析发现独龙鸡在蛋品质和免疫抗性上具有一定优势，但在产蛋性能上与白来航鸡还存在一定差距。本研究筛选到的RPL22、ATP5I、COX5A、JAKMIP1、CATH2基因主要富集在抗性、产蛋性能和蛋品质相关通路中，可作为重要候选基因进一步深入研究。

关键词: 独龙鸡, 白来航鸡, 卵巢组织, 转录组测序, 差异表达基因

Abstract: The aim of this study was to screen and analyze differentially expressed genes in ovarian tissues of Yunnan Dulong chicken and White Leghorn chicken using RNA-seq technology, and to lay the foundation for subsequent studies on gene function, breed improvement, and germplasm characteristics of Dulong chicken. In this study, the ovarian tissues of three 43-week-old Dulong chickens in good health were collected for transcriptome sequencing and compared with the ovarian tissue of White Leghorn Chicken in similar rearing conditions and age. The results showed that there were 1 273 differentially expressed genes in Dulong chickens compared with White Leghorn chicken, among which 522 genes were up-regulated and 751 genes were down-regulated. GO and KEGG enrichment analysis showed that differentially expressed genes were mainly involved in proteolysis, translation, exosomes, membrane components, binding, and catalysis, and played an important role in protein synthesis, ECM-receptor interaction, oxidative phosphorylation, adhesion plaque, and other pathways. Significant enrichment pathway analysis showed that Dulong chickens had certain advantages in egg quality and immune resistance, but there was still a certain gap between Dulong chickens and White Leghorn chickens in laying performance. RPL22, ATP5I, COX5A, JAKMIP1, CATH2 screened in this study were mainly enriched in the pathways related to resistance, egg-laying performance, and egg quality, which could be used as important candidate genes for further study.

Key words: Dulong chicken, White Leghorn chicken, ovarian tissue, RNA-seq, differentially expressed gene

中图分类号:

S831.2

任宇, 丁玥竹, 农燕鸿, 郭爱伟, 刘莉莉. 云南独龙鸡和白来航鸡卵巢组织转录组水平的比较分析[J]. 畜牧兽医学报, 2022, 53(1): 43-52.

REN Yu, DING Yuezhu, NONG Yanhong, GUO Aiwei, LIU Lili. Comparative Analysis of Transcriptome Levels in Ovarian Tissues of Yunnan Dulong Chicken and White Leghorn Chicken[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(1): 43-52.

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