豪猪血蜱卵蛋白成分初步鉴定及其功能分类

doi:10.11843/j.issn.0366-6964.2021.010.020

摘要/Abstract

摘要： 旨在探明豪猪血蜱卵原生质蛋白成分，为探索干扰蜱类胚胎发育和新型控蜱策略奠定基础。采用FASP法消化豪猪血蜱卵蛋白提取物，LC/MS/MS法对其进行分析、检测，搜索褐黄血蜱卵巢转录组文库、唾液腺转录组文库和中肠转录组文库，分别检出特异性肽段359、312、357条，鉴定多肽108、170、161条，终而鉴定高可信蛋白103种。豪猪血蜱蜱卵原生质中富含酶类、蛋白酶抑制剂、转运蛋白、细胞骨架蛋白、蛋白质合成与修饰、分泌蛋白以及未鉴定的蛋白共8类蛋白。

关键词: 卵, 液相色谱-串联质谱, 蛋白酶, 转运蛋白, 蛋白酶抑制剂

Abstract: The identification of protein components of Haemaphysalis hystricis egg protoplasm illustrated the tick embryo protein profile and laid a foundation for the discovery of new anti-tick strategies. The egg protein extracts of Haemaphysalis hystricis was digested with trypsin according the FASP procedure and the LC/MS/MS method was employed to detect and identify peptides. The MS data were searched against the ovarian, salivary gland and midgut transcriptomic libraries of Haemaphysalis flava, and the 359, 312 and 357 specific peptides were detected, then the 108, 170 and 161 polypeptides were identified. Eventrually, the 103 proteins of them were considered as high confidence proteins (peptides ≥ 2). These high confidence proteins were involved in eight groups including enzymes, protease inhibitors, transporters, cytoskeleton proteins, protein synthesis modifications, secreted proteins and uncharacterized proteins.

Key words: egg, LC-MS/MS, protease, transporter, protease inhibitor

中图分类号:

S852.746

刘金宝, 程天印. 豪猪血蜱卵蛋白成分初步鉴定及其功能分类[J]. 畜牧兽医学报, 2021, 52(10): 2895-2904.

LIU Jinbao, CHENG Tianyin. Preliminary Identification and Functional Classification of Haemaphysalis hystricis Egg Protein Components[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(10): 2895-2904.

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