CRISPR/Cas系统及其在结核分枝杆菌研究中的应用

doi:10.11843/j.issn.0366-6964.2020.11.001

畜牧兽医学报 ›› 2020, Vol. 51 ›› Issue (11): 2613-2621.doi: 10.11843/j.issn.0366-6964.2020.11.001

CRISPR/Cas系统及其在结核分枝杆菌研究中的应用

宋禹昊¹, 李东¹, 孙江洋², 时坤^2,4, 李健明^2,4, 宗颖^2,4, 赵丹³, 曾范利^2,4*, 杜锐^2,4,5,6*

1. 吉林农业大学动物科学技术学院, 长春 130118;
2. 吉林农业大学中药材学院, 长春 130118;
3. 吉林农业大学农业质量标准与检测技术研究中心, 长春 130118;
4. 梅花鹿药用资源利用关键技术研究室, 长春 130118;
5. 动物生产及产品质量安全教育部重点实验室, 长春 130118;
6. 吉林省梅花鹿高效养殖和产品开发技术工程研究中心, 长春 130118

收稿日期:2020-05-18 出版日期:2020-11-25 发布日期:2020-11-20
通讯作者: 曾范利,主要从事动物疫病学及免疫学研究,E-mail:zengfanli@jlau.edu.cn;杜锐,主要从事动物疫病学及免疫学研究,E-mail:durui@jlau.edu.cn
作者简介:宋禹昊(1995-),吉林梨树人,硕士生,主要从事经济动物疫病与病原学研究,E-mail:haoqixinsong@163.com
基金资助:
科技部国家重点研发计划项目（2018YFD0500900；2018YFC1706601）；吉林省科技厅科技支撑计划（20180201010YY）

CRISPR/Cas System and Its Application in the Study of Mycobacterium tuberculosis

SONG Yuhao¹, LI Dong¹, SUN Jiangyang², SHI Kun^2,4, LI Jianming^2,4, ZONG Ying^2,4, ZHAO Dan³, ZENG Fanli^2,4*, DU Rui^2,4,5,6*

1. College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China;
2. College of Chinese Medicine Materials, Jilin Agricultural University, Changchun 130118, China;
3. Research Center of Agriculture Quality Standard and Detecting Technique, Jilin Agricultural University, Changchun 130118, China;
4. Research Laboratory on Key Technology of Development and Resource Utilization Sika Deer, Changchun 130118, China;
5. Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Changchun 130118, China;
6. Jilin Province Sika Deer Efficient Breeding and Product Development Technology Engineering Research Center, Changchun 130118, China

Received:2020-05-18 Online:2020-11-25 Published:2020-11-20

摘要/Abstract

摘要： 簇状规则间隔的短回文重复序列（CRISPR）/CRISPR相关蛋白（Cas）系统是一种针对入侵核酸的可遗传性原核适应性免疫系统。CRISPR/Cas系统可以产生高度特异性的基因双链断裂，并通过非同源末端连接（NHEJ）或者同源重组（HR）进行修复，如今已经成为许多生物方便有效的基因组编辑工具。结核分枝杆菌因其基因操作的复杂性，其基因组研究存在诸多困难，因此，CRISPR/Cas系统的出现对结核分枝杆菌的研究具有里程碑式意义。本文围绕CRISPR/Cas系统在结核分枝杆菌中的应用，综述了国内外最新的研究进展，为结核分枝杆菌研究方法的选择提供参考。

关键词: 结核分枝杆菌, CRISPR/Cas系统, CRISPR/dCas9, CRISPR-Cas12a

Abstract: Clustered regularly interspaced short palindromic repeats (CRISPR) / CRISPR-related protein (Cas) system is a heritable prokaryotic adaptive immune system against invading nucleic acids. It is an editing tool for many organisms that can generate highly specific gene double-strand breaks and repair them by non-homologous end joining (NHEJ) or homologous recombination (HR). Due to the complexity of its genetic manipulation, Mycobacterium tuberculosis genome research has many difficulties. Therefore, the emergence of the CRISPR/Cas system has a milestone significance for the research of Mycobacterium tuberculosis. In this review, we focus on the application of the CRISPR/Cas system in Mycobacterium tuberculosis and summarizes the present research progress in order to provide a reference for the selection of Mycobacterium tuberculosis research methods.

Key words: Mycobacterium tuberculosis, CRISPR/Cas system, CRISPR/dCas9, CRISPR-Cas12a

中图分类号:

宋禹昊, 李东, 孙江洋, 时坤, 李健明, 宗颖, 赵丹, 曾范利, 杜锐. CRISPR/Cas系统及其在结核分枝杆菌研究中的应用[J]. 畜牧兽医学报, 2020, 51(11): 2613-2621.

SONG Yuhao, LI Dong, SUN Jiangyang, SHI Kun, LI Jianming, ZONG Ying, ZHAO Dan, ZENG Fanli, DU Rui. CRISPR/Cas System and Its Application in the Study of Mycobacterium tuberculosis[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(11): 2613-2621.

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CRISPR/Cas系统及其在结核分枝杆菌研究中的应用

CRISPR/Cas System and Its Application in the Study of Mycobacterium tuberculosis

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