绵羊肌肉组织MyoG和 PID1 基因的表达及其与肌内脂肪含量的关系

doi:doi: 10.11843/j.issn.0366-6964.2016.10.006

摘要/Abstract

摘要：

本研究旨在探究绵羊不同部位肌肉MyoG和PID1基因mRNA的发育变化规律，分析MyoG和PID1 基因mRNA表达水平对肌内脂肪沉积的影响。选取2、4、5、6 月龄敖汉细毛羊公羔和12月龄敖汉细毛羊成年公羊各5只，屠宰后取背最长肌和股二头肌检测肌内脂肪（Intramuscular fat，IMF）含量，用实时荧光定量 PCR 检测两个部位肌肉MyoG和PID1 mRNA 表达量，并进一步分析表达量与肌内脂肪含量的关系。结果表明，2~5月龄时，背最长肌和股二头肌的IMF含量均随着月龄的增加而增加；而5~12月龄时则基本保持不变；同月龄背最长肌IMF 含量极显著高于股二头肌（P＜0.01）。两个部位肌肉MyoG和PID1 mRNA 表达的发育模式有所不同，具有组织特异性。背最长肌MyoG基因表达量5月龄最高（P＜0.01），PID1基因表达量6月龄最高（P＜0.05），均呈上升-下降趋势；股二头肌MyoG和 PID1基因各月龄之间表达量均差异极显著（P＜0.01），MyoG 基因表达量随着月龄增长呈上升-下降趋势，PID1基因表达量大体呈下降-上升趋势。同月龄MyoG和PID1 表达量存在组织差异。相关分析表明，MyoG 和PID1 表达量与 IMF 含量呈不同程度正相关。综上，MyoG基因表达对绵羊肌内脂肪的沉积可能有正调控作用，而PID1基因表达可能对肌内脂肪的沉积产生一定的影响。

Abstract:

The objectives of this study were to investigate the developmental changes of Myogenin (MyoG) and Phosphotyrosine interaction domain containing 1 (PID1) mRNA expression in different parts of muscles in sheep and research its effect on intramuscular fat (IMF) accumulation.Five Aohan fine wool sheep were slaughtered at 2，4，5，6，12 months，respectively，to collect samples from longissimus dorsi and biceps femoris，for determining the IMF content.The mRNA expression of the MyoG and PID1 in 2 parts of muscles was investigated by real-time PCR，and association between their mRNA levels and IMF contents were also analyzed.The results showed that，2-5 month，with growing of the age，the IMF contents of longissimus dorsi and biceps femoris increased.And 5-12 month，the IMF contents remained unchanged.The IMF content of longissimus dorsi were extremely significantly higher than biceps femoris (P<0.01) at the same age.MyoG and PID1 mRNA expression level was different in 2 parts of muscles and had tissues-dependent.In longissimus dorsi， MyoG expression level was the highest (P<0.01) in 5 month old sheep，PID1 expression level was the highest (P<0.05) in 6 month old sheep，there was an increase and then decrease trend in the 2 gene expression.In biceps femoris，the MyoG and PID1 expression level difference was extremely significant(P<0.01) among different month ages，and MyoG expression level presented increased and declined slowly with growing，PID1 expression level presented declined-increased with growing.In the same month，MyoG and PID1 expression level had significant difference in different parts.Correlation analysis showed that MyoG and PID1 expression level was inordinately positively related to IMF content.In conclusion，MyoG expression has positively effect on IMF accumulation in sheep，and PID1 expression is likely to influence on IMF accumulation.

中图分类号:

S826
S813.3

栾兆进，贺建宁，程明，刘开东，曲绪仙，柳楠. 绵羊肌肉组织MyoG和 PID1 基因的表达及其与肌内脂肪含量的关系[J]. 畜牧兽医学报, 2016, 47(10): 1986-1994.

LUAN Zhao-jin，HE Jian-ning，CHENG Ming，LIU Kai-dong，QU Xu-xian，LIU Nan. Association of the MyoG and PID1 Gene Expression with Intramuscular Fat Content in Sheep Muscles [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(10): 1986-1994.

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: https://www.xmsyxb.com/CN/doi: 10.11843/j.issn.0366-6964.2016.10.006

https://www.xmsyxb.com/CN/Y2016/V47/I10/1986

参考文献

［1］赵有璋.羊生产学［M］.北京：中国农业出版社，2002：69-75.
ZHAO Y Z.Production science of sheep and goat［M］.Beijing：China Agriculture Press，2002：69-75.(in Chinese)
［2］URBAN T，MIKOLASOVA R，KUCIEL J，et al.A study of associations of the H-FABP genotypes with fat and meat production of pigs［J］. J Appl Genet，2002，43(4)：505-510.
［3］杜琛，李金泉，陈秀娟.白绒山羊 PPARγ 基因RNA干扰慢病毒载体的构建及对肌内脂肪细胞增殖分化的影响［J］.畜牧兽医学报，2016，47(4)：671-678.
DU C，LI J Q，CHEN X J.Construction of lentiviral RNAi vector of PPARγ gene in cashmere goat and its effect on proliferation and differentiation of intramuscular adipocytes［J］. Acta Veterinaria et Zootechnica Sinica，2016，47(4)：671-678.(in Chinese)
［4］WANG Y H，BYRNE K A，REVERTER A，et al.Transcriptional profiling of skeletal muscle tissue from two breeds of cattle［J］.Mamm Genome，2005，16(3)：201-210.
［5］郝称莉，李齐发，乔永，等.湖羊肌肉组织H-FABP和PPARγ基因表达水平与肌内脂肪含量的相关研究［J］.中国农业科学，2008，41(11)：3776-3783.
HAO C L，LI Q F，QIAO Y，et al.Association of the H-FABP and PPARγ gene expression with intramuscular fat content in Hu sheep muscles［J］.Scientia Agricultura Sinica，2008，41(11)：3776-3783.(in Chinese)
［6］MEUWISSEN T H E，GODDARD M E.The use of marker haplotypes in animal breeding schemes［J］.Genet Sel Evol，1996，28(2)：161-176.
［7］HASTY P，BRADLEY A，MORRIS J H，et al.Muscle deficiency and neonatal death in mice with a targeted mutation in the myogenin gene［J］.Nature，1993，364 (6437)：501-506.
［8］苏艳红，齐鸷，周越，等.Caveolin-3，MyoGenin，MyoD在大鼠力竭运动后骨骼肌损伤过程的时程表达［J］.北京体育大学学报，2012，35(12)：57-61.
SU Y H，QI Z，ZHOU Y，et al.Effect of Caveolin-3，Myogenin and MyoD on the rehabilitation of skeletal muscle injury after rats’exhausting exercise［J］.Journal of Beijing Sport University，2012，35(12)：57-61.(in Chinese)
［9］BERTI F，NOGUEIRA J M，WOHRLE S，et al.Time course and side-by-side analysis of mesodermal，pre-myogenic，myogenic and differentiated cell markers in the chicken model for skeletal muscle formation［J］.J Anat，2015，227(3)：361-382.
［10］杜琛.绒山羊肌内脂肪沉积特征及脂肪细胞分化机理的研究［D］.呼和浩特：内蒙古农业大学，2014.
DU C.Characteristics of intramuscular fat deposition and mechanism of adipocyte differentiation in cashmere goat［D］.Hohhot：Inner Mongolia Agricultural University，2014.(in Chinese)
［11］BOCIAN M，CIESLAK D，GRAJEWSKA S，et al.A relationship between genotypes at MYOG，MYF3 and MYF5 loci and carcass meat and fat deposition traits in pigs［J］.Anim Sci Pap Rep，2002，20(2)：79-92.
［12］ZHU L，LI X W.The genetic effects of MyoG gene［J］.Hereditas (Beijing)，2005，27(5)：710-714.
［13］姜美华，巨婷婷，刘洋，等.成肌细胞成脂过程中PPARγ、C/EBPα和Myogenin基因启动子的甲基化变化［J］.中国农业科学，2013，46(14)：3010-3021.
JIANG M H，JU T T，LIU Y，et al.Dynamics of PPARγ，C/EBPα and Myogenin gene promoter methylation during myoblasts intracellular lipid accumulation［J］.Scientia Agricultura Sinica，2013，46(14)：3010-3021.(in Chinese)
［14］SHI C M，XU G F，YANG L，et al.Overexpression of TFAM protects 3T3-L1 adipocytes from NYGGF4 (PID1) overexpression-induced insulin resistance and mitochondrial dysfunction［J］.Cell Biochem Biophys，2013，66(3)：489-497.
［15］ZHAO Y，ZHANG C，CHEN X，et al.Overexpression of NYGGF4（PID1） induces mitochondrial impairment in 3T3-L1 adipocytes［J］.Mol Cell Biochem，2010，340(1-2)：41-48.
［16］李娟，史春梅，季晨博，等.NYGGF4(PID1)基因过表达对脂肪细胞中脂肪酸合成酶表达的影响［J］.江苏医药，2012，38(19)：2233-2235.
LI J，SHI C M，JI C B，et al.Effect of NYGGF4(PID1) gene over-expression on fatty acid synthase in adipocytes［J］.Jiangsu Medical Journal，2012，38(19)：2233-2235.(in Chinese)
［17］XU H，XU G，WANG D，et al.Molecular cloning and tissue distribution of the phosphotyrosine interaction domain containing 1 (PID1) gene in Tianfu goat［J］.Gene，2013，515(1)：71-77.
［18］殷捷，凌英会，丁建平，等.山羊PID1基因组织表达谱及其与肌内脂肪含量的相关性［J］.江苏农业学报，2014，30(4)：784-789.
YIN J，LING Y H，DING J P，et al.Expression of PID1 and the association with intramuscular fat in goat［J］.Jiangsu Journal of Agricultural Sciences，2014，30(4)：784-789.(in Chinese)
［19］钱源，曾勇庆，杜金芳，等.猪PID1基因CDS区的克隆及其mRNA表达与肌内脂肪沉积关系［J］.遗传，2010，32(11)：68-73.
QIAN Y，ZENG Y Q，DU J F，et al.CDS cloning and relationship between intramuscular fat content and mRNA expression of PID1 gene in pig［J］.Hereditas，2010，32(11)：68-73.(in Chinese)
［20］张晓卉.民猪MyoG基因的克隆、表达与启动子核心区的定位［D］.哈尔滨：东北农业大学，2012.
ZHANG X H.The cloning，expression and the core area promoter positioning of min pig MyoG gene［D］.Harbin：Northeast Agricultural University，2012.(in Chinese)
［21］CHIBA T，YAMAZA H，KOMATSU T，et al.Pituitary growth hormone suppression reduces resistin expression and enhances insulin effectiveness：relationship with calonic restriction［J］.Exp Gerontol，2008，43(6)：595-600.
［22］钱源，曾勇庆，崔景香，等.莱芜猪PID1基因的功能分析及表达谱研究［J］.畜牧兽医学报，2011，42(5)：621-628.
QIAN Y，ZENG Y Q，CUI J X，et al.Functional analysis and tissue specific expression profile of PID1 gene in laiwu pig［J］.Acta Veterinaria et Zootechnica Sinica，2011，42(5)：621-628.(in Chinese)
［23］WU W，GARI W，TONG M，et al.Over-expression of NYGGF4 (PID1) inhibits glucose transport in skeletal myotubes by blocking the IRS1/PI3K/AKT insulin pathway［J］.Mol Genet Metab，2011，102(3)：374-377.
［24］WANG B，ZHANG M，NI Y，et al.Identification and characterization of NYGGF4，a novel gene containing a phosphotyrosine-binding (PTB) domain that stimulates 3T3-L1 preadipocytes proliferation［J］.Gene，2006，379(1)：132-140.
［25］WU D D，IRWIN D M，ZHANG Y P.Molecular evolution of the keratin associated protein gene family in mammals，role in the evolution of mammalian hair［J］.BMC Evol Biol，2008，8：241.
［26］DA COSTA A S，PIRES V M，FONTES C M，et al.Expression of genes controlling fat deposition in two genetically diverse beef cattle breeds fed high or low silage diets［J］.BMC Vet Res，2013，9：118.
［27］GRAUGNARD D E，PIANTONI P，BIONAZ M，et al.Adipogenic and energy metabolism gene networks in longissimus lumborum during rapid post-weaning growth in Angus and Angus×Simmental cattle fed high-starch or low-starch diets［J］.BMC Genomics，2009，10：142.

[1]	王海波, 占今舜, 谷志勇, 陈新锋, 潘月, 贾浩滨, 钟小军, 李开嵘, 赵生国, 霍俊宏. 湖羊与三元杂交绵羊夏杜湖、夏澳湖肉质特性比较研究[J]. 畜牧兽医学报, 2024, 55(1): 110-119.
[2]	段香茹, 康佳, 杨若晨, 单新雨, 李太春, 赵雯, 张英杰, 刘月琴. L-半胱氨酸对绵羊卵巢颗粒细胞增殖、凋亡和类固醇激素分泌的影响[J]. 畜牧兽医学报, 2024, 55(1): 179-191.
[3]	宋鹏琰, 王思伟, 岳巧娴, 张寅梁, 陈晓勇, 周荣艳. 绵羊miR-200b启动子鉴定及其对卵泡颗粒细胞线粒体功能的影响[J]. 畜牧兽医学报, 2023, 54(12): 5066-5076.
[4]	韩浩园, 李世凯, 杨瑞巧, 李曼曼, 李君, 哈斯, 赵金艳, 魏红芳, 权凯. 基于转录组测序挖掘槐山羊高繁关键候选基因[J]. 畜牧兽医学报, 2023, 54(12): 5077-5090.
[5]	陈聪慧, 段春辉, 杨欣雨, 夏翠, 郭云霞, 纪守坤, 严慧, 刘月琴, 张英杰. 运输应激不同干预方式对羔羊宰后肌肉能量代谢的影响[J]. 畜牧兽医学报, 2023, 54(12): 5101-5111.
[6]	栗登攀, 马克岩, 韩金涛, 白雅琴, 李讨讨, 马友记. 永登七山羊全基因组选择信号检测分析[J]. 畜牧兽医学报, 2023, 54(11): 4577-4588.
[7]	赵雪洋, 李丹妮, 王钰晨, 郭磊, 王丽, 黄杰, 焦仪强, 安小鹏, 张希云, 张磊, 宋宇轩. 东湖杂交羊产羔性状的GWAS分析及候选基因GRID2的验证[J]. 畜牧兽医学报, 2023, 54(11): 4625-4635.
[8]	张寒月, 赵丹, 梁煜, 赵弼时, 范蒙丹, 乔利英, 刘建华, 杨凯捷, 潘洋洋, 刘文忠. miR-150靶向AOC3调控绵羊前体脂肪细胞分化的研究[J]. 畜牧兽医学报, 2023, 54(8): 3262-3274.
[9]	刘林丽, 彭雪兰, 李博, 程乐凡, 次仁拉姆, 张恩平. 过表达UCP3基因对萨湖羊前体脂肪细胞分化的影响[J]. 畜牧兽医学报, 2023, 54(8): 3275-3285.
[10]	贺名扬, 马钰静, 王泳, 杨若晨, 刘月琴, 张英杰, 段春辉. 褪黑激素对绵羊卵巢颗粒细胞增殖、凋亡、类固醇激素分泌的影响[J]. 畜牧兽医学报, 2023, 54(8): 3313-3324.
[11]	宋美君, 郝科兴, 海思妤, 陈岩, 王静, 胡广东. SRIF-14对绵羊子宫内膜上皮细胞增殖和凋亡的影响[J]. 畜牧兽医学报, 2023, 54(8): 3325-3334.
[12]	李悦欣, 刘爱菊, 马晓菲, 郑忠, 胡伯欣, 智云霞, 田树军. TGFβR1介导TGF-β/Smad信号通路对绵羊颗粒细胞功能的影响[J]. 畜牧兽医学报, 2023, 54(8): 3335-3347.
[13]	马友记, 陈鹏飞, 马青, 吴怡. 不同运动量对滩羊瘤胃菌群多样性的影响[J]. 畜牧兽医学报, 2023, 54(8): 3393-3405.
[14]	王万年, 陈思佳, 郜金荣, 温中豪, 袁梦娇, 张洪志, 庞志旭, 乔利英, 刘文忠. 基于多层感知机的绵羊限性性状基因组选择模拟研究[J]. 畜牧兽医学报, 2023, 54(7): 2824-2835.
[15]	熊程坤, 张道亮, 杨悦, 丁红研, 赵杰, 李玉, 王希春, 冯士彬, 赵畅, 汤继顺, 吴金节. 芦丁对围产期湖羊瘤胃发酵、瘤胃菌群结构及抗氧化性能的影响[J]. 畜牧兽医学报, 2023, 54(7): 2898-2909.