双氢青蒿素对脂多糖诱导的断奶仔猪肝氧化应激的影响

doi:10.11843/j.issn.0366-6964.2019.10.021

畜牧兽医学报 ›› 2019, Vol. 50 ›› Issue (10): 2139-2146.doi: 10.11843/j.issn.0366-6964.2019.10.021

双氢青蒿素对脂多糖诱导的断奶仔猪肝氧化应激的影响

赵永伟, 牛玉, 何进田, 季书立, 张莉莉, 王超, 王恬*

南京农业大学动物科技学院, 南京 210095

收稿日期:2019-04-29 出版日期:2019-10-23 发布日期:2019-10-23
通讯作者: 王恬,主要从事动物营养与调控研究,E-mail:tianwangnjau@163.com
作者简介:赵永伟(1993-),男,江苏泰州人,硕士生,主要从事动物营养与调控研究,Tel:025-84396483,E-mail:450344441@qq.com
基金资助:
国家重点研发计划（2018YFD0501101）

Effects of Dihydroartemisinin on Oxidative Stress in Liver of Weaned Piglets Induced by Lipopolysaccharide

ZHAO Yongwei, NIU Yu, HE Jintian, JI Shuli, ZHANG Lili, WANG Chao, WANG Tian*

College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China

Received:2019-04-29 Online:2019-10-23 Published:2019-10-23

摘要/Abstract

摘要： 为了研究双氢青蒿素（DHA）对断奶仔猪氧化应激的影响，通过脂多糖（LPS）诱导的方法建立了氧化应激模型。选用健康的断奶仔猪30头，随机分成对照组（CON）、模型组（LPS）和处理组（LD，LPS+DHA），每组10个重复。CON组和LPS组饲喂基础日粮，LD组饲喂基础日粮+80 mg·kg^-1 DHA。各组饲喂相应日粮21 d。在宰前4 h对LPS组和LD组按照体重腹腔注射100 μg·kg^-1 LPS，CON组注射相同剂量的无菌生理盐水。结果显示，与CON组相比，LPS组肝中丙二醛（MDA）、蛋白质羰基（PC）、8羟基脱氧鸟苷（8-OHdG）和过氧化氢（H₂O₂）含量显著升高（P<0.05）。同时，LPS组肝中总抗氧化能力（T-AOC）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GPx）活力和还原型谷胱甘肽（GSH）含量显著降低（P<0.05）。与LPS组相比，LD组中MDA、PC、8-OHdG和H₂O₂含量显著降低（P<0.05），CAT、T-AOC和GPx的活力显著提高（P<0.05）。另外，与CON组相比，LPS组肝中核因子E2相关因子2（Nrf2）和CAT的mRNA表达量以及Nrf2和血红素加氧酶1（HO-1）的蛋白表达量均显著降低（P<0.05）。DHA处理可显著（P<0.05）逆转LPS引起的上述变化。结果表明，DHA可能通过Nrf2/ARE信号通路的调节来缓解由LPS引起的断奶仔猪氧化应激，本研究可为畜牧生产中减少氧化应激提供一定的理论依据。

Abstract: In order to study the effects of dihydroartemisinin (DHA) on oxidative stress in weaned piglets, a model of oxidative stress was established by injection of lipopolysaccharide (LPS). Thirty healthy weaned piglets were randomly divided into control group (CON), model group (LPS) and treatment group (LD, LPS + DHA). Each group had 10 replicates. CON group and LPS group were fed with basal diet, LD group was fed with basal diet + 80 mg·kg^-1 DHA. Each group was fed with corresponding diet for 21 days. LPS group and LD group were intraperitoneally injected with 100 μg·kg^-1 LPS at 4 hours before slaughter. CON group was injected with the same dose of sterile saline. Compared with the CON group, the contents of malondialdehyde (MDA), protein carbonyl (PC), 8-hydroxy-2-deoxyguanosine (8-OHdG) and hydrogen peroxide (H₂O₂) in the liver of LPS group were significantly increased (P<0.05). Meanwhile, the activities of total antioxidant capacity (T-AOC), catalase (CAT), glutathione peroxidase (GPx) and the contents of reduced glutathione (GSH) in the liver of LPS group were significantly decreased (P<0.05). Compared with LPS group, the contents of MDA, PC, 8-OHdG and H₂O₂ in LD group decreased significantly (P<0.05), and the activities of CAT, T-AOC and GPx increased significantly (P<0.05). In addition, the expression of nuclear factor E2-related factor 2 (Nrf2) and CAT and the protein expression of Nrf2 and HO-1 in liver of LPS piglets were significantly lower than those of CON group (P<0.05). DHA treatment significantly reversed the above changes induced by LPS (P<0.05). The results showed that DHA could alleviate oxidative stress in weaned piglets induced by LPS by regulating Nrf2/ARE signaling pathway, which provided a theoretical basis for reducing oxidative stress in livestock production.

中图分类号:

赵永伟, 牛玉, 何进田, 季书立, 张莉莉, 王超, 王恬. 双氢青蒿素对脂多糖诱导的断奶仔猪肝氧化应激的影响[J]. 畜牧兽医学报, 2019, 50(10): 2139-2146.

ZHAO Yongwei, NIU Yu, HE Jintian, JI Shuli, ZHANG Lili, WANG Chao, WANG Tian. Effects of Dihydroartemisinin on Oxidative Stress in Liver of Weaned Piglets Induced by Lipopolysaccharide[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(10): 2139-2146.

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双氢青蒿素对脂多糖诱导的断奶仔猪肝氧化应激的影响

Effects of Dihydroartemisinin on Oxidative Stress in Liver of Weaned Piglets Induced by Lipopolysaccharide

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