郁金散对大肠湿热证大鼠回肠、结肠黏膜SIgA和炎性因子的影响

doi:10.11843/j.issn.0366-6964.2019.10.022

摘要/Abstract

摘要： 旨在探讨郁金散对大肠湿热证大鼠回肠、结肠肠黏膜SIgA和炎性因子的影响。体重180~220 g Wistar大鼠40只，随机分为正常对照组、大肠湿热证模型组、自愈组和郁金散治疗组，每组10只。通过饮食设置（高糖高脂饮食）、饥饱失常和灌服白酒并结合高温高湿环境，最后腹腔注射生物因子大肠杆菌，以模拟大肠湿热证发病条件，建立大肠湿热证大鼠模型。郁金散治疗组用郁金散治疗5 d，然后采集回肠和结肠组织制作病理切片，进行组织病理学观察；用ELISA法检测回肠和结肠黏液中免疫球蛋白SIgA含量及组织炎性细胞因子TNF-α、IL-1β、IL-6、IL-10、IL-17和IL-23含量。结果显示，模型大鼠回肠和结肠黏膜上皮细胞变性、坏死、脱落，上皮完整性被破坏，有炎性细胞浸润现象；郁金散治疗组黏膜上皮再生，完整性得到恢复，连续性良好，排列有序，炎性细胞浸润明显减少。模型组大鼠回肠和结肠黏液中的SIgA含量均显著高于正常对照组（P<0.05）；郁金散治疗组显著低于自愈组（P<0.05）。模型组回肠和结肠组织中促炎因子TNF-α、IL-1β、IL-6、IL-17和IL-23含量显著或极显著高于正常对照组（P<0.05或P<0.01），抑炎因子IL-10含量显著或极显著低于正常对照组（P<0.05或P<0.01）；郁金散治疗组回肠组织的上述炎性因子含量与自愈组相比均差异显著（P<0.05）；结肠中的TNF-α、IL-10、IL-23与自愈组差异显著（P<0.05）；IL-1β、IL-6、IL-17与自愈组差异极显著（P<0.01）。结果表明，大肠湿热证模型大鼠回肠和结肠黏膜免疫稳态被打破，表现为SIgA分泌亢进，炎性细胞因子分泌紊乱；郁金散治疗可以促使其显著回调。

Abstract: This study was conducted to explore the effects of Yujin powder (YJP) on SIgA and inflammatory cytokines in ileal and colonic mucosa of Large Intestine Dampness-heat Syndrome (LIDHS) rat. Forty Wistar rats were randomly divided into 4 groups:normal control, LIDHS model, self-healing and YJP treatment group, with 10 rats in each group. The rat model of LIDHS was established through setting such complex factors as high-sugar and high-fat diet, improper diet, high temperature and high humidity environment, drinking and intraperitoneal injection of Escherichia coil to imitate the inducing conditions of LIDHS. After the model being successfully established, the rats of YJP treatment group were treated with YJP for 5 days. And then we collected ileum and colon, made histopathological slices and observed the pathological changes of ileum and colon. Mucous SIgA and tissue TNF-α, IL-1β, IL-6, IL-10, IL-17 and IL-23 in ileum and colon were detected by ELISA method. The results showed that in model rats, the ileal and colonic mucosal epithelium cells showed degeneration, necrosis and exfoliation, the integrality of epithelium was destroyed; and there were inflammatory cells infiltration. In YJP group, mucosal epithelium regenerated, the integrality was recovered, the continuity was good, epithelium cells have a regular arrangement, and inflammatory cells infiltration obviously decreased. The contents of mucous SIgA in ileum and colon in LIDHS model group were significantly higher than those in normal group (P<0.05); those in YJP treatment group were both significantly lower than those in self-healing group (P<0.05). The contents of pro-inflammatory cytokines TNF-α, IL-1β, IL-6, IL-17 and IL-23 in ileum and colon tissue were significantly or extremely significantly higher than those in normal group (P<0.05 or P<0.01). The contents of anti-inflammatory cytokine IL-10 were significantly or extremely significantly lower than those in normal group (P<0.05 or P<0.01). The contents of above inflammatory cytokines in ileum tissue in YJP treatment group were significantly different from those in self-healing group (P<0.05); the contents of TNF-α, IL-10 and IL-23 in colon were significantly different from those in self-healing group (P<0.05); the contents of IL-1β, IL-6 and IL-17 were extremely significantly different from those in self-healing group (P<0.01). It is demonstrated that ileal and colonic mucosal immune homeostasis of LIDHS model rat was destroyed, which manifested as hypersecretion of SIgA and secretion disorder of inflammatory cytokines. The treatment of YJP could back-regulate this phenomenon.

中图分类号:

姚万玲, 文艳巧, 华永丽, 纪鹏, 魏彦明. 郁金散对大肠湿热证大鼠回肠、结肠黏膜SIgA和炎性因子的影响[J]. 畜牧兽医学报, 2019, 50(10): 2147-2155.

YAO Wanling, WEN Yanqiao, HUA Yongli, JI Peng, WEI Yanming. Effects of Yujin Powder on SIgA and Inflammatory Cytokines in Ileal and Colonic Mucosa of Large Intestine Dampness-heat Syndrome Rat[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(10): 2147-2155.

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