秦川牛CD36基因多态性检测及其与肉用性状的相关性研究

doi:10.11843/j.issn.0366-6964.2014.07.005

摘要/Abstract

摘要：

旨在研究秦川牛脂肪酸转位酶基因（Cluster of differentiation 36，CD36）的多态性及其对秦川牛肉用性状的影响，以探索改善秦川牛肉用性状的分子育种方法。本研究选取288头同等饲养条件下的24月龄健康秦川牛母牛，采用DNA测序技术检测了CD36基因的多态性，并结合PCR-RFLP技术对所发现的多态位点进行基因型分型，然后将不同基因型与秦川牛肉用性状（背膘厚、眼肌面积、眼肌深度和肌内脂肪含量）进行关联性分析。结果表明，在CD36基因上发现4个多态位点，分别命名为SV1、SV2、SV3和SV4。连锁不平衡和单倍型分析表明，SV1-SV2为连锁位点，与SV3为强连锁，SV4与其他3个位点均为弱连锁，单倍型ITA属于优势单倍型(频率为60.4%)。关联分析结果表明，除SV4在眼肌深度指标中存在显著性差异外，单个多态位点的基因型之间和不同多态位点的组合基因型之间均在肌内脂肪含量指标中存在显著性或极显著性差异。其中，IITTAG型个体在肌内脂肪含量指标中显著低于IWTCAA型个体（P<0.05），极显著低于WWCCAA型个体（P<0.01）。结果提示，CD36基因对秦川牛肉用性状有显著的影响，可通过人工选择降低群体中IITTAG型个体的比例以改善肉用性状。

Abstract:

The aim of the study was to research polymorphism of fatty acid translocase gene (Cluster of differentiation 36，CD36) and analyze its effect on meat traits，then find molecular breeding methods of improving meat traits in Qinchuan cattle.In this study，288 24-month-old Qinchuan heifers under similar feeding conditions were randomly selected.Direct DNA sequencing and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) methods were used to genotype the polymorphisms of CD36 gene.The polymorphisms of bovine CD36 gene and its associations with meat traits (back fat thickness，eye muscle area，eye muscle depth and intramuscular fatty content) were analyzed.Four mutation sites were detected，named SV1，SV2，SV3，and SV4，respectively.Further，the linkage disequilibrium and haplotypes were analyzed among the four loci，suggested that SV1 and SV2 were complete linkage，SV1-SV2 and SV3 were strong linked，and SV4 was little linked with SV1-SV2 and SV3，respectively.Haplotype of ITA was dominant (with frequency of 60.4%).Except differentiation of eye muscle depth at SV4，the result of association analysis showed significant or extremely significant difference between different genotypes of single and combined sites in intramuscular fatty content in Qinchuan cattle，and the individuals with combined genotype IITTAG had a lower intramuscular fatty content than that of the individuals with combined genotype IITTAG (P<0.05) and combined genotype WWCCAA (P<0.01).As the result showed，CD36 gene had a significant effect on meat traits in Qinchuan cattle，and reducing the ratio of individuals with combined genotype IITTAG may be a method to improve meat traits.

中图分类号:

S813

张松，昝林森，王洪宝，王国庆，付常振，张亚冉，梅楚刚，邓冠群. 秦川牛CD36基因多态性检测及其与肉用性状的相关性研究[J]. 畜牧兽医学报, 2014, 45(7): 1060-1067.

ZHANG Song，ZAN Lin-sen，WANG Hong-bao，WANG Guo-qing，FU Chang-zhen，ZHANG Ya-ran，MEI Chu-gang，DENG Guan-qun. Association of CD36 Gene Polymorphism with Meat Traits in Qinchuan Cattle[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(7): 1060-1067.

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: https://www.xmsyxb.com/CN/10.11843/j.issn.0366-6964.2014.07.005

https://www.xmsyxb.com/CN/Y2014/V45/I7/1060

参考文献

［1］FEBBRAIO M，HAJJAR D P，SILVERSTEIN R L.CD36：a class B scavenger receptor involved in angiogenesis，atherosclerosis，inflammation，and lipid metabolism［J］.J Clin Invest，2001，108(6)：785-791.
［2］SILVERSTEIN R L，FEBBRAIO M.CD36，a scavenger receptor involved in immunity，metabolism，angiogenesis，and behavior［J］.Sci Signaling，2009，2(72)：re3.
［3］ABUMRAD N，HARMON C，IBRAHIMI A.Membrane transport of long-chain fatty acids：evidence for a facilitated process［J］.J Lipid Res，1998，39(12)：2309-2318.
［4］HARMON C M，ABUMRAD N A.Binding of sulfosuccinimidyl fatty acids to adipocyte membrane proteins：Isolation and ammo-terminal sequence of an 88-kD protein implicated in transport of long-chain fatty acids［J］. J Membrane Biol，1993，133(1)：43-49.
［5］周丽华，束刚，朱晓彤，等.肌肉脂肪酸转运膜蛋白及其相互关系［J］.中国生物化学与分子生物学报，2008，24(11)：992-1000.
［6］KOONEN D P Y，GLATZ J F C，BONEN A，et al.Long-chain fatty acid uptake and FAT/CD36 translocation in heart and skeletal muscle［J］.Mol Cell Biol Lipids，2005，1736(3)：163-180.
［7］YANG Y，CHEN M，LOUX T J，et al.Regulation of FAT/CD36 mRNA gene expression by long chain fatty acids in the differentiated 3T3-L1 cells［J］. Pediatric Surgery Int，2007，23(7)：675-683.
［8］AITMAN T J，GLAZIER A M，WALLACE C A，et al.Identification of CD36 (Fat) as an insulin-resistance gene causing defective fatty acid and glucose metabolism in hypertensive rats［J］.Nat Genet，1999，21(1)：76-83.
［9］FEBBRAIO M，ABUMRAD N A，HAJJAR D P，et al.A null mutation in murine CD36 reveals an important role in fatty acid and lipoprotein metabolism［J］.J Biol Chem，1999，274(27)：19055-19062.
［10］FEBBRAIO M，HAJJAR D P，SILVERSTEIN R L.CD36：a class B scavenger receptor involved in angiogenesis，atherosclerosis，inflammation，and lipid metabolism［J］. J Clin Invest，2001，108(6)：785-791.
［11］CHRISTIAENS V，VAN HUL M，LIJNEN H R，et al.CD36 promotes adipocyte differentiation and adipogenesis［J］.General Subjects，2012，1820(7)：949-956.
［12］PARK S Y，YUN Y，KIM I S.CD36 is required for myoblast fusion during myogenic differentiation［J］.Biochem Biophys Res Commun，2012，427(4)：705-710.
［13］BOKOR S，LEGRY V，MEIRHAEGHE A，et al.Single-nucleotide polymorphism of CD36 locus and obesity in European adolescents［J］.Obesity，2009，18(7)：1398-1403.
［14］HENI M，MSSIG K，MACHICAO F，et al.Variants in the CD36 gene locus determine whole-body adiposity，but have no independent effect on insulin sensitivity［J］. Obesity，2010，19(5)：1004-1009.
［15］CHEN C S Y，BENCH E M，ALLERTON T D，et al.Preference for linoleic acid in obesity-prone and obesity-resistant rats is attenuated by the reduction of CD36 on the tongue［J］. Am J Physiol-Regulat，Int Comp Physiol，2013，305(11)：R1346-R1355.
［16］J 萨姆布鲁克，D W 拉塞尔.分子克隆实验指南(上、下册)［M］.黄培堂，译.北京：科学出版社，2002.
［17］LOVE-GREGORY L，ABUMRAD N A.CD36 genetics and the metabolic complications of obesity［J］.Current Opin Clin Nutr Metab Care，2011，14(6)：527.
［18］LEPRETRE F，LINTON K J，LACQUEMANT C，et al.Genetic study of the CD36 gene in a French diabetic population［J］.Diabetes Metab，2004，30(5)：459-463.
［19］ARYA R，BLANGERO J，WILLIAMS K，et al.Factors of insulin resistance syndrome-related phenotypes are linked to genetic locations on chromosomes 6 and 7 in nondiabetic Mexican-Americans［J］.Diabetes，2002，51(3)：841-847.
［20］LEHMAN D M，ARYA R，BLANGERO J，et al.Bivariate linkage analysis of the insulin resistance syndrome phenotypes on chromosome 7q［J］.Hum Biol，2005，77(2)：231-246.
［21］MALHOTRA A，ELBEIN S C，NG M C Y，et al.Meta-analysis of genome-wide linkage studies of quantitative lipid traits in families ascertained for type 2 diabetes［J］.Diabetes，2007，56(3)：890-896.
［22］MA X，BACCI S，MLYNARSKI W，et al.A common haplotype at the CD36 locus is associated with high free fatty acid levels and increased cardiovascular risk in Caucasians［J］.Hum Mol Genet，2004，13(19)：2197-2205.
［23］刘正柱，董辉.影响牛肉品质的因素及牛肉嫩度的改善方法［J］.山东畜牧兽医，2013，34(8)：19-20.
［24］IBEAGHA-AWEMU E M，KGWATALALA P，ZHAO X.A critical analysis of production-associated DNA polymorphisms in the genes of cattle，goat，sheep，and pig［J］.Mamm Genome，2008，19(9)：591-617.
［25］CHEONG H，YOON D H，KIM L，et al.Growth hormone-releasing hormone (GHRH) polymorphisms associated with carcass traits of meat in Korean cattle［J］.BMC Genet，2006，7(1)：35.
［26］MOHR U，KOCZAN D，LINDER D，et al.A single point mutation results in A allele-specific exon skipping in the bovine α< sub> s1</sub>-casein mRNA［J］.Gene，1994，143(2)：187-192.
［27］ANDERSEN M，LENHARD B，WHATLING C，et al.Alternative promoter usage of the membrane glycoprotein CD36［J］.BMC Mol Biol，2006，7(1)：8.
［28］TARHDA Z，SEMLALI O，KETTANI A，et al.Three dimensional structure prediction of fatty acid binding site on human transmembrane receptor CD36［J］.Bioinform Biol Insights，2013，7：369.
［29］AN P，FREEDMAN B I，HANIS C L，et al.Genome-wide linkage scans for fasting glucose，insulin，and insulin resistance in the national heart，lung，and blood institute family blood pressure program evidence of linkages to chromosome 7q36 and 19q13 from meta-analysis［J］.Diabetes，2005，54(3)：909-914.
［30］LOVE-GREGORY L，SHERVA R，SUN L，et al.Variants in the CD36 gene associate with the metabolic syndrome and high-density lipoprotein cholesterol［J］.Hum Mol Genet，2008，17(11)：1695-1704.
［31］BONEN A，TANDON N N，GLATZ J F C，et al.The fatty acid transporter FAT/CD36 is upregulated in subcutaneous and visceral adipose tissues in human obesity and type 2 diabetes［J］.Int J Obes，2006，30(6)：877-883.
［32］GOUDRIAAN J R，DAHLMANS V E H，TEUSINK B，et al.CD36 deficiency increases insulin sensitivity in muscle，but induces insulin resistance in the liver in mice［J］. J Lipid Res，2003，44(12)：2270-2277.
［33］KOONEN D P Y，JACOBS R L，FEBBRAIO M，et al.Increased hepatic CD36 expression contributes to dyslipidemia associated with diet-induced obesity［J］. Diabetes，2007，56(12)：2863-2871.
［34］HU Y F，YEH H I，TSAO H M，et al.Impact of circulating monocyte CD36 level on atrial fibrillation and subsequent catheter ablation［J］.Heart Rhythm，2011，8(5)：650-656.
［35］GAUTAM S，BANERJEE M.The macrophage Ox-LDL receptor，CD36 and its association with type II diabetes mellitus［J］.Mol Genet Metab，2011，102(4)：389-398.
［36］REZAEI H B，DOOSTI M，AMINIAN M，et al.Compare the effect of eicosapentaenoic acid and oxidized low-density lipoprotein on the expression of CD36 and peroxisome proliferator-activated receptor gamma［J］.Iranian Biomed J，2013，17(2)：84.

[1]	王元清, 王兢, 朱波, 陈燕, 徐凌洋, 王泽昭, 张路培, 高会江, 李俊雅, 高雪. 基因组选配研究及其在畜禽育种中的应用[J]. 畜牧兽医学报, 2024, 55(1): 1-10.
[2]	张德安, 杨若渚, 刘杰, 刘德武, 邓铭, 柳广斌, 孙宝丽, 郭勇庆, 李耀坤. 饲喂青贮黄梁木代替青贮玉米川中黑山羊肝转录组的表达分析[J]. 畜牧兽医学报, 2024, 55(1): 232-244.
[3]	李超, 赵雪艳, 王永军, 王彦平, 任一帆, 李菁璇, 王怀中, 王继英, 宋勤叶. 莱芜猪和杜长大猪盲肠和结肠微生物菌群结构组成和功能分析[J]. 畜牧兽医学报, 2023, 54(12): 5033-5045.
[4]	李春艳, 张彦, 吕春荣, 邓卫东, 权国波. 褪黑素的抗氧化机制及其在哺乳动物精子冷冻保存中的应用研究进展[J]. 畜牧兽医学报, 2023, 54(11): 4468-4476.
[5]	郭艳丽, 李可强, 白少川, 王涛, 王德贺, 王麒, 李兰会. ALV-E的结构、活性调控以及对宿主功能的影响[J]. 畜牧兽医学报, 2023, 54(7): 2683-2691.
[6]	安宗麒, 占思远, 李利, 张红平. circRNA作为ceRNA调控畜禽重要经济性状的研究进展[J]. 畜牧兽医学报, 2023, 54(6): 2215-2222.
[7]	刘爽, 贺丽霞, 马钧, 冯雪, 杨梦丽, 汪书哲, 杨润军, 房希碧, 咸海龙, 王永康, 张路培, 马云. 固原黄牛遗传背景及其体尺指数、肉用指数分析[J]. 畜牧兽医学报, 2023, 54(6): 2376-2388.
[8]	杨苏坤, 董依萌, 王洪亮, 赵喜堂, 陈旭, 邢秀梅. 基于mtDNA和Y染色体基因片段的塔河马鹿种公鹿遗传多样性分析[J]. 畜牧兽医学报, 2023, 54(6): 2402-2413.
[9]	马克岩, 韩金涛, 白雅琴, 李讨讨, 马友记. 基于简化基因组测序的永登七山羊遗传多样性分析[J]. 畜牧兽医学报, 2023, 54(5): 1939-1950.
[10]	王开明, 喻宗岗, 徐雪莉, 艾妮妮, 李昕瞳, 何俊, 陶灯, 张硕, 马海明, 张跃博. 干扰Mtmr3基因对C2C12细胞增殖与分化的影响[J]. 畜牧兽医学报, 2023, 54(4): 1478-1489.
[11]	韩露露, 韩德平, 赵启南, 刁其玉, 崔凯. miRNA介导应激幼畜肠道损伤的研究进展[J]. 畜牧兽医学报, 2023, 54(3): 877-888.
[12]	刘铃, 王丹丹, 崔凯, 马月辉, 蒋琳. 猪繁殖与呼吸综合征抗病育种研究进展[J]. 畜牧兽医学报, 2023, 54(2): 434-442.
[13]	杨小耿, 张慧珠, 李键, 向华, 何翃闳. DNA甲基化在哺乳动物卵母细胞和早期胚胎发育中的研究进展[J]. 畜牧兽医学报, 2023, 54(2): 443-450.
[14]	刘继强, 郝晓东, 武丽娜, 廖诗莹, 冯羿方, 弥世荣, 刘燊, 刘建, 张龙超. 全基因组SNP分型技术在畜禽遗传育种研究中的应用[J]. 畜牧兽医学报, 2022, 53(12): 4123-4137.
[15]	邹惠影, 李俊良, 朱化彬. 引导编辑系统的研究与应用进展[J]. 畜牧兽医学报, 2022, 53(11): 3721-3730.