畜牧兽医学报 ›› 2015, Vol. 46 ›› Issue (10): 1733-1740.doi: 10.11843/j.issn.0366-6964.2015.10.005

• 遗传繁育 • 上一篇    下一篇

绒山羊次级毛囊生长调控基因的筛选

李晓燕#,张璐#,王乐乐,邬娇,宋慧子,涂藩镜,张燕军*   

  1. (内蒙古农业大学动物科学学院,动物遗传育种与繁殖自治区重点实验室,呼和浩特 010018)
  • 收稿日期:2015-03-17 出版日期:2015-10-23 发布日期:2015-10-23
  • 通讯作者: 张燕军,博士,副教授,主要从事绒山羊遗传育种研究,E-mail:imauzyj@163.com
  • 作者简介:李晓燕(1990-),女,内蒙古锡林郭勒盟人,硕士生,主要从事绒山羊遗传育种研究,E-mail:lxy12898159@126.com;张璐(1989-),女,内蒙古四子王旗人,硕士生,主要从事绒山羊遗传育种研究,E-mail:zl63412732@163.com。李晓燕和张璐为并列第一作者
  • 基金资助:

    国家自然科学基金项目(31260539;31201773);内蒙古农业大学优秀青年科学基金 (2014XYQ-1 );内蒙古农业大学学生科技创新基金项目(2014-1 );国家现代绒毛用羊产业技术体系建设项目(CARS-40-05)

Selection of Relative Gene during Secondary Hair Follicle Growth in Cashmere Goat

LI Xiao-yan#,ZHANG Lu#,WANG Le-le,WU Jiao,SONG Hui-zi,TU Fan-jing,ZHANG Yan-jun*   

  1. (Inner Mongolia Key Laboratory of Animal Genetics,Breeding and Reproduction,College of Animal Science,Inner Mongolia Agricultural University,Hohhot 010018,China)
  • Received:2015-03-17 Online:2015-10-23 Published:2015-10-23

摘要:

旨在筛选绒山羊次级毛囊生长调控基因。本研究以遗传背景相似,有次级毛囊的绒山羊和无次级毛囊的奶山羊为试验动物,利用Illumina Hiseq2000 对4只绒山羊与4只奶山羊3、6、9、12月的32个皮肤样品进行RNA-Seq测序。测序共获得38G数据,共筛选出20 780个基因。差异表达基因分析表明,绒山羊与奶山羊之间在不同时期共有2 409个差异表达基因,其中上调差异表达基因1 175个,下调差异表达基因1 234个, 绒山羊之间在不同生长时期差异表达基因共550个,其中上调差异表达基因302个,下调差异表达基因248个。GO功能注释分类显示,差异表达基因主要在细胞与细胞部分、细胞器部分、结合、催化、生物调控、细胞过程、代谢过程等功能富集。 KEGG pathway分析发现,42个差异表达基因富集在与毛囊生长相关的WNTSHHTGFβTNFMAPKNotch信号通路中,由此推测其可能为次级毛囊生长相关基因。本研究为深入开展次级毛囊生长相关基因功能验证提供丰富的资源,为揭示次级毛囊生长调控机制提供重要基础,为绒毛产量及品质的提升提供理论依据。

Abstract:

In order to select the genes which regulate the growth of secondary hair follicle in cashmere goat.This study choose cashmere goat and dairy goats to be the material.Because these 2 goats get the similar genetic background,but significant differences traits of the secondary follicle.An Illumina Hiseq2000 platform was used to sequence the 32 skin samples from 4 cashmere goat and 4 dairy goat on March,June,September and December.The Sequencing obtained 38G data,check out 20 780 goats genes.We identified 2 409 differentially expressed genes between cashmere goat and dairy goat in the same period,1 175 cashmere goat genes were up regulated compared with dairy goat,1 234 were down regulated.And there were a total of 550 differentially expressed genes between different growth periods in cashmere goat,302 cashmere goat genes were up regulated,248 were down regulated.GO functional annotations showed that the main differentially expressed genes appear in the cells and cell parts,organs parts,binding,biological regulation,cellular process.KEGG pathway analysis found that 42 differentially expressed genes appear in WNTSHHTGF βTNFMAPK and Notch signaling pathways which intimately associated with hair follicle growth.This study provided valuable information to verify secondary follicle growth related gene function,provided an important basis to reveal the secondary follicle growth mechanism and provided the theory to increase production and quality of cashmere.

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