羊驼皮肤中可溶性鸟苷酸环化酶的表达差异

doi:10.11843/j.issn.0366-6964.2013.07.010

畜牧兽医学报 ›› 2013, Vol. 44 ›› Issue (7): 1057-1062.doi: 10.11843/j.issn.0366-6964.2013.07.010

羊驼皮肤中可溶性鸟苷酸环化酶的表达差异

张俊珍，范瑞文，杨姗姗，石占全，姬凯元，董常生^*

（山西农业大学动物科技学院，太谷030801）

收稿日期:2013-03-03 出版日期:2013-07-23 发布日期:2013-07-23
通讯作者: 董常生，教授，主要从事生物工程研究,E-mail: cs-dong@sxau.edu.cn
作者简介:张俊珍（1972-），男，山西偏关人，讲师，硕士，主要从事动物生产学研究，Tel:0354-6288335,E-mail:junzhenzhang@163.com
基金资助:
山西省科技攻关项目(20120311034-2);国家自然科学基金（30671512）

The Different Expression of Soluble Guanylate Cyclase in Alpaca Skin

ZHANG Jun-zhen, FAN Rui-wen, YANG Shan-shan, SHI Zhan-quan, JI Kai-yuan,DONG Chang-sheng^*

(College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu 030801，China)

Received:2013-03-03 Online:2013-07-23 Published:2013-07-23

摘要/Abstract

摘要：

采用免疫组织化学、实时荧光定量PCR和Western blotting对可溶性鸟苷酸环化酶（sGC）在棕色和白色羊驼皮肤中的表达差异进行了研究, 以探讨sGC在羊驼皮肤中的作用。实时荧光定量PCR结果显示，sGC在棕色羊驼皮肤中的相对表达量较低，是其在白色羊驼皮肤中的表达量的0.16倍，且差异极显著（P<0.01）；Western blotting结果显示，在羊驼皮肤总蛋白中含有与兔多克隆抗体发生免疫阳性反应的条带，在棕色皮肤中的反应阳性弱于白色皮肤，且差异极显著（P<0.01）；免疫组织化学结果表明，sGC免疫阳性主要分布在白色羊驼皮肤毛囊的毛球上方细胞及细胞间质，而在棕色羊驼皮肤毛囊中，sGC免疫阳性主要分布在毛球底部和外根鞘细胞及细胞间质。结果提示sGC参与羊驼毛色形成。

Abstract:

To investigate the function of soluble guanylate cyclase (sGC) in alpaca skin and coat color regulation, differential expression of sGC in skin of brown versus white alpacas was examined using quantitative real-time PCR(qRT-PCR), Western blotting and immunohistochemistry．Expression of sGC mRNA was lower (0.16 fold) in skin samples from brown alpacas relative to expression in skin of white alpacas with significant difference (P<0.01). Using a rabbit polyclonal sGC antibody, an immune reactive band corresponding to sGC was detected by Western blotting analysis in skin samples from white and brown alpacas, showing a weaker signal detected in the samples from brown alpacas with significant difference (P<0.01). The results of immunohistochemistry revealed that sGC was localized in the cytoplasm and intercellular substance of upper hair papilla in hair follicles of alpacas with white hair color, but was localized in ytoplasm and intercellular substance of lower hair bulb and outer root sheath cells in hair follicles of alpacas with brown hair color. Observed differential expression and localization of sGC in skin of white versus brown alpacas suggests a potential role in hair color regulation.

中图分类号:

张俊珍，范瑞文，杨姗姗，石占全，姬凯元，董常生. 羊驼皮肤中可溶性鸟苷酸环化酶的表达差异[J]. 畜牧兽医学报, 2013, 44(7): 1057-1062.

ZHANG Jun-zhen, FAN Rui-wen, YANG Shan-shan, SHI Zhan-quan, JI Kai-yuan,DONG Chang-sheng. The Different Expression of Soluble Guanylate Cyclase in Alpaca Skin[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(7): 1057-1062.

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羊驼皮肤中可溶性鸟苷酸环化酶的表达差异

The Different Expression of Soluble Guanylate Cyclase in Alpaca Skin

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