性腺激素对山羊子宫内膜上皮细胞激素受体表达的影响

畜牧兽医学报

性腺激素对山羊子宫内膜上皮细胞激素受体表达的影响

屈延延，齐雪峰^*，南志春，苏运芳，李义，赵献军，王爱华，靳亚平

（西北农林科技大学动物医学院农业部家畜生殖内分泌与胚胎工程重点开放实验室，杨凌 712100）

收稿日期:2012-03-19 出版日期:2013-01-23 发布日期:2013-01-23
通讯作者: 齐雪峰，讲师，博士，E-mail：yxyan2002@126.com
作者简介:屈延延(1986-)，女，陕西洛川人，硕士生，主要从事家畜生殖内分泌学与免疫学相关研究，E-mail: qyy19860518@126.com
基金资助:
国家自然科学基金（30901089）；国家博士点基金资助项目（20090204120011）；西北农林科技大学“青年学术骨干支持计划”（Z111020902）

Effect of Sex Hormone on the Cells Expression of Hormone Receptor for Goat Endometrial Cells

QU Yan-yan, QI Xue-feng^*, NAN Zhi-chun, SU Yun-fang, LI Yi, ZHAO Xian-jun, WANG Ai-hua, JIN Ya-ping

（Key Laboratory of Animal Reproductive Endocrinology and Embryo Biotechnology of Agriculture Ministry of China,College of Veterinary Medicine, Northwest A ＆ F University,Yangling 712100, China）

Received:2012-03-19 Online:2013-01-23 Published:2013-01-23

摘要/Abstract

摘要：

为探讨性腺激素对山羊子宫内膜上皮细胞激素受体表达的影响。本研究构建永生化山羊子宫内膜上皮细胞(EEC)与基质细胞(ESC)体外培养研究模型，通过细胞免疫荧光技术检测性腺激素E₂或/和 P₄对单独培养EEC中PR与ER表达水平的调节作用以及ESC对该作用的影响。结果显示：与未加激素对照组相比，单独添加P₄或与E₂同时加入均可显著抑制单独培养EEC中PR与ER的表达 (P＜0.05)；而单独添加E₂可轻微促进2种激素受体表达水平。在添加有ESC条件培养液的EEC培养模式中，E₂单独作用对EEC中PR与ER表达水平无显著影响；P₄单独或与E₂共同作用下，EEC中PR与ER表达水平均较未加激素对照组显著降低(P＜0.05)。性腺激素对单独培养及与ESC共培养模式下的EEC中PR与ER表达水平的调节作用相似，P₄对山羊EEC中PR与ER表达水平具有显著抑制作用。

Abstract:

To study the effect of sex gland hormones on the cells expression of hormone receptor to endometrial cells of goats, immortalization model of goats endometrial epithelial cells (EEC) and stromal cells (ESC) in vitro culture were established, the different levels and combinations of estradiol(E₂) and progesterone(P₄) were added in medium, without or with ESC culture medium together, PR and ER expression levels in EEC were determined by immunofluorescence technique.The results showed that compared with control group, when added P₄ alone or together with E₂, the level of both PR and ER in EEC were significantly decreased(P＜0.05), but they were slightly enhanced by E₂ adding alonely. In the EEC culture without ESC contioned medium, the expression level of PR and ER in EEC had no significant change by E₂ adding alonely.Compared with control group, the expression level of PR and ER in EEC significantly decreased by P₄ adding alonely or combined action with E₂(P＜0.05). The results indicated that when the sex hormones in coculture mode alone or together with ESC culture medium, the regulation of PR and ER in EEC were similar, and they were repressed significantly by P₄ alone.

中图分类号:

S827
S814.1

屈延延，齐雪峰，南志春，苏运芳，李义，赵献军，王爱华，靳亚平. 性腺激素对山羊子宫内膜上皮细胞激素受体表达的影响[J]. 畜牧兽医学报, doi: .

QU Yan-yan, QI Xue-feng, NAN Zhi-chun, SU Yun-fang, LI Yi, ZHAO Xian-jun, WANG Ai-hua, JIN Ya-ping. Effect of Sex Hormone on the Cells Expression of Hormone Receptor for Goat Endometrial Cells[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, doi: .

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参考文献

［1］ROBERTS R M, CHEN Y, EZASHI T, et a1. Interferons and the maternal-conceptus dialog in mammals［J］. Semin Cell Dev Biol, 2008,19:170-177.

［2］KING A E, CRITCHLEY H O D. Oestrogen and progesterone regulation of inflammatory processes in the human endometrium［J］. J Steroid Biochem, 2010, 120:116-126.

［3］WANG H B, LU S H, LIN Q X, et al. Reconstruction of endometrium in vitro via rabbit uterine endometrial cells expanded by sex steroid［J］. Fertil Steril, 2010, 93(7):2385-2395.

［4］PARIA B C, LIM H, DAS S K, et a1. Molecular signaling in uterine receptivity for implantation［J］. Semin Cell Dev Biol, 2000, 11(2):67-76.

［5］SLAYDEN O D, BRENNER R M. Hormonal regulation and localization of estrogen, progestin and and estrogen receptors in the endometrium of nonhuman primates: effects of progesterone receptor antagonists［J］. Arch Histol Cytol, 2004, 67(5):393-409.

［6］BOMBAIL V, MACPHERSON S, CRITCHLEY H O, et al. Estrogen receptor related beta is expressed in human endometrium throughout the normal menstrual cycle［J］. Hum Reprod, 2008, 23:2782-2790.

［7］张鸿波, 秦国嵩, 刘云海, 等. 雌激素和孕激素对不同发情方式小鼠子宫内膜中孕激素受体分布的影响［J］.中国实验动物学报, 2009, 17(3):206-209.

［8］SIMMONS C D, PABONA J M P, ZENG Z, et al.Response of adult mouse uterus to early disruption of estrogen receptor-α signaling is influenced by Krüppel-like factor 9［J］. J Endocrinol, 2010, 205:147-157.

［9］HE Y L, WU Y H, HE X N, et a1. An immortalized goat mammary epithelial cell line induced with human telomerase reverse transcriptase (hTERT) gene transfer［J］. Theriogenology, 2009,71:1417-1424.

［10］ZHAO C F, HU H Y, MENG L, et a1. Immortalization of bovine mammary epithelial cells alone by human telomerase reverse transcriptase［J］. Cell Biol Int, 2010,34:579-586.

［11］盛红霞，吴庆侠，靳亚平，等. 山羊子宫内膜基质细胞永生化研究［J］.畜牧兽医学报, 2009,40(6):818-823.

［12］吴庆侠, 王爱华, 司永嘉, 等. 山羊子宫内膜上皮细胞转染pCI-neo-hTERT质粒后的永生化［J］.中国兽医学报, 2010, 30(2):228-232.

［13］陈秀荔, 靳亚平, 张彦明. 分离培养兔子宫内膜细胞的鉴定及性激素对子宫内膜间质细胞形态的影响［J］.畜牧兽医学报, 2006, 37(11):1226-1231.

［14］RUSSO L A, PEANO B J, TRIVEDI S P, et al. Regulated expression of matrix metalloproteinases, inflammatory mediators, and endometrial matrix remodeling by 17 betaestradiol in the immature rat uterus［J］. RB & E, 2009, 124(7):1-19.

［15］李莹, 侯丽辉, 吴效科, 等. 着床过程中子宫与滋养层的相互作用［J］.中国妇幼健康研究, 2008, 19(3):266-268.

［16］FRANCHI A, ZARET J, ZHANG X, et al. Expression of immunomodulatory genes, their protein products and specific ligands/ receptors during the window of implantation in the human endometrium［J］. Mol Hum Reprod, 2008, 14:413-421.

［17］AYATOLLAHI M, GERAMIZADEH B, YAZDANI M, et a1.Effect of the immunoregulatory cytokines on successful pregnancy depends upon the control of grafl rejection mechanisms［J］. Transplant Proc, 2007, 39(1):244-245.

［18］TSUJI Y, TAMAOKI T H, HASEGAWA A, et a1. Expression of interleukin-18 and its receptor in mouse ovary［J］. Am J Reprod Immunol, 2001, 46:349-357.

［19］HELDRING N, PIKE A, ANDERSSON S, et al. Estrogen receptors: how do they signal and what are their targets［J］. Physiol Rev, 2007, 87:905-931.

［20］GRIFFITH J S, RODGERS A K, SCHENKEN R S. In vitro models to study the pathogenesis of endometriosis［J］. Reprod Sci, 2010, 17(1): 5-12.

［21］DYAO L, AGOULNIK A I, COOKE P S, et a1. Relative roles of the epithelial and stromal tissue compartment(s) in mediating the actions of relaxin and estrogen on cell proliferation and apoptosis in the mouse lower reproductive tract［J］. Ann N Y Acad Sci, 2009, 1160:121-129.

［22］SINGH H, NARDO L, KIMBER S J, et al. Early stages of implantation as revealed by an in vitro model［J］. Reproduction, 2010, 139(5): 905-914.

［23］PIERRO E, MINICI F, ALESIANI O, et al. Stromal-epithelial interactions modulate estrogen responsiveness in normal human endometrium［J］. Biol Reprod, 2001,64(3):831-838.

［24］LESSEY B A. Assessment of endometrial receptivity［J］.Fertil Steril, 2011,96(3):522-529.

［25］BAZER F W, WU G, SPENCER T E, et al. Novel pathways for implantation and establishment and maintenance of pregnancy in mammals ［J］. Mol Hum Reprod, 2010,16(3):135-152.

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