畜牧兽医学报 ›› 2010, Vol. 41 ›› Issue (7): 811-815.doi:

• 遗传繁育 • 上一篇    下一篇

淫羊藿苷诱导mESCs分化为心肌样细胞的研究

相荣科1，2，穆祥1，赵菊2，吴波2，刘笑然2，张涛1，高建明1，2*   

  1. 1. 北京市兽医学（中医药）重点实验室，北京102206；2. 北京农学院动物科学技术学院，北京 102206
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-07-20 发布日期:2010-07-20
  • 通讯作者: 高建明

Study on Icariin Inducing Mouse Embryonic Stem Cells to Differentiate into Cardiomyogenic Cells

XIANG Rong-ke1,2, MU Xiang1, ZHAO Ju2, WU Bo2,LIU Xiao-ran2, ZHANG Tao1, GAO Jian-ming1,2*   

  1. 1. Beijing Key Laboratory of TCVM, Beijing 102206, China; 2. Animal Science and Technology College, Beijing University of Agriculture, Beijing 102206, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-07-20 Published:2010-07-20

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摘要： 本研究探讨了淫羊藿苷 （Icariin，ICA ）对小鼠胚胎干细胞（Mouse embryonic stem cells，mESCs）定向分化为心肌样细胞的效果。试验以mESCs分化培养液为空白对照组，分别添加淫羊藿苷10-6 mol·L-1（ICA 1）、10-7 mol·L-1（ICA 2）、10-8 mol·L-1（ICA 3）3个剂量为试验组，对诱导后培养4周的mESCs采用免疫细胞化学法鉴定诱导后细胞中肌动蛋白T（cTnT）的表达，并计算心肌样细胞分化率；采用RTPCR鉴定诱导后mESCs中心肌特异性基因MLC-2v、α-MHC、β-MHC、 Nkx2.5、GATA-4的表达。结果在诱导后细胞中有心肌特异性蛋白cTnT表达；ICA 2组分化率显著高于对照组和其他剂量组；且诱导后细胞有心肌特异性基因MLC-2v、α-MHC、β-MHC、 Nkx2.5、GATA-4表达。结果表明，ICA可在体外诱导mESCs定向分化为心肌样细胞，10-7 mol·L-1 ICA为最佳诱导浓度。

Abstract: The experiment was conducted to study the effect of Icariin(ICA) on inducing mouse embryonic stem cells (mESCs) differentiating into cardiomyogenic cells. mESCs were induced by ICA (terminal concentration were 10-6 mol·L-1 (ICA1),10-7 mol·L-1 (ICA2),10-8 mol·L-1 (ICA3)). Meanwhile, a blank control group was set up. After induction, mESCs were cultured for 4 weeks and cardiomyogenic cell percentage was worked out, the expression of specific proteins of cardiac troponin T(cTnT) was detected by immunohistochemical method, and the mRNA expression of MLC-2v,α-MHC,β-MHC, Nkx2.5 and GATA-4 in myocardiocytes were examined by RT-PCR analysis. These results indicated that the expression of MLC-2v,α-MHC,β-MHC, Nkx2.5, GATA-4 were positive, and the mESCs were differentiated into cardiomyogenic cells. The difference of cardiomyogenic cell percentage was significantly higher in ICA2 group than that in other groups. These results suggested that ICA could induce mESCs differentiating into cardiomyogenic cells in vitro, and 10-7 mol·L-1 ICA was the best concentration.

ISSN 0366-6964
CN 11-1985/S
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