Abstract: This experiment was conducted to screen differential expressed genes of bovine preimplantation embryos derived from in vitro fertilization and make differential expression analysis. Bovine oocytes, 8 cell stage embryos and blastocysts derived from in vitro fertilization were selected for mRNA differential display in this study. Four differential expression genes in bovine oocytes and preimplantation embryos in total were screened. The sequencing and alignment results indicated that 4 differential expression genes were highly homologous with HMGXB4, Dnajc8, RIMS2 and RPL31. The results of RT-PCR detection showed that genes expression of HMGXB4, Dnajc8, RIMS2 and RPL31 in bovine oocytes and preimplantation embryos were temporaldifferential, and the reasons might be related with participating in different physiological activities.