Abstract: This study was conducted to test the efficacy of calcium amino acid chelate as an additive on health status and milk quality in postpartum dairy cows. Forty-five postpartum dairy cows were alternately assigned to three groups based on calving date. Cows in control group were fed conventional diet, calcium amino acid chelate solution was given to dairy cows in the second group based on conventional diet, calcium amino acid chelate with magnesium hypophosphate oral solution was given to the third group based on conventional diet. Blood samples were collected after calving. Plasma samples and milk samples were collected to analyze related parameters. Compared with the control, the concentration of plasma total calcium in the second group were increased, the concentration of plasma PTH were decreased. Incidence of subclinical hypocalcemia and retained placenta in the second group were lower than that in control group, and the second group had higher milk protein than control group. The concentration of plasma total calcium, plasma magnesium and the milk protein content in the third group were increased than that in control group. Incidence of subclinical hypocalcemia and retained placenta in the third group were lower than that in control group. The concentration of plasma CT in the third group were increased at 7 d postpartum very significantly than that in control group, and the incidence of hypomagnesaemia was very significant lower than that in control group. Hence, calcium amino acid chelate oral solution treatment was beneficial in improving the health status and milk quality in postpartum dairy cows, especially the oral solution which was added magnesium hypophosphate.