Abstract: This experiment was conducted to investigate effects of oxidized oil and antioxidant supplementation in breeder hen diet on reproduction performance of the breeders and maternal effects to the offspring. A total of 200 Cobb-Avian 48, 29 weeks old broiler breeder hens were assigned randomly to 5 groups with 5 replicates of 8 birds. Each group was fed one of the following diets; 2% fish oil (FO), 2% oxidized oil（OFO）, 2% OFO+100 mg·kg^-1 VE, 2% OFO+198 mg·kg^-1 EMQ, 2%OFO+100 mg·kg^-1VE+198 mg·kg^-1 EMQ, the progeny broilers were fed the same diet. The results showed that: (1) Inclusion of OFO in the breeder hens′ diet reduced laying percentage（P<0.05） and egg production（P<0.05）of the breeders; (2) OFO in the breeder hens′ diet reduced neonatal offspring chick weight of the breeders（P<0.05）; (3) Offspring chicks from the dam feeding oxidized oil had shorter jejunum villus height (P<0.01) and thinner jejunum villus width (P<0.01); (4) OFO in the breeder hens′ diet reduced Body weight gain of 0-7 d (P<0.01), body weight at 7 d (P<0.05), feed intake of 7-14 d (P<0.01) and body weight at 14 d (P<0.01) of the offspring broilers. These data indicated that oxidized oil had obvious toxicity to the breeder hens, not only reduced the reproduction performance of the breeder hens, but also decreased the neonatal offspring chick body weight, injuried the morphology of the jejunum of offspring chicks, resulted in decreased offspring performance.