Abstract: In order to study the effects of Botanical activity flour of garlic bacterium substance (GBS) with four doses on performance and hormone secretion of Yangzhou geese, and provide theoretical basis and application consult for the development and utilization, 240 one-day-old Yangzhou geese were allocated to five groups,including control group and trial group 1,2,3,4. The control group was fed with basical diets，each trial group was fed with basical diets added with 0.25,0.50,1.00,2.00 g·kg^-1 GBS,respectively. The results showed that, compared with control group, the body weight gain of 1-60-day-old trial groups increased by 5.91% (P<0.05), 8.18% (P<0.01),13.64%(P<0.01),13.64%(P<0.01); the feed intake in trial group 4 increased by 8.79% (P<0.01); the feed conversion ratio in trial group 2,3 decreased by 7.55%(P<0.05), 9.37%(P<0.01). Crude protein of brisket in trial group 2,4 increased by 22.88%(P<0.05), 17.81%(P<0.05); Crude fat of brisket in each trial group decreased by 22.82%(P<0.05), 31.21%(P<0.05), 44.63%(P<0.05),33.22%(P<0.05). Serum T3 in trial group 3,4 increased by 11.76%(P<0.01),14.38%(P<0.01); serum T4 in each trial group increased by 7.75%(P<0.01),14.06%(P<0.01),14.30% (P<0.01), 66.52%(P<0.01); serum Glu in trial group 1,3,4 decreased by 14.78%(P<0.01), 14.45% (P<0.01),16.05%(P<0.01). The average level of Ins after feeding in 3 h trial group 2，3，4 decreased by 14.85%(P<0.01), 12.54%(P<0.01),23.27% (P<0.01); the average level of serum glucose in trial group 1,3,4 decreased by 7.96%(P<0.01),6.80%(P<0.01),11.30% (P<0.01). It had a encourage effect on performance and hormone secretion of Yangzhou geese by supplemented with GBS on their Goose die, and four doses in this trial, the effects of fed basical diets added with GBS 2.00 g·kg^-1 is the best.