Abstract: The research was conducted to construct and identify the support plasmids and minigenome of Peste des petits ruminants virus (PPRV) in order to lay a foundation for the establishment of a reverse genetic system for rescue of PPRV. Support plasmids of pCIN, pCIP and pCIL according to the PPRV isolate, China/Tib/07, were constructed and functions were identified by IFA and RTPCR after transfection. At the same time, the two end sequences of the genome of the PPRV isolate, along with the reporter gene of eGFP were amplified, then the 3 fragments were connected by overlapPCR and cloned into the transcription vector, pOLTV5, the minigenome was constructed and then cotransfected with the support virus and the 3 support plasmids respectively. As a result, the 3 support plasmids were identified to be correct and the reporter gene of eGFP expressed when the minigenome transfected with either the support virus or the 3 support plasmids. In conclusion, support plasmids of the N, P and L genes and minigenome of the PPRV were constructed successfully. Furthermore, the 3 plasmids worked well with the minigenome, which indicated that the 3 plasmids can work as the support plasmids for the study of reverse genetic operations of China/Tib/07.