Abstract: In August 2009, low produce peak was observed by farm owner in Hyline layer chicken flock at Tai′an in Shandong province. The rate of produce only was 60%70%, and the mortality was up to 20% at age of 31week. The ill chickens were diagnosed as avian leukosis virus subgroup J infection by gross observation, pathology, PCR and immunohistochemistry. Except myelocytomas, fibrosarcomas, histiocytic sarcomas, intestinal adenocarcinoma and hemangioendothelioma were also observed in same or different organs. Fibrosarcomas exist single in ill chicken, and the other tumors were concurrent with myelocytomas. The histopathology detail characters of tumors were described in this paper. The etiology and mechanism of transmmition and tumor spectrum expended of ALVJ need to be further studied.