大豆黄酮和染料木素对体外培养奶牛乳腺上皮细胞增殖及抗氧化水平的影响

畜牧兽医学报 ›› 2008, Vol. 39 ›› Issue (11): 1517-1522.doi:

大豆黄酮和染料木素对体外培养奶牛乳腺上皮细胞增殖及抗氧化水平的影响

刘春龙^1,2，李忠秋³ ，张帆¹ ，姜文博¹，徐岩¹ ，单安山¹*

1. 东北农业大学动物营养研究所，哈尔滨 150030； 2. 中国科学院东北地理与农业生态研究所黑土生态重点实验室，哈尔滨 150081；3. 黑龙江省农业科学院畜牧研究所，哈尔滨 150086

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2008-11-24 发布日期:2008-11-24
通讯作者: 单安山

Effect of Daidzein and Genistein on Proliferation and Antioxidation of Mammary Epithelial Cell of Dairy Cow in vitro

LIU Chun-long^1,2 ，LI Zhong-qiu³，ZHANG Fan¹，JIANG Wen-bo¹ ，
XU Yan¹，SHAN An-shan¹*

1. Animal Nutrition Institute,Northeast Agricultural University, Harbin 150030, China; 2. Heilongjiang Key Laboratory of Blacksoil Ecology,Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin 150081,China;3. Animal Science Research Centre,Heilongjiang Academy of Agricultural Research,Harbin 150086,China

Received:1900-01-01 Revised:1900-01-01 Online:2008-11-24 Published:2008-11-24

摘要/Abstract

摘要： 通过体外细胞培养的方法探讨大豆黄酮（Daidzein，Da）和染料木素（Genistein，Ge）对奶牛乳腺上皮细胞增殖及抗氧化水平的影响。试验分空白对照组、10 ng/mL雌二醇（E2）组、不同浓度Da和Ge（1、10、100、1 000 ng/mL）组，培养72 h后，采用MTT法检测细胞增殖情况。结果表明，10、100 ng/mL Ge组和100、1 000 ng/mL Da组同空白对照组比较可显著促进奶牛乳腺上皮细胞的增殖（P＜0.05），但均显著低于E2组（P＜0.05）。另取处于对数生长期的乳腺上皮细胞加入不同浓度Da和Ge（1、10、100、1 000 ng/mL）继续培养24 h，检测细胞培养液中总超氧化物歧化酶（T-SOD）、谷胱甘肽过氧化物酶（GSH-PX）活性、丙二醛（MDA）、一氧化氮（NO）含量。结果表明，100、1 000 ng/mL Da组及100、1 000 ng/mL Ge组较空白组显著提高了T-SOD活性（P＜0.05），显著降低了MDA含量（P＜0.05）；100、1 000 ng/mL Da组及10、100、1 000 ng/mL Ge组显著提高了NO含量（P＜0.05）；1 000 ng/mL Da组及100、1 000 ng/mL Ge组显著提高了GSHPX活性（P＜0.05）。可见，Da和Ge在一定浓度范围内均有促进奶牛乳腺上皮细胞增殖及抗氧化水平提高的作用。

Abstract: Effect of Daidzein and Genistein on the proliferation and antioxidation of mammary epithelial cell of dairy cow were determined in vitro. The experiments were assigned into 9 groups(blank,1,10,100,1 000 ng/mL Daidzein and Genistein).According to MMT,the proliferation experiment results showed that 10,100 ng/mL Genistein and 100,1 000 ng/mL Daidzein significantly improved dairy cow mammary epithelial cell proliferation(P＜005),but all weaker than 10 ng/mL E₂group (P＜0.05) after 72 h cultured. In the other experiment,the mammary epithelial cell during the logarithm growing period were collected after 24 h cultured to measured the TSOD,GSH-PX activity,MDA,NO content.Results showed that 100,1 000 ng/mL Daidzein and 100,1 000 ng/mL Genistein significantly increased the T-SOD activites and decreased MDA concentration (P＜0.05). 100,1 000 ng/mL Daidzein and 10,100, 1 000 ng/mL Genistein significantly increased NO concentration(P＜0.05).1 000 ng/mL Daidzein and 100,1 000 ng/mL Genistein significantly increased the GSH-PX activites (P＜0.05).It was proved that proper levels of Daidzein and Genistein can advance the proliferation and antioxidation of mammary epithelial cell of dairy cow.

刘春龙;李忠秋;张帆;姜文博;徐岩;单安山. 大豆黄酮和染料木素对体外培养奶牛乳腺上皮细胞增殖及抗氧化水平的影响[J]. 畜牧兽医学报, 2008, 39(11): 1517-1522.

LIU Chun-long;;LI Zhong-qiu;ZHANG Fan;JIANG Wen-bo;XU Yan;SHAN An-shan. Effect of Daidzein and Genistein on Proliferation and Antioxidation of Mammary Epithelial Cell of Dairy Cow in vitro[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2008, 39(11): 1517-1522.

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