Abstract: Appearance, biochemical and molecular genetic markers were applied into the process of Mohair production using Angora goat gradebreed Jianchang black goat in order to establish crossbreeding program. Angora goat, Jianchang black goat and their generations F₁, F₂, F₃ and H were all studied in this research. The results of appearance genetic markers research showed that the individuals of white wool in F₂， F₃ and H generations were 93.5% ，100% and 100% respectively. The percentages of homogeneity and nearly homogeneity individuals in F₂ ，F₃ and H generations were 27.7%，70.2% and 65.3% respectively. The Mohaw yields of adult mals and females increased with the increased of generations ，but which not reached marked difference level between F₃ with H and F₂，H with F₃(P＞0.05). The average adult body weights increased in F₁ and F₂ generation，but is not marked in F3 generation(P＞0.05)， declined in H generation(P>0.05). The study of biochemical genetic marker demonstrated that average gene heterozygosity and gene consistency in Agora goat, Jianchang black goat, F₃ and H generations were 0.113 1 and 0.886 9, 0.062 1 and 0.937 9， 0.110 3 and 0.889 7, 0.110 4 and 0.889 6 respectively. The genetic distance is 0.003 9 and 0.004 0, the genetic similarity coefficient is 0.996 1 and 0.996 0 between Angora goats and F₃ and H generation. DNA fingerprinting technique as molecular genetic marker was also used in the research. The results revealed the similarity coefficient in different populations in Angora goats, F₂， F₃, and H generations were 0.454 0, 0.407 6, 0.611 3 and 0.610 9 respectively, which reached marked difference level between Angora goats with F3 and H generations(P＜0.01). The results of biochemical level and molecular level supported that cross breeding should be applied in F₂ and F₃ generation.