西安荷斯坦奶牛群5个基因座位遗传多态性的PCR-RFLP分析

畜牧兽医学报 ›› 2005, Vol. 36 ›› Issue (6): 545-549.doi:

西安荷斯坦奶牛群5个基因座位遗传多态性的PCR-RFLP分析

张润锋;陈宏;蓝贤勇; 田燚; 潘传英;胡沈荣; 苏利红

1. 西北农林科技大学动物科技学院，陕西省农业分子生物学重点实验室，杨凌 712100；2. 徐州师范大学生物技术研究所，徐州 221116

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2005-06-25 发布日期:2005-06-25

Analysis of Genetic Polymorphisms at Five Loci in Xi’an Population of Chinese Holstein Cattle by PCR-RFLP

ZHANG Run-feng;CHEN Hong;LAN Xian-yong;TIAN Yi;PAN Chuan-ying;HU Shen-rong;SU Li-hong

1.College of Animal Science and Technology, Northwest Science and Technology University of Agriculture and Forestry, Shaanxi Key Laboratory of Agricultural Molecular Biology, Yangling 712100, China; 2.Institute of Biotechnology, Xuzhou Normal University, Xuzhou 221116, China

Received:1900-01-01 Revised:1900-01-01 Online:2005-06-25 Published:2005-06-25

摘要/Abstract

摘要： 应用PCR-RFLP方法对西安荷斯坦牛的κ-cn、β-lg、β-lg 5′侧翼区、CSN1S2、IGFBP-3共5个基因座位进行了多态性分析。结果表明，在西安荷斯坦牛群中，没有发现携带CSN1S2F等位基因的个体，其多态信息含量为0。κ-cn基因座位呈现低度多态（PIC=0.236 6）, β-lg、β-lg 5′侧翼区、IGFBP-3基因座位的多态信息含量分别为0.316 8、0.368 9、0443 9，均呈现中度多态。κ-cn、β-lg、β-lg 5′侧翼区、IGFBP-3、CSN1S2基因座位的杂合度和DNA多态度分别为0.274 2、0.394 7、0.487 9、0.489 1、0和0.025 5、0.011 6、0.033 3、0.011 2、0。而且，在西安荷斯坦牛群中，κ-cn、β-lg、β-lg 5′侧翼区、IGFBP-3共4个基因座位均处于Hardy-Weinberg平衡状态，CSN1S2基因座位处于纯合状态。

Abstract: The genetic polymorphisms of Xi’an population of Chinese Holstein cattle were detected for κ-cn, β-lg, β-lg 5′flanking region, CSN1S2F and IGFBP-3 genes by PCR-RFLP. No carriers of the allele of CSN1S2F were found and its polymorphism information content was 0. κ-cn locus showed low polymorphism (PIC=0.236 6), the three loci of β-lg, β-lg 5′flanking region and IGFBP-3 genes moderate polymorphisms (0.316 8, 0.368 9 and 0.443 9 respectively). The heterozygosity (h) and DNA polymorphisms of the five loci (κ-cn,β-lg,β-lg 5′flanking region, IGFBP-3 and CSN1S2F genes ) were 0.274 2, 0.394 7, 0.487 9, 0.489 1, 0.000 0 and 0.025 5, 0.011 6, 0.033 3, 0.011 2, 0.000 0 respectively. And κ-cn, β-lg, β-lg 5′flanking region and IGFBP-3 loci in the population were at Hardy-Weinberg equilibrium. Only BB genotype was found at CSN1S2F loci.

张润锋;陈宏;蓝贤勇;田燚;潘传英;胡沈荣;苏利红. 西安荷斯坦奶牛群5个基因座位遗传多态性的PCR-RFLP分析[J]. 畜牧兽医学报, 2005, 36(6): 545-549.

ZHANG Run-feng;CHEN Hong;LAN Xian-yong;TIAN Yi;PAN Chuan-ying;HU Shen-rong;SU Li-hong. Analysis of Genetic Polymorphisms at Five Loci in Xi’an Population of Chinese Holstein Cattle by PCR-RFLP[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2005, 36(6): 545-549.

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: https://www.xmsyxb.com/CN/

https://www.xmsyxb.com/CN/Y2005/V36/I6/545