Abstract: In this study, the genetic variation of porcine heart fatty acidbinding protein(HFABP) gene was detected by PCR-RFLP with HinfⅠ and HaeⅢ in 77 pigs of Zhongxu Black Line I. Meanwhile, effects of different PCRRFLP genotypes of H-FABP gene on main growth traits were analyzed in order to investigate whether they have unfavorable effects on growth traits or not during making meat qualities assistant selection by H-FABP gene. The results showed three variant restriction sites of porcine HFABP gene including HinfⅠ-RFLP in 5′-upstream, HaeⅢ-RFLP and HinfⅠ*-RFLP in intron 2, were conformed by PCR-RFLP method. Statistic significant difference(P＜0.05) of product performance (weight of 170 days) was found among animals grouped by different genotype at the HinfⅠ-RFLP site, while groups divided by any other genotypes had no significant differences among all product performance. Conclusion made in this paper will contribute to molecular assistant selection of economic traits including meat quality and production performance in pig breeding.