山羊痘病毒锚蛋白ORF140对NF-κB信号通路的调控作用

doi:10.11843/j.issn.0366-6964.2024.11.034

畜牧兽医学报 ›› 2024, Vol. 55 ›› Issue (11): 5191-5199.doi: 10.11843/j.issn.0366-6964.2024.11.034

山羊痘病毒锚蛋白ORF140对NF-κB信号通路的调控作用

冯秋媛¹^,²(), 余翰维¹^,², 杨云凤¹^,², 张慧², 韩武玮怡², 孙心童², 邵晓龙¹^,², 刘俊林¹, 陈国华², 景志忠²^,*(), 陈轶霞¹^,*()

1. 西北民族大学生命科学与工程学院, 兰州 730030
2. 中国农业科学院兰州兽医研究所/动物疫病防控全国重点实验室/农业部兽医公共卫生重点实验室, 兰州 730046

收稿日期:2024-01-17 出版日期:2024-11-23 发布日期:2024-11-30
通讯作者: 景志忠,陈轶霞 E-mail:fengqiuyuan0808@163.com;zhizhongj@163.com;chenyx69@126.com
作者简介:冯秋媛(1999-), 女, 湖北随州人, 硕士生, 主要从事动物疫病病原分子生物学研究, E-mail: fengqiuyuan0808@163.com
基金资助:
国家自然科学基金项目(32260874)

Regulation of the NF-κB Signaling Pathway by Goat Poxvirus Ankyin Protein ORF140

Qiuyuan FENG¹^,²(), Hanwei YU¹^,², Yunfeng YANG¹^,², Hui ZHANG², Weiyi HANWU², Xintong SUN², Xiaolong SHAO¹^,², Junlin LIU¹, Guohua CHEN², Zhizhong JING²^,*(), Yixia CHEN¹^,*()

1. College of Life Science and Engineering, Northwest Minzu University, Lanzhou 730030, China
2. State Key Laboratory for Animal Disease Control and Prevention, Key Laboratory of Veterinary Public Health of Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China

Received:2024-01-17 Online:2024-11-23 Published:2024-11-30
Contact: Zhizhong JING, Yixia CHEN E-mail:fengqiuyuan0808@163.com;zhizhongj@163.com;chenyx69@126.com

摘要/Abstract

摘要：

NF-κB通路是病毒调控宿主免疫功能的一个重要靶点，在病毒感染致病的过程中发挥重要作用。研究证明，一些痘病毒编码的锚蛋白可通过多种方式调控NF-κB通路，但山羊痘病毒编码的锚蛋白对NF-κB通路有怎样的调控作用尚未有研究报道。为了探究山羊痘病毒编码的锚蛋白ORF140对NF-κB通路的调控作用，作者通过双荧光素酶报告系统检测山羊痘病毒锚蛋白ORF140对NF-κB通路的影响；采用间接免疫荧光检测ORF140在HEK293T细胞中的定位；应用Western blot技术分别检测在NF-κB通路被激活的不同时间点ORF140对NF-κB通路相关因子的影响。结果显示：ORF140能够抑制NF-κB通路；ORF140定位于细胞质中，且在NF-κB通路被激活的情况下也未发生核移位；ORF140能够抑制NF-κB-p65的核移位与p-IκBα的降解。山羊痘病毒锚蛋白ORF140定位在细胞质中，通过抑制NF-κB-p65的核移位与p-IκBα的降解来下调NF-κB通路的活性。

关键词: 山羊痘病毒, 锚蛋白, NF-κB, ORF140

Abstract:

The NF-κB pathway is an important target for regulation of host immune function by viruses and plays an important role in the pathogenesis of viral infections. It has shown that the ankyrin protein encoded by some poxviruses regulates the NF-κB pathway in a variety of ways, but how the ankyrin protein encoded by goat poxvirus (GTPV) regulates the NF-κB pathway remains to be explored. In this paper, we aim to investigate the effects of the ankyrin protein ORF140 encoded by GTPV on the NF-κB pathway. The effects of ankyrin protein ORF140 on the NF-κB pathway were detected using dual luciferase reporter system, its localization in HEK293T cells was observed by indirect immunofluorescence and its effects on expression of the factors associated with the activated NF-κB pathway at different time points by Western blot. Results showed that ORF140 was able to inhibit the NF-κB pathway. It was localized in the cytoplasm and did not undergo nuclear translocation even when the NF-κB pathway was activated. ORF140 was able to inhibit the nuclear translocation of NF-κB-p65 and the degradation of p-IκBα. GTPV ORF140 localizes in the cytoplasm and downregulates NF-κB transcriptional activity by inhibiting the nuclear translocation of NF-κB-p65 and the degradation of p-IκBα.

Key words: goat poxvirus, ankyrin protein, NF-κB, ORF140

中图分类号:

S852.659.1

冯秋媛, 余翰维, 杨云凤, 张慧, 韩武玮怡, 孙心童, 邵晓龙, 刘俊林, 陈国华, 景志忠, 陈轶霞. 山羊痘病毒锚蛋白ORF140对NF-κB信号通路的调控作用[J]. 畜牧兽医学报, 2024, 55(11): 5191-5199.

Qiuyuan FENG, Hanwei YU, Yunfeng YANG, Hui ZHANG, Weiyi HANWU, Xintong SUN, Xiaolong SHAO, Junlin LIU, Guohua CHEN, Zhizhong JING, Yixia CHEN. Regulation of the NF-κB Signaling Pathway by Goat Poxvirus Ankyin Protein ORF140[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(11): 5191-5199.

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山羊痘病毒锚蛋白ORF140对NF-κB信号通路的调控作用

Regulation of the NF-κB Signaling Pathway by Goat Poxvirus Ankyin Protein ORF140

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