畜牧兽医学报 ›› 2022, Vol. 53 ›› Issue (11): 3721-3730.doi: 10.11843/j.issn.0366-6964.2022.11.001
邹惠影, 李俊良, 朱化彬*
收稿日期:
2022-03-17
出版日期:
2022-11-23
发布日期:
2022-11-25
通讯作者:
朱化彬,主要从事家畜胚胎工程与繁殖技术研究,E-mail:zhuhuabin@caas.cn
作者简介:
邹惠影(1987-),女,河北晋州人,博士,主要从事家畜基因编辑与繁殖生物技术研究,E-mail:zouhuiying@caas.cn
基金资助:
ZOU Huiying, LI Junliang, ZHU Huabin*
Received:
2022-03-17
Online:
2022-11-23
Published:
2022-11-25
摘要: 引导编辑系统是基于CRISPR/Cas9系统新开发出的一种基因编辑技术,可以精确实现12种碱基的互换、插入和缺失,并且不需要产生双链断裂和引入外源供体DNA。本文从基因编辑的发展历程、引导编辑系统的组成和特点、引导编辑系统的优化、引导编辑系统在动物和植物研究中的应用、引导编辑系统的设计和脱靶效应等几个方面详细的对引导编辑系统近几年的研究及应用概况进行了综述,为促进科研工作者了解引导编辑系统及进一步推进引导编辑系统在动物和植物科学基础研究和育种方面的应用提供参考。
中图分类号:
邹惠影, 李俊良, 朱化彬. 引导编辑系统的研究与应用进展[J]. 畜牧兽医学报, 2022, 53(11): 3721-3730.
ZOU Huiying, LI Junliang, ZHU Huabin. Progress on Research and Application of Prime Editing System[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(11): 3721-3730.
[1] | CONG L, RAN F A, COX D, et al.Multiplex genome engineering using CRISPR/Cas systems[J].Science, 2013, 339(6121):819-823. |
[2] | JINEK M, CHYLINSKI K, FONFARA I, et al.A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity[J].Science, 2012, 337(6096):816-821. |
[3] | SYMINGTON L S, GAUTIER J.Double-strand break end resection and repair pathway choice[J].Annu Rev Genet, 2011, 45:247-271. |
[4] | DEB S, CHOUDHURY A, KHARBYNGAR B, et al.Applications of CRISPR/Cas9 technology for modification of the plant genome[J].Genetica, 2022, 150(1):1-12. |
[5] | LI G L, LI X Y, ZHUANG S K, et al.Gene editing and its applications in biomedicine[J].Sci China Life Sci, 2022, 65(4):660-700. |
[6] | PERISSE I V, FAN Z Q, SINGINA G N, et al.Improvements in gene editing technology boost its applications in livestock[J].Front Genet, 2021, 11:614688. |
[7] | KOMOR A C, KIM Y B, PACKER M S, et al.Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage[J].Nature, 2016, 533(7603):420-424. |
[8] | GAUDELLI N M, KOMOR A C, REES H A, et al.Programmable base editing of A·T to G·C in genomic DNA without DNA cleavage[J].Nature, 2017, 551(7681):464-471. |
[9] | JIN S, ZONG Y, GAO Q, et al.Cytosine, but not adenine, base editors induce genome-wide off-target mutations in rice[J].Science, 2019, 364(6437):292-295. |
[10] | ZUO E W, SUN Y D, WEI W, et al.Cytosine base editor generates substantial off-target single-nucleotide variants in mouse embryos[J].Science, 2019, 364(6437):289-292. |
[11] | ANZALONE A V, RANDOLPH P B, DAVIS J R, et al.Search-and-replace genome editing without double-strand breaks or donor DNA[J].Nature, 2019, 576(7785):149-157. |
[12] | CHEN P J, HUSSMANN J A, YAN J, et al.Enhanced prime editing systems by manipulating cellular determinants of editing outcomes[J].Cell, 2021, 184(22):5635-5652.e29. |
[13] | DA SILVA J F, OLIVEIRA G P, ARASA-VERGE E A, et al.Prime editing efficiency and fidelity are enhanced in the absence of mismatch repair[J].Nat Commun, 2022, 13(1):760. |
[14] | JIANG T T, ZHANG X O, WENG Z P, et al.Deletion and replacement of long genomic sequences using prime editing[J].Nat Biotechnol, 2022, 40(2):227-234. |
[15] | CHOI J, CHEN W, SUITER C C, et al.Precise genomic deletions using paired prime editing[J].Nat Biotechnol, 2022, 40(2):218-226. |
[16] | ANZALONE A V, GAO X D, PODRACKY C J, et al.Programmable deletion, replacement, integration and inversion of large DNA sequences with twin prime editing[J].Nat Biotechnol, 2022, 40(5):731-740. |
[17] | WANG J L, HE Z, WANG G Q, et al.Efficient targeted insertion of large DNA fragments without DNA donors[J].Nat Methods, 2022, 19(3):331-340. |
[18] | LIU P P, LIANG S Q, ZHENG C W, et al.Improved prime editors enable pathogenic allele correction and cancer modelling in adult mice[J].Nat Commun, 2021, 12(1):2121. |
[19] | XU W, ZHANG C W, YANG Y X, et al.Versatile nucleotides substitution in plant using an improved prime editing system[J].Mol Plant, 2020, 13(5):675-678. |
[20] | XU W, YANG Y X, YANG B Y, et al.A design optimized prime editor with expanded scope and capability in plants[J].Nat Plants, 2022, 8(1):45-52. |
[21] | LU Y M, TIAN Y F, SHEN R D, et al.Precise genome modification in tomato using an improved prime editing system[J].Plant Biotechnol J, 2021, 19(3):415-417. |
[22] | LI X Y, WANG X, SUN W J, et al.Enhancing prime editing efficiency by modified pegRNA with RNA G-quadruplexes[J].J Mol Cell Biol, 2022, 14(4):mjac022. |
[23] | ZHANG G Q, LIU Y, HUANG S S, et al.Enhancement of prime editing via xrRNA motif-joined pegRNA[J].Nat Commun, 2022, 13(1):1856. |
[24] | NELSON J W, RANDOLPH P B, SHEN S P, et al.Engineered pegRNAs improve prime editing efficiency[J].Nat Biotechnol, 2022, 40(3):402-410. |
[25] | LI X S, ZHOU L N, GAO B Q, et al.Highly efficient prime editing by introducing same-sense mutations in pegRNA or stabilizing its structure[J].Nat Commun, 2022, 13(1):1669. |
[26] | LIN Q P, JIN S, ZONG Y, et al.High-efficiency prime editing with optimized, paired pegRNAs in plants[J].Nat Biotechnol, 2021, 39(8):923-927. |
[27] | WOLFF J H, HALDRUP J, THOMSEN E A, et al.piggyPrime:high-efficacy prime editing in human cells using piggyBac-based DNA transposition[J].Front Genome Ed, 2021, 3:786893. |
[28] | EGGENSCHWILER R, GSCHWENDTBERGER T, FELSKI C, et al.A selectable all-in-one CRISPR prime editing piggyBac transposon allows for highly efficient gene editing in human cell lines[J].Sci Rep, 2021, 11(1):22154. |
[29] | WANG Q, LIU J, JANSSEN J M, et al.Broadening the reach and investigating the potential of prime editors through fully viral gene-deleted adenoviral vector delivery[J].Nucleic Acids Res, 2021, 49(20):11986-12001. |
[30] | ADIKUSUMA F, LUSHINGTON C, ARUDKUMAR J, et al.Optimized nickase- and nuclease-based prime editing in human and mouse cells[J].Nucleic Acids Res, 2021, 49(18):10785-10795. |
[31] | LIU B, DONG X L, CHENG H Y, et al.A split prime editor with untethered reverse transcriptase and circular RNA template[J].Nat Biotechnol, 2022, doi:10.1038/s41587-022-01255-9. |
[32] | XU R F, LI J, LIU X S, et al.Development of plant prime-editing systems for precise genome editing[J].Plant Commun, 2020, 1(3):100043. |
[33] | SIMON D A, TÁLAS A, KULCSÁR P I, et al.PEAR, a flexible fluorescent reporter for the identification and enrichment of successfully prime edited cells[J].Elife, 2022, 11:e69504. |
[34] | SCHENE I F, JOORE I P, BAIJENS J H L, et al.Mutation-specific reporter for optimization and enrichment of prime editing[J].Nat Commun, 2022, 13(1):1028. |
[35] | SVRVN D, SCHNEIDER A, MIRCETIC J, et al.Efficient generation and correction of mutations in human iPS cells utilizing mRNAs of CRISPR base editors and prime editors[J].Genes (Basel), 2020, 11(5):511. |
[36] | SCHENE I F, JOORE I P, OKA R, et al.Prime editing for functional repair in patient-derived disease models[J].Nat Commun, 2020, 11(1):5352. |
[37] | GEURTS M H, DE POEL E, PLEGUEZUELOS-MANZANO C, et al.Evaluating CRISPR-based prime editing for cancer modeling and CFTR repair in organoids[J].Life Sci Alliance, 2021, 4(10):e202000940. |
[38] | LIU Y, LI X Y, HE S T, et al.Efficient generation of mouse models with the prime editing system[J].Cell Discov, 2020, 6(1):27. |
[39] | GAO P, LYU Q, GHANAM A R, et al.Prime editing in mice reveals the essentiality of a single base in driving tissue-specific gene expression[J].Genome Biol, 2021, 22(1):83. |
[40] | PETRI K, ZHANG W T, MA J Y et al.CRISPR prime editing with ribonucleoprotein complexes in zebrafish and primary human cells[J].Nat Biotechnol, 2022, 40(2):189-193. |
[41] | BOSCH J A, BIRCHAK G, PERRIMON N.Precise genome engineering in Drosophila using prime editing[J].Proc Natl Acad Sci U S A, 2021, 118(1):e2021996118. |
[42] | LIN Q P, ZONG Y, XUE C X, et al.Prime genome editing in rice and wheat[J].Nat Biotechnol, 2020, 38(5):582-585. |
[43] | TANG X, SRETENOVIC S, REN Q R, et al.Plant prime editors enable precise gene editing in rice cells[J].Mol Plant, 2020, 13(5):667-670. |
[44] | LI H Y, LI J Y, CHEN J L, et al.Precise modifications of both exogenous and endogenous genes in rice by prime editing[J].Mol Plant, 2020, 13(5):671-674. |
[45] | BUTT H, RAO G S, SEDEEK K, et al.Engineering herbicide resistance via prime editing in rice[J].Plant Biotechnol J, 2020, 18(12):2370-2372. |
[46] | HUA K, JIANG Y W, TAO X P, et al.Precision genome engineering in rice using prime editing system[J].Plant Biotechnol J, 2020, 18(11):2167-2169. |
[47] | JIANG Y Y, CHAI Y P, LU M H, et al.Prime editing efficiently generates W542L and S621I double mutations in two ALS genes in maize[J].Genome Biol, 2020, 21(1):257. |
[48] | PERROUD P F, GUYON-DEBAST A, VEILLET F, et al.Prime Editing in the model plant Physcomitrium patens and its potential in the tetraploid potato[J].Plant Sci, 2022, 316:111162. |
[49] | WANG L, KAYA H B, ZHANG N, et al.Spelling changes and fluorescent tagging with prime editing vectors for plants[J].Front Genome Ed, 2021, 3:617553. |
[50] | BHAGWAT A M, GRAUMANN J, WIEGANDT R, et al.multicrispr:gRNA design for prime editing and parallel targeting of thousands of targets[J].Life Sci Alliance, 2020, 3(11):e202000757. |
[51] | HSU J Y, GRVNEWALD J, SZALAY R, et al.PrimeDesign software for rapid and simplified design of prime editing guide RNAs[J].Nat Commun, 2021, 12(1):1034. |
[52] | CHOW R D, CHEN J S, SHEN J, et al.A web tool for the design of prime-editing guide RNAs[J].Nat Biomed Eng, 2021, 5(2):190-194. |
[53] | MORRIS J A, RAHMAN J A, GUO X Y, et al.Automated design of CRISPR prime editors for 56, 000 human pathogenic variants[J].iScience, 2021, 24(11):103380. |
[54] | HWANG G H, JEONG Y K, HABIB O, et al.PE-Designer and PE-Analyzer:web-based design and analysis tools for CRISPR prime editing[J].Nucleic Acids Res, 2021, 49(W1):W499-W504. |
[55] | JIN S, LIN Q P, LUO Y F, et al.Genome-wide specificity of prime editors in plants[J].Nat Biotechnol, 2021, 39(10):1292-1299. |
[56] | KIM D Y, MOON S B, KO J H, et al.Unbiased investigation of specificities of prime editing systems in human cells[J].Nucleic Acids Res, 2020, 48(18):10576-10589. |
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