畜牧兽医学报 ›› 2014, Vol. 45 ›› Issue (12): 1988-1994.doi: 10.11843/j.issn.0366-6964.2014.12.011

• 动物营养 • 上一篇    下一篇

饲粮锌水平对冬毛期水貂血清生化指标和脏器指数的影响

周宁1,2,张海华1,吕智超3,张婷1,黄健1,孙浩然1,尹云厚4,李光玉1*,严昌国2*   

  1. (1.中国农业科学院特产研究所,长春 130112; 2.延边大学农学院,延吉 133002;3.吉林农业大学动物科技学院,长春 130118; 4.贵州民族大学 550025)
  • 收稿日期:2014-03-25 出版日期:2014-12-23 发布日期:2014-12-23
  • 通讯作者: 李光玉,研究员,博士生导师,E-mail:tcslgy@126.com;严昌国,博士生导师,E-mail:ycg@ybu.edu.cn
  • 作者简介:周宁(1984-),男,吉林长春人,博士,主要从事动物遗传育种与繁殖研究,E-mail:14359082@qq.com
  • 基金资助:

    公益性行业(农业)科研专项(200903014);吉林省特种经济动物营养与生理研究创新团队(20121810)

Effects of Diets with Different Zinc Levels on the Selected Serum Biochemical Indexes and Organ Indexes of Minks during the Winter Hair Period

ZHOU Ning1,2,ZHANG Hai-hua1,LYU Zhi-chao3,ZHANG Ting1,HUANG Jian1,SUN Hao-ran1,YIN Yun-hou4,LI Guang-yu1*,YAN Chang-guo2*   

  1. (1.Institute of Special Animal and Plant Sciences,Chinese Academy of Agricultural Sciences,Changchun 130112,China;2.Agriculture College of Yanbian University,Yanji 133002,China;3.College of Animal Science and Technology,Jilin Agricultural University,Changchun 130118,China;4.Guizhou Minzu University,Guiyang 550025,China)
  • Received:2014-03-25 Online:2014-12-23 Published:2014-12-23

摘要:

本试验旨在研究饲粮锌水平对冬毛期水貂血清中生化指标及脏器指数的影响。选取120日龄健康雄性水貂75只,随机分为5组,每组15个重复,每个重复1只水貂,分别饲喂基础饲粮中添加0(Ⅰ组)、50(Ⅱ)、100(Ⅲ组)、300(Ⅳ组)、600 mg•kg-1(Ⅴ组)的试验饲粮。预饲期为7 d,正式期75 d。通过称量体重和内脏重量计算脏器指数。使用生化试剂盒测定血清中蛋白质指标、氮代谢指标、相关锌酶、矿质元素。结果表明,饲粮锌水平对水貂肾脏指数无显著影响(P>0.05);Ⅲ和Ⅳ组心脏指数、肝脏指数和脾脏指数显著高于Ⅰ和Ⅴ组(P<0.05)。Ⅳ组水貂血清中球蛋白(GLOB)和总蛋白(TP)显著高于Ⅰ组(P<0.05),各组之间血清白蛋白(ALB)含量差异不显著(P>0.05);Ⅳ组免疫球蛋白A(IgA)含量显著高于Ⅰ和Ⅱ组(P<0.05),各组之间免疫球蛋白G(IgG)和免疫球蛋白M(IgM)差异不显著(P>0.05);水貂血清尿素氮(UN)各组之间无显著差异(P>0.05);Ⅲ组血清中天门冬氨酸氨基转移酶(AST)显著高于其他各组(P<0.05),Ⅳ组丙氨酸氨基转移酶(ALT)活性显著高于Ⅰ和Ⅱ组(P<0.05);Ⅳ组水貂血清碱性磷酸酶(ALP)活性显著高于Ⅰ、Ⅱ和Ⅲ组(P<0.05),而乳酸脱氢酶(LDH)各组之间差异不显著(P>0.05);水貂血清中锌水平随饲粮锌水平的升高显著升高(P<0.05),血清中钙水平各组之间无显著差异(P>0.05),Ⅴ组血清中磷水平显著低于其他组(P<0.05)。由此可见,饲粮中添加100和300 mg•kg-1锌时脏器指数最佳,能够改善水貂肝和脾功能;饲粮中添加300 mg•kg-1的锌能够提高冬毛期水貂血清中总蛋白和免疫球蛋白含量,以及氮代谢相关酶活性,有利于水貂蛋白质合成及增强机体免疫力。

Abstract:

 This experiment was conducted to study the effects of different dietary zinc level on blood serum biochemical indexes and organ indexes during the winter hair period of mink.75 healthy male minks were randomly divided into 5 groups with 15 replicates per group and 1 mink per replicate.0(Ⅰ),50(Ⅱ),100(Ⅲ),300(Ⅳ),600 mg•kg-1(Ⅴ)of zinc level were added in basical diet.The trial lasted for 75 d with 7 d of pretrial.Organ indexes were calculated by body weight and organ weight.Proteins indices,nitrogen metabolism indices,enzymes,mineral elements of Serum were determined by biochemical kit.The results showed as follows,kidney index were not significantly affected by dietary Zn levels (P>0.05);liver index,spleen index and heart index of group Ⅲ and Ⅳ were significant higher than that of group Ⅰ and Ⅴ (P<0.05).The content of serum total protein and globulin of group Ⅳ were significantly higher than that of group Ⅰ (P<0.05),serum albumin were similar among all groups(P>0.05);The content of IgA in group Ⅳ was significantly higher than that in group Ⅰ and Ⅱ,IgG and IgM were similar among all groups(P>0.05).The content of serum AST in group Ⅲ were significantly higher than that in other groups (P<0.05 ),the content of serum ALT in group Ⅳ were significantly higher than that in group Ⅰ and Ⅱ (P<0.05);Serum UN were similar among all treatments(P>0.05);The contents of Serum ALP in group Ⅳ were significantly higher than those in group Ⅰ,Ⅱ and Ⅲ (P<0.05),and LDH were similar among other groups (P>0.05);Serum Zn increased with dietary zinc level increasing (P<0.05),serum Ca were similar among all groups(P>0.05),serum P in group Ⅴ were significantly higher than in other groups (P<0.05).In conclusion,supplementation of 100 and 300 mg•kg-1 Zn may enhance the organ index of minks .When the dietary Zn level come up to 300 mg•kg-1,the minks in growing period have the relatively higher immunity,protein synthesis rate and bioavailability of Zn.

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