畜牧兽医学报 ›› 2014, Vol. 45 ›› Issue (12): 1917-1923.doi: 10.11843/j.issn.0366-6964.2014.12.002

• 遗传繁育 • 上一篇    下一篇

Kiss-1基因多态性与苏淮山羊产羔数的关联分析

李隐侠1,2,张俊1,2,钱勇1,2,李静心1,2,孟春花1,2,王慧利1,2,钟声1,2,曹少先1,2*   

  1. (1.江苏省农业科学院畜牧研究所,南京 210014;2.江苏省农业科学院动物品种改良和繁育重点实验室,南京 210014)
  • 收稿日期:2014-06-09 出版日期:2014-12-23 发布日期:2014-12-23
  • 通讯作者: 曹少先,研究员,E-mail:caoshaoxian@163.com
  • 作者简介:李隐侠(1979-),女,河南固始人,助理研究员,博士,主要从事动物遗传育种与繁殖方面的研究,E-mail:liyxmh@126.com
  • 基金资助:

    江苏省农业自主创新基金项目[CX(11)1032];公益性行业(农业)科研专项(201303143-06)

Kiss-1 Gene Polymorphisms and Its Association with Litter Sizes in Suhuai Goats

LI Yin-xia1,2,ZHANG Jun1,2,QIAN Yong1,2,LI Jing-xin1,2,MENG Chun-hua1,2,WANG Hui-li1,2,ZHONG Sheng1,2,CAO Shao-xian1,2*   

  1. (1.Institute of Animal Science,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China; 2.Key Laboratory of Animal Breeding and Reproduction,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China)
  • Received:2014-06-09 Online:2014-12-23 Published:2014-12-23

摘要:

为了研究Kiss-1基因多态性与苏淮山羊产羔数之间的关系,本研究采用DNA池测序技术筛选苏淮山羊Kiss-1基因突变位点,采用AS-PCR和PCR-RFLP方法检测130只苏淮山羊Kiss-1基因突变位点的多态性,并分析其与产羔数的关联性。结果表明,在苏淮山羊内含子1中发现2个突变位点g.2270C>T和g.2510A>G,其中g.2510A>G位点有3种基因型AA、AG和GG,基因型频率分别为0.209、0.496和0.295;g.2270C>T位点在苏淮山羊中共检测3种基因型(CC、CT和TT),基因型频率分别为0.143、0.750和0.107。关联分析发现,g.2510A>G位点3种基因型在苏淮山羊各胎产羔数和平均产羔数间差异不显著,g.2270C>T位点中CC型的二胎产羔数显著高于CT和TT型(P<0.05)。单倍型分析发现,g.2510A>G和g.2270C>T位点在苏淮山羊群体中共构建8种单倍型,其中AGCT是主要的单倍型;各单倍型与苏淮山羊产羔数的关联分析发现仅AGCT单倍型的头胎产羔数与AACT单倍型的头胎产羔数间差异显著,其他单倍型在苏淮山羊的各胎产羔数间差异均不显著。综合以上研究表明,Kiss-1基因的g.2270C>T位点可能对苏淮山羊繁殖性状的选育具有一定的指导作用。

Abstract:

To investigate the effects of the polymorphisms of Kiss-1 gene on the reproductive traits of Suhuai goats,the mutations of Kiss-1 gene were screened using DNA pool technology,the polymorphism of Kiss-1 gene in 130 Suhuai goats were detected using AS-PCR and PCR-RFLP methods and its correlation with the litter size of Suhuai goats was analyzed.The results showed that 2 SNPs of g.2270C>T and g.2510A>G were found in Kiss-1 gene intron 1 sequence of Suhuai goats.Three genotypes(AA,AG and GG) were found at g.2510A>G locus in Suhuai goats,and the genotype frequency were 0.209,0.496 and 0.295,respectively;at g.2270C>T locus,3 genotypes(CC,CT and TT) were detected in Suhuai goats and the genotype frequency were 0.143,0.750 and 0.107,respectively.Association analysis showed that there was no significant difference among 3 genotypes (AA,AG and GG) of Suhuai goats in each parity litter size and average litter size at g.2510A>G locus;at g.2270C>T locus,the second parity litter size of CC genotype was significantly higher than those of CT (P<0.05) and CC genotypes(P<0.05).Haplotype analysis found 8 haplotypes and the first parity litter size of AGCT haplotype was significantly higher than that of AACT haplotype,and there existed no significant difference in litter size of Suhuai goats among other haplotypes.Therefore,it can be concluded that g.2270C>T locus of Kiss-1 gene may be a candidate molecular marker for reproductive traits selection in Suhuai goats.

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