鸡体质量性状基因的全基因组关联研究

摘要/Abstract

摘要：

旨在利用全基因组关联分析（GWAS）方法挖掘畜禽各种经济性状相关候选基因及分子标记。本试验利用鸡的60 K SNP芯片，对北京油鸡初生、28、56、80和100日龄体质量进行 GWAS研究。结果表明，共有9个SNPs位点达5%全基因组显著水平，与28或100日龄体质量显著相关，在这些显著位点附近包括LYRM1、LDB2等基因；还有96个SNPs位点达全基因组潜在显著水平，与检测的各个日龄体质量有潜在相关性。这些候选基因和分子标记的揭示为分子标记辅助选择技术的发展积累了素材。

Abstract:

The aim of this study was to use genome-wide association analysis to explore the candidate genes and molecular markers associated with various economic traits. The GWAS of the body weight at d1,d28,d56,d80,d100 of Beijing-You chicken using the Illumina 60K SNP Beadchip was performed. The results showed that there were 9 SNPs reaching 5% Bonferroni genome-wide significance, which were significantly associated with body weight at d28 and d100, including LYRM1, LDB2 and other genes near these SNPs; There were 96 SNPs reaching the significance of “suggestive linkage”, which were potentially associated with each indexes detected. The result revealed that these genes and SNPs laid a foundation for the development of techniques of marker assisted selection.

中图分类号:

S831.2

吴丹，刘冉冉，赵桂苹，郑麦青，张磊，胡耀东，文杰. 鸡体质量性状基因的全基因组关联研究[J]. 畜牧兽医学报, doi: .

WU Dan, LIU Ran-ran, ZHAO Gui-ping, ZHENG Mai-qing, ZHANG Lei, HU Yao-dong, WEN Jie. Genome-wide Association Study of Genes Affecting Body Weight in Chicken[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, doi: .

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: https://www.xmsyxb.com/CN/

https://www.xmsyxb.com/CN/Y2012/V43/I12/1887

参考文献

［1］ZHOU H, DEEBN, CLOVER C M E, et al. Genome-wide linkage analysis to identify chromosomal regions affecting phenotypic traits in the chicken.I.growth and average daily gain1 ［J］.Poult Sci,2006,85(10):1700-1711.

［2］SIWEK M, CORNELISSEN S J B, BUITENHUIS A J, et al. Quantitative trait loci for body weight in layers differ from quantitative trait loci specific for antibody responses to sheep red blood cells ［J］.Poult Sci,2004,83(6):853-859.

［3］TANG S, OU J, SUN D, et al. A novel 62-bp indel mutation in the promoter region of transforming growth factor-beta 2 (TGFB2) gene is associated with body weight in chickens ［J］.Anim Genet,2010,42(1):108-112.

［4］HAN R L, LAN X Y, ZHANG L Z, et al. A novel single-nucleotide polymorphism of the visfatin gene and its associations with performance traits in the chicken ［J］.J Appl Genet, 2010, 51(1):59-65.

［5］张跟喜,丁馥香,是燕萍,等. 肌肉生长抑制素基因(MSTN)外显子1的多态性及其与边鸡生长性状的关联分析［J］.农业生物技术学报,2011,19(1):122-127.

［6］TANG S Q, SUN D X, OU J T, et al. Evaluation of the IGFs (IGF1 and IGF2) genes as candidates for growth, body measurement, carcass, and reproduction traits in Beijing You and Silkie chickens ［J］.Anim Biotechnol, 2010,21:104-113.

［7］FANG M X, NIE Q H, LUO C L, et al. Associations of GHSR gene polymorphisms with chicken growth and carcass traits ［J］.Mol Biol Rep,2010,37:423-428.

［8］侯启瑞,王金玉,王慧华,等.京海黄鸡LYZ基因外显子的遗传多态性及其与体重的关联分析［J］.中国畜牧兽医,2010,37(7):124-127.

［9］卢昕.对猪部分免疫性状的QTL定位及全基因组关联分析［D］.北京:中国农业大学,2006.

［10］HIRSCHHORN J N, DALY M J. Genome-wide association studies for common diseases and complex traits ［J］.Nat Rev,2005,6:95-108.

［11］GU X R, FENG C G, MA L, et al. Genome-wide association study of body weight in chicken F₂ resource population［J］. PLoS One,2011,6(7):e21872.

［12］PURCELL S, NEALE B, TODD-BROWN K, et al. PLINK: a tool set for whole-genome association and population-based linkage analyses ［J］.Am J Hum Genet,2007,81:559-575.

［13］NICODEMUS K K, LIU W, CHASE G A, et al. Comparison of type I error for multiple test corrections in large single-nucleotide polymorphism studies using principal components versus haplotype blocking algorithms ［J］.BMC Genet,2005,6(Suppl. 1):S78.

［14］ZHAO J H. Gap: Genetic analysis package ［J］. J Statistical Software, 2007, 23(8):18.

［15］PRICE A L, ZAITLEN N A, REICH D, et al. New approaches to population stratification in genome-wide association studies ［J］.Nat Rev Genet,2010,11(7):459-463.

［16］WANG Y J, LI J, KWOK A H Y, et al. A novel prolactin-like protein (PRL-L) gene in chickens and zebrafish: Cloning and characterization of its tissue expression ［J］. Gen Comp Endocrinol,2010,166:200-210.

［17］YORK R D, YAO H, DILLON T, et al. Rap1 mediates sustained MAP kinase activation induced by nerve growth factor ［J］.Nature,1998,392:622-626.

［18］LI Y P, HANDBERG K J, JUUL-MADSEN H R, et al. Transcriptional profiles of chicken embryo cell cultures following infection with infectious bursal disease virus ［J］.Arch Virol,2007,152:463-478.

［19］WAHLBERG P, CARLBORG , FOGLIO M, et al. Genetic analysis of an F₂ intercross between two chicken lines divergently selected for body-weight ［J］.BMC Genomics,2009,10:248-260.

［20］ZHU C, LIU Y Q, CHEN F K, et al. LYRM1, a gene that promotes proliferation and inhibits apoptosis during heart development ［J］.Molecules,2010,15:6974-6982.

［21］SAWAI S, KATO K, WAKAMATSU Y, et al. Organization and expression of the chicken N-myc gene ［J］.Mol Cell Biol,1990,10(5):2017.

［22］BERNSTEIN H G, BAUMANN B, DANOS P,et al. Regional and cellular distribution of neural visinin-like protein immunoreactivities (VILIP-1 and VILIP-3) in human brain ［J］.J Neurocytol,1999,28:655-662.

［23］SORANZO N, RIVADENEIRA F, HORSLEY U C, et al. Meta-analysis of genome-wide scans for human adult stature identifies novel loci and associations with measures of skeletal frame size ［J］.PLoS Genet,2009,5(4):e1000445.

［24］SOVIO U, BENNETT A J, MILLWOOD I Y, et al. Genetic determinants of height growth assessed longitudinally from infancy to adulthood in the northern finland birth cohort 1966 ［J］. PLoS Genet,2009,5(3):e1000409.

［25］OSTENDORFF H P, TURSUN B, CORNILS K, et al. Dynamic expression of LIM cofactors in the developing mouse neural tube ［J］. Dev Dynamics, 2006, 235(3):786-791.

［26］JAVERZAT S, FRANCO M, HERBERT J, et al. Correlating global gene regulation to angiogenesis in the developing chick extraembryonic vascular system ［J］.PLoS One,2009,4(11):e7856.

［27］LESTER D H, FARQUHARSON C, PATON I R, et al. A novel integral membrane protein is differentially expressed in the chick growth plate and maps to chromosome 1 ［J］. Anim Genet,1999,30:300-303.

［28］赵秀华,王金玉,张跟喜,等.鸡IGFBP-2基因的多态性及其与生长、繁殖性状的关系［J］.中国兽医学报,2011,38(10):1500-1504.

［29］TIWARI A, WALKER S M K, RAMACHANDRAN R. Cloning and characterization of chicken fat mass and obesity associated (Fto) gene: fasting affects Fto expression ［J］.Elsevier,2011,42(1):1-10.

［30］LI L Q, LEE J Y, GROSS J, et al. A requirement for Lim domain binding protein 1 in erythropoiesis ［J］. J Exp Med, 2010,207(12): 2543-2550.

［31］FORNAGE M, PAPANICOLAOU G, LEWIS C E, et al. Common INSIG2 polymorphisms are associated with age-related changes in body size and high-density lipoprotein cholesterol from young adulthood to middle age ［J］.Metabolism,2010,59(8):1084-1091.

[1]	唐鑫鑫, 郑炬梅, 骆娜, 营凡, 朱丹, 李森, 刘大伟, 安炳星, 文杰, 赵桂苹, 李和刚. 基于全基因组关联分析揭示肉鸡腿病发生的遗传机制[J]. 畜牧兽医学报, 2024, 55(1): 99-109.
[2]	徐小静, 董瑞玲, 魏立民, 赵少猛, 赵桂苹, 张敏红, 冯京海. 文昌鸡母鸡生长模型的研究[J]. 畜牧兽医学报, 2023, 54(12): 4952-4961.
[3]	齐丽娜, 陆雪林, 杨凯旋, 周瑾, 李先玉, 刘珂, 张文刚, 顾欣, 章伟建. 基于SNP芯片分析新浦东鸡的遗传多样性和遗传结构[J]. 畜牧兽医学报, 2023, 54(12): 4962-4971.
[4]	王燕星, 张雨时, 姬海港, 刘阳, 牛玉芳, 韩瑞丽, 刘小军, 田亚东, 康相涛, 李转见. 鸡骨骼肌卫星细胞系的建立及分析[J]. 畜牧兽医学报, 2023, 54(12): 4972-4981.
[5]	范晨宇, 单艳菊, 章明, 姬改革, 巨晓军, 屠云洁, 贺喜, 束婧婷, 刘一帆, 张海涵. 立华麻黄鸡体重和肉品质性状全基因组关联分析[J]. 畜牧兽医学报, 2023, 54(12): 4982-4992.
[6]	陈诚, 乔西波, 孙亿, 康丽, 姜运良. 琅琊鸡FSHR基因-868位点的多态性及对产蛋性能的遗传效应研究[J]. 畜牧兽医学报, 2023, 54(11): 4560-4568.
[7]	金四华, 贾富民, 何培莉, 贾羽晴, 税斐, 刘旭玲, 刘星, 王新, 徐嫚, 耿照玉. IHH过表达对鸡原代软骨细胞增殖和分化的影响[J]. 畜牧兽医学报, 2023, 54(11): 4569-4576.
[8]	陈雪娇, 刘会杰, 臧蕾, 冯静, 鹏达, 张浩. 鸡胚心脏组织转录组数据鉴定雪域白鸡高原低氧适应性关键基因[J]. 畜牧兽医学报, 2023, 54(10): 4154-4163.
[9]	张寅梁, 岳巧娴, 黄晨轩, 陈辉, 王德贺, 周荣艳. 产蛋初期和后期蛋鸡胫骨转录谱的构建及骨代谢相关基因分析[J]. 畜牧兽医学报, 2023, 54(10): 4164-4173.
[10]	郑钢, 连玲. 鸡性别决定及分化关键调控基因DMRT1研究进展[J]. 畜牧兽医学报, 2023, 54(8): 3152-3163.
[11]	白露, 王梦杰, 马小春, 何政肖, 谭晓冬, 刘杰, 赵桂苹, 文杰, 刘冉冉. 一种快速挖掘鸡品种特征性SNP标记集合的方法[J]. 畜牧兽医学报, 2023, 54(8): 3252-3261.
[12]	陈春, 康昭风, 魏岳, 黎观红, 武艳平, 谢金防. Wnt3a基因多态性与崇仁麻鸡皮肤毛囊性状相关性研究[J]. 畜牧兽医学报, 2023, 54(7): 2810-2823.
[13]	刘航, 王欢欢, 葛莹, 张雷, 张伟武, 魏莹晖, 李庆海, 范京辉, 章学东. 基于转录组和蛋白组筛选乌骨鸡肤色性状候选基因[J]. 畜牧兽医学报, 2023, 54(6): 2320-2329.
[14]	王辉, 许梦缘, 刘灵康, 郑喜邦, 李恭贺, 吴文德. PhiC31整合酶电穿孔鸡成纤维细胞诱发位点特异性基因盒交换[J]. 畜牧兽医学报, 2023, 54(6): 2330-2342.
[15]	王政, 郭文婕, 程瑾, 原一桐, 罗榕, 薛毅, 张利环, 朱芷葳, 李慧锋. 营养转运相关基因调控区多态性与黄羽肉鸡饲料转化率关联分析[J]. 畜牧兽医学报, 2023, 54(6): 2343-2352.