大熊猫等8种野生哺乳动物蛔虫的线粒体COXⅠ和COXⅡ基因的亲缘关系分析

畜牧兽医学报

大熊猫等8种野生哺乳动物蛔虫的线粒体COXⅠ和COXⅡ基因的亲缘关系分析

牛李丽¹ ，陈世界²，汪涛³，古小彬³，严玉宝²，余华²，邓家波¹，严慧娟¹，余星明¹，陈维刚¹，王淑贤³，杨光友^3*

（1. 成都动物园，成都 610041； 2. 四川出入境检验检疫局，成都 610041； 3. 四川农业大学动物医学院，雅安 625014）

收稿日期:2012-03-21 出版日期:2012-10-25 发布日期:2012-10-25
通讯作者: 杨光友，教授，博导，主要从事动物寄生虫病学研究，E-mail:guangyou1963@yahoo.com.cn
作者简介:牛李丽（1962-），女，山东淄博人，学士，高级兽医师，从事野生动物疾病临床诊疗与研究工作，E-mail: niulilu621@yahoo.com.cn；陈世界为同等贡献的第一作者
基金资助:
成都大熊猫繁育研究基金项目（CPF-08005）；科技部公益项目（200910188）；国家质检总局项目（2010IK004）

Genetic Relationship of Ascarid Nematodes from Giant Panda and Seven Other Species of Captive Wild Mammals Based on COXⅠ and COXⅡ Genes

NIU Li-li¹, CHEN Shi-jie², WANG Tao³, GU Xiao-bin³, YAN Yu-bao², YU Hua², DENG Jia-bo¹, YAN Hui-juan¹, YU Xing-ming¹, CHEN Wei-gang¹, WANG Shu-xian³, YANG Guang-you^3*

(1. Chengdu Zoological Garden, Chengdu 610041, China; 2. Sichuan EntryExit Inspection
and Quarantine Bureau, Chengdu 610041, China; 3. College of Veterinary Medicine, Sichuan
Agricultural University, Ya’an 625014, China)

Received:2012-03-21 Online:2012-10-25 Published:2012-10-25

摘要/Abstract

摘要： 为了探讨寄生于大熊猫、小熊猫、北极熊、棕熊东北亚种、棕熊西藏亚种、黑熊四川亚种、黑猩猩和白眉长臂猿体内蛔虫的分类地位，采用PCR技术扩增了这些野生动物体内寄生蛔虫的线粒体COXⅠ、COXⅡ基因序列，并与GenBank中注册的同源性序列进行了分析。序列分析结果显示：扩增的8种野生哺乳动物蛔虫的COXⅠ、COXⅡ基因长度均分别为393和582 bp，其中大熊猫与小熊猫及4种熊科动物蛔虫COXⅠ和COXⅡ基因的相似性分别为94.8%~95.0%和94.9%~95.5%；黑猩猩和白眉长臂猿蛔虫的COXⅠ、COXⅡ基因的相似性分别为99.8%和99.5%。分子系统树（NJ/MP/ML）表明，寄生在大熊猫、小熊猫和4种熊科动物体内的蛔虫均为贝蛔属（Baylisascaris）蛔虫；而黑猩猩和白眉长臂猿体内寄生的蛔虫应为蛔属（Ascaris）蛔虫。同时，分析结果也揭示了蛔虫与宿主之间存在协同进化关系。

Abstract: The aim of this study was to investigate the taxonomic relationships of ascarid nematodes from Ailuropoda melanoleuca, Ailurus fulgens, Ursus maritimus, Ursus arctos pruinosus, Ursus thibetanus mupinensis, Ursus arctos lasiotus, Pan troglodytes and Hylobates hoolock. The mitochondrial cytochrome coxidase subunitsⅠ(COXⅠ) and Ⅱ (COXⅡ) were amplified by PCR and sequenced directly. Then, sequence analyses was carried out based on COXⅠ and COXⅡ sequences from ascarid nematodes acquired in our study and 12 homologous sequences from GenBank. The lengths of COXⅠ and COXⅡ in ascarid nematodes from the eight species of captive animals were all 393 bp and 582 bp, respectively. The COXⅠ and COXⅡ of ascarid nematodes from giant panda shared 94.8%-95.0% and 94.9%-95.5% sequence identity with these from A. fulgens, U. maritimus, U. arctos pruinosus, U. thibetanus mupinensis and U. arctos lasiotus, respectively. Additionally, the COXⅠand COXⅡ genes of ascarid nematodes from P. troglodytes shared 99.8% and 99.5% identity with the nematodes from H. hooloc, respectively. Phylogentic analysis showed that ascarid nematodes from A. melanoleuca, A. fulgens and four Ursids species belonged to the genus Baylisascaris, while roundworms from H. hoolock and P. troglodytes clustered under the genus Ascaris. These results showed that a co-evolutionary relationship may link the ascarid nematodes and their corresponding hosts together.

中图分类号:

S852.73

牛李丽,陈世界，汪涛，等. 大熊猫等8种野生哺乳动物蛔虫的线粒体COXⅠ和COXⅡ基因的亲缘关系分析[J]. 畜牧兽医学报, doi: .

NIU Li-li, CHEN Shi-jie, WANG Tao,et al. Genetic Relationship of Ascarid Nematodes from Giant Panda and Seven Other Species of Captive Wild Mammals Based on COXⅠ and COXⅡ Genes[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, doi: .

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