Abstract: The aim of this study was to construct broodiness and laying geese cDNA libraries with SSH, and to identify differentially expressed genes between laying and broody phases. Ovary in broody and laying phases was ascribed to tester and driver for reciprocal SSH procedures. cDNA libraries of specifically or highly expressed genes in broodiness and laying were obtained after twostep hybridization. Positive clones from libraries were randomly selected to validate with PCR. Sequently, 654 positive clones were selected to sequence, qualify, cluster, assemble and compare in GenBank using BLASTn. 166 differentially expressed gene fragments from two libraries showed homology to other known sequences, including 92 functional genes. The expression profiles showed some broodinessspecified genes invovled in apoptosis, cell component, hormonesrelated signal transduction, and some genes involved in metabolism and cell defense in the layingspecified genes library; some ESTs belonged to unknown or hypothetical protein. The result indicate that three genes(prolactin receptor, estrogen receptor α and antimullerian hormone receptor II) in broodinessspecified library are associated to trigger or maintain broodiness in geese based on biological function and signal transduction. The result provide the basis for future revealing the mechanism on broody behavior.