猪H-FABP基因多态性及其与肌内脂肪含量的相关研究

畜牧兽医学报 ›› 2010, Vol. 41 ›› Issue (7): 792-796.doi:

猪H-FABP基因多态性及其与肌内脂肪含量的相关研究

姜延志¹，刘晓研²，李芳琼²，李学伟²*

1. 四川农业大学生命科学与理学院，雅安 625014；2. 四川农业大学动物科技学院，雅安 625014

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-07-20 发布日期:2010-07-20
通讯作者: 李学伟

The Genetic Relationship between Polymorphism of H-FABP Gene andIntramuscular Fat Content in Pig

JIANG Yan-zhi¹, LIU Xiao-yan², LI Fang-qiong², LI Xue-wei²*

1. College of Life Science, Sichuan Agricultural University, Ya′an 625014, China; 2. College of Animal Science and Technology, Sichuan Agricultural University, Ya′an 625014, China

Received:1900-01-01 Revised:1900-01-01 Online:2010-07-20 Published:2010-07-20

摘要/Abstract

摘要： 旨在研究猪H-FABP基因的遗传多态性及其与肌内脂肪含量的遗传效应。利用PCR-RFLP（HinfⅠ、MspⅠ、Hae Ⅲ、Hinf*Ⅰ4种限制性内切酶）分子标记技术检测了中国地方猪种雅南猪、大河猪，培育品种大河乌猪以及杜洛克与长白和约克三元杂交商业群体共148头猪心脏型脂肪酸结合蛋白（H-FABP）基因5′-上游区和第二内含子的遗传变异，并利用固定效应模型分析了H-FABP基因在杜洛克与长白和约克三元杂交商业群体中对肌内脂肪含量的遗传效应。结果表明：（1）在HinfⅠ和MspⅠ位点上，所有4个猪群都存在多态；在Hae Ⅲ位点上，除雅南猪只出现单态外，其余3个猪群都出现多态；在Hinf*Ⅰ位点上，除杜洛克与长白和约克三元杂交商业群体出现多态外，其余3个猪群只表现单态。（2）4个位点对肌内脂肪含量的影响差异显著，各基因型肌内脂肪含量最小二乘均值关系即Hh>HH，bb>Bb>BB，Aa>AA，DD>Dd>dd。结合H-FABP基因的生理功能和已有的研究结果来看，可在特定的群体中将其作为影响猪肌内脂肪含量的候选基因。

Abstract: This experiment was conducted to study the genetic relationship between polymorphism of H-FABP gene and intramuscular fat content in swine. By using 148 pigs from four pig populations including Yanan, Dahe, Dawu and Duroc×(Yorkshire×Landrace), the genetic variations in 5′-upstream region and the second intron in porcine H-FABP gene were detected by PCRRFLP with restriction enzyme HinfⅠ, MspⅠ, Hae Ⅲ, Hinf*Ⅰ. Genetic effect of HFABP gene on intramuscular fat content were analyzed by the fixed model in Duroc×(Yorkshire×Landrace) population. The results showed as follows: (1) 4 pig populations had polymorphism at HinfⅠ and MspⅠ sites; At Hinf*Ⅰ site, Duroc×(Yorkshire×Landrace) pig population had polymorphism, but other pig populations had no polymorphism; At Hae Ⅲ site, 3 pig populations had polymorphism except Yanan pigs. (2) H-FABP gene increased intramuscular in the order of Hh>HH, bb>Bb>BB，Aa>AA，DD>Dd>dd. These results indicated that H-FABP gene could be regarded as candidate gene affecting intramuscular fat content in special pig populations.

姜延志;刘晓研;李芳琼;李学伟. 猪H-FABP基因多态性及其与肌内脂肪含量的相关研究[J]. 畜牧兽医学报, 2010, 41(7): 792-796.

JIANG Yan-zhi;LIU Xiao-yan;LI Fang-qiong;LI Xue-wei. The Genetic Relationship between Polymorphism of H-FABP Gene andIntramuscular Fat Content in Pig[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2010, 41(7): 792-796.

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