畜牧兽医学报 ›› 2010, Vol. 41 ›› Issue (5): 635-638.doi:

• 研究简报 • 上一篇    

不同地区海兰褐蛋鸡中J亚群-禽白血病病毒株gp85基因的分子演化分析

代阳,杨其峰,王波,刘绍琼,王秀臻,柴家前,崔治中,孙淑红*   

  1. 山东农业大学动物医学院,泰安 271018
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-05-25 发布日期:2010-05-25
  • 通讯作者: 孙淑红

Molecular Evolution Analysis for gp85 Gene of Subgroup J-Avian LeukosisViruses Strains from Hyline Egg-type Chickens in Different Areas

DAI Yang, YANG Qi-feng, WANG Bo, LIU Shao-qiong, WANG Xiu-zhen,
CHAI Jia-qian, CUI Zhizhong, SUN Shu-hong*
  

  1. College of Animal Science and College of Veterinary Medicine,Shandong Agricultural University, Taian 271018, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-05-25 Published:2010-05-25

摘要: 本研究通过对不同地区海兰褐蛋鸡群中分离的5株J亚群禽白血病病毒 (ALV-J) 的囊膜糖蛋白基因(gp85)进行同源性分析,阐述了不同海兰褐鸡群中存在的ALV-J的分子演化规律。对2008-2009年分别从北京、陕西、山东泰安、济阳、曲阜等不同地区饲养的海兰褐鸡分离到的5株ALV-J,用PCR方法克隆gp85基因、测序,并与国内外已发表的14株ALV-J gp85基因进行同源性比较。结果表明,5株ALV-J与来自白羽肉鸡的HPRS-103株的同源性最近,平均为96.6% (96.4%~96.8%);与来自国内海兰灰蛋鸡的SD07LK1株的同源性平均仅为89.6%(89.3%~89.9%);而5株ALV-J间的同源性高达98.1%以上(98.1%~100%)。本研究发现,不同地区的海兰褐蛋鸡中广泛存在的ALV-J可能有一个共同的来源,即国外的白羽肉鸡。

Abstract: By the homology analysis for gp85 genes of 5 strains of ALV-J isolated from Hyline laying hens, the molecular evolution of ALV-J was studied in the present study. The 5 strains of ALV-J were obtained from Beijing, Shanxi and Shandong. The gp85 genes were cloned by PCR and the sequence was measured and compared with 14 published reference ALV-J strains. The homology analysis showed that the 5 isolated strains of ALV-J shared 96.6% (96.4%-96.8%) and 89.6% (89.3%-89.9%) homology with the HPRS-103 strain isolated from white meat-type broiler and SD07LK1 strain isolated from egg-type chickens. The homology of the 5 strains was exceeded 98.1% (98.1%-100%). The result indicated that ALV-J strains from Hyline egg-type chickens may have the same origin, the white meat-type broiler chickens imported abroad.