Abstract: To investigate the effect of polysaccharide, flavonoids and saponins of Polysavone on the expression of mouse beta-defensins in RAW264.7 cells, RAW264.7 cells were cultured with Polysavone polysaccharide at 30,60,90,150 μg·mL^-1, Polysavone flavonoids at 10,20,30,50 μg·mL^-1, Polysavone saponins at 10,20,30,50 μg·mL^-1 , respectively. The mRNA expression of mouse beta-defensin (mBD1-3) were detected. The results showed that the mBD2 and mBD3 mRNA expression could be improved by Polysavone polysaccharide, especially the treatment with 30 μg·mL^-1, but there was no effect on the mRNA expression of mBD1. The mBD1 mRNA expression could be improved by Polysavone flavonoids, especially the treatment with 10 μg·mL^-1, the mBD2 and mBD3 mRNA expression could be inhibited. The mBD1 mRNA expression could be improved by Polysavone saponins, the mBD3 mRNA expression could be inhibited, but Polysavone saponins had no effects on the mRNA expression of mBD2. The results showed the different effects of polysaccharide,flavonoids and saponins of Polysavone on the mRNA expression of mBD1,mBD2 and mBD3. The Polysavone polysaccharide may enhance the function of adaptive immunity. The flavonoids and saponins of Polysavone may enhance the function of innate immunity.