Abstract: A subgroup J avian leukosis virus(ALVJ), designated as ZH08, was isolated from broiler breeder flock with a novel hemangioma case in Guangdong Province. The identified results of ELISA test, PCR and immunofluorescence assay (IFA) specific for ALVJ, were all positive. Based on the public fulllength proviral genome sequence of ALVJ prototype strain HPRS103, three pairs of primers were synthesized, and the fulllength proviral genome of ZH08 isolate was sequenced by the method of fragment amplification. The fulllength proviral genome sequence of ZH08 isolate is 7 597 bp, its size has a little difference from that of published fulllength genome sequences, but its structure corresponds with typical retroviral genome structure for completely copy ability, the known oncogenes were not included in its genome. According to the gp85 sequence comparison of ZH08 isolate with those of the other reference strains at home and abroad, the similarity with YZ9901 isolate was the highest(93.7%). Phylogenetic analysis based on gp85 gene showed that ZH08 isolate had the closest linkage to SD07LK1 isolate. This study provide the basis of biological characteristics and pathogenesis research of ZH08 isolate.