基于2DE结合质谱研究围产期奶牛血浆蛋白表达的变化

畜牧兽医学报 ›› 2010, Vol. 41 ›› Issue (2): 188-192.doi:

基于2DE结合质谱研究围产期奶牛血浆蛋白表达的变化

杨永新^1,2，王加启¹*，卜登攀¹，张乐颖¹，李珊珊¹，张春林¹，周凌云¹

1.中国农业科学院北京畜牧兽医研究所动物营养学国家重点实验室，北京 100193；2. 安徽省农业科学院畜牧兽医研究所，合肥 230031

收稿日期:2009-07-31 修回日期:1900-01-01 出版日期:2010-02-24 发布日期:2010-02-24
通讯作者: 王加启

Changes of Plasma Proteins in Transition Dairy Cows Based on 2DE Combination with Mass Spectrometry

YANG Yongxin ^1,2, WANG Jiaqi ¹*, BU Dengpan¹, ZHANG Leying ¹, LI Shanshan¹,ZHANG Chunlin ¹, ZHOU Lingyun ¹

1. State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China; 2. Institute of Animal Science, Anhui Academy of Agricultural Sciences, Hefei 230031, China

Received:2009-07-31 Revised:1900-01-01 Online:2010-02-24 Published:2010-02-24
Contact: WANG Jiaqi

摘要/Abstract

摘要： 本试验旨在揭示奶牛围产期免疫功能下降的机理。采用二维凝胶电泳方法建立了分娩前第21天、分娩后第1和21天血浆蛋白表达图谱，SYPRO ruby染色后，ImageMaster 2D platinum 6.0软件匹配检测差异表达蛋白点，液相色谱串联质谱鉴定。结果表明：与分娩前第21天和分娩后第21天相比，分娩后第1天血浆中转甲状腺素蛋白表达量下调、而结合珠蛋白和α1酸性糖蛋白表达量上调；与分娩前第21天和分娩后第1天相比，分娩后第21天载脂蛋白A1表达量增加，这些表达变化的蛋白涉及急性期应答反应及运输和代谢等生物学功能。结果提示奶牛分娩后急性期蛋白表达量的增加可能与免疫功能下降相关。

Abstract: The mechanism of immune system suppression was investigated in transition dairy cows. Changes of plasma proteins on the 21 d before calving, on the 1 d and 21 d after calving were separated by twodimensional electrophoresis providing a platform for parallel analysis. After visualization proteins with SYPRO ruby, differentially expressed proteins were analyzed by ImageMaster 2D Platinum 6.0 software, and identified by HPLC equipped with ion trap mass spectrometer. The result showed that transthyretin was downregulated on the 1 d after calving, while haptoglobin and alpha1 acid glycoprotein were upregulation, with a much larger and more abrupt variation at parturition, comparative with the 21 d prepartum and postpartum; the expression abundance of apolipoprotein AⅠ increased on the 21 d postpartum compared to prepartum and at parturition. These proteins were involved in acutephase response, transport and metabolism. The findings may provide valuable information for the exploring of the decreased immune function during the transition dairy cows.

杨永新;王加启;卜登攀;张乐颖;李珊珊;张春林;周凌云. 基于2DE结合质谱研究围产期奶牛血浆蛋白表达的变化[J]. 畜牧兽医学报, 2010, 41(2): 188-192.

YANG Yongxin;WANG Jiaqi;BU Dengpan;ZHANG Leying;LI Shanshan;ZHANG Chunlin;ZHOU Lingyun . Changes of Plasma Proteins in Transition Dairy Cows Based on 2DE Combination with Mass Spectrometry[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2010, 41(2): 188-192.

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