蛋白A法压电免疫传感器检测H9亚型禽流感病毒的研究

畜牧兽医学报 ›› 2009, Vol. 40 ›› Issue (8): 1266-1270.doi:

蛋白A法压电免疫传感器检测H9亚型禽流感病毒的研究

詹爱军^1,2，王新卫³，金鑫²，陈书琨²，卢体康²，花群义²，秦智锋²，秦爱建¹*

1.扬州大学兽医学院，扬州 225009；2.深圳出入境检验检疫局动植物检验检疫技术中心，深圳 518010； 3.河南农业大学牧医工程学院，郑州 450002

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-08-24 发布日期:2009-08-24
通讯作者: 秦爱建

Study on SPAPiezoelectric Immunosensor for the Detection of H9 Serotype Avian Influenza Virus

ZHAN Aijun ^1，2, WANG Xinwei ³, JIN Xin ²,CHEN Shukun², LU Tikang ², HUA Qunyi ², QIN Zhifeng ², QIN Aijian ¹*

1. College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China; 2. Technical Center of Animal and Plant Quarantine Inspection,Shenzhen CIQ, Shenzhen 518010,China; 3. College of Animal Husbandry and Veterinary, Henan Agricultural University，Zhengzhou 450002, China

Received:1900-01-01 Revised:1900-01-01 Online:2009-08-24 Published:2009-08-24
Contact: QIN Aijian

摘要/Abstract

摘要：

为建立可以检测H9N2亚型流感病毒的蛋白A法免疫传感器，将石英晶片谐振的高灵敏度与免疫反应的特异性相结合，用蛋白A（SPA）在镀金膜的石英晶体电极偶联抗H9亚型流感病毒的单抗构建传感器探头形成免疫传感器。检测结果表明，该方法具有较好的特异性，不与H5亚型禽流感病毒和NDV反应；检测灵敏度达到20个 EID50；组成的检测器对相应的流感病毒响应良好，该法与鸡胚病毒分离法检出流感病毒的符合率达到91%。本文结果为检测流感病毒免疫传感器的进一步深入研究奠定了基础。

Abstract: By using the high sensitivity of resonance of quartz crystal and specificity of immunoreaction, the gold membrane on quartz crystal electrode was coated with SPA, and the monoclonal antibodies against H9 serotype avian influenza virus(AIV) were coupled with the SPA, then the SPApiezoelectric immunosensor was established and applied for detecting H9 AIV. The detection results showed that the immunosensor displayed better specificity to H9 AIV, and didn’t respond to H5 AIV and NDV. The sensitivity test indicated the detecting limit for the H9 AIV could reach to 20 EID50. The accordance rate between the immunosensor and virus isolation by chicken eggs was 91%. The method provided a foundation for further research on immunosensor for detecting H9 AIV and it could be a new exploration to detect AIV quickly and quantitatively in practical poultry industry.

詹爱军;王新卫;金鑫;陈书琨;卢体康;花群义;秦智锋;秦爱建. 蛋白A法压电免疫传感器检测H9亚型禽流感病毒的研究[J]. 畜牧兽医学报, 2009, 40(8): 1266-1270.

ZHAN Aijun;WANG Xinwei;JIN Xin;CHEN Shukun;LU Tikang;HUA Qunyi;QIN Zhifeng;QIN Aijian . Study on SPAPiezoelectric Immunosensor for the Detection of H9 Serotype Avian Influenza Virus[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2009, 40(8): 1266-1270.

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