Abstract: This experiment was conducted to study permeationenhancing effect of various concentration of Azone and menthol on the in vitro percutaneous penetration of terbinafine(TBF) through canine epidermis. Modified Franz vertical glass diffusion cells were used and excised canine epidermis was chose as transdermal barrier. The in vitro permeation effects of every combination of TBF with various concentration enhancers were evaluated through calculating and comparing the cumulative penetration amount, steadystate flux, permeability coefficient and enhancement ratio. The results revealed that various concentration of enhancers had the permeationenhancing effect, the sequence of effect was 1%AZ**>5%MT**>3% AZ**>3% MT**>1%MT*>5%AZ*. The linear correlation between the effect of combinations of TBF with menthol and the concentration of menthol was positive, while the linear correlation between the effect of combinations of TBF with Azone and the concentration of Azone was negative. Though all combinations of TBF with enhancers had the enhancement effect, the effect of menthol was better than that of Azone. Besides, the menthol is a natural product with low toxicity and side effect, so it is a preferable enhancer for the external preparation.