Abstract:

The aim of the present study was to explore whether the nuclei materials of first polar body (FPB）in porcine oocyte could participate in fertilization and further developed into normal porcine embryo in vitro. The FPBs in MⅡoocytes were obtained by in vitro maturation of the Cumulus oocyte complexes (COCs) from ovary follicles. Live FPB was injected into an enucleated oocyte recipient by micromanipulation after it was isolated from mature oocyte. The oocytes reconstructed by FPB were fertilized by routine in vitro fertilization (IVF) or intracytoplasm sperm injection (ICSI), the recombinant zygotes derived from FPBs were cultured in vitro to observe their ability of further developing in vitro. The results showed that successful rate of recombinant oocytes was as high as about 80%, only about 20% recombined oocytes were degenerated or disaggregated during the process of short culture and fertilization in vitro. Some of recombinant oocytes were successfully developed into twocell, fourcell and eightcell stage embryos, the cleavage rates of twocell stage embryos were 12.9% and 29.8% after routine fertilization and ICSI, respectively. The conclusion was that the nuclei materials of porcine FPB could be able to participate in fertilization and further embryonic development. This might imply the possibility of reproducing normal porcine offspring by using FPBs in future.